BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0099.p (366 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 189 4e-49 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 184 1e-47 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 105 6e-24 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 84 2e-17 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 78 1e-15 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 189 bits (479), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 131/371 (35%), Positives = 194/371 (52%), Gaps = 10/371 (2%) Query: 1 MKIRAAVA-RGDKKIGIESVDLDAPRHTEILVRLVAVGLCHTDVSALEGVLPVP-FPIVL 58 MK RAAVA + K + IE V+L P+ E+LV + A G+CHTD L G P FP +L Sbjct: 1 MKTRAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL 60 Query: 59 GHEGAGVVEAVGESVTGVSAGDHVVVTYD-YCGGCSTCEEHKFANCHNVGPMNFGGSRPD 117 GHEGAGVV VG V + DHV+ Y C C C K C + G PD Sbjct: 61 GHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPD 120 Query: 118 GTSPLSQNGM-MIHGSFFGQSSFASYALCTERNVIKVRKDAPLEMLGPLACGLQTGAGAV 176 TS S +G + H + G S+F++Y + E + K+R DAP + + + CG+ TG GAV Sbjct: 121 ATSRFSLDGKPLFH--YMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAV 178 Query: 177 LNALKVERDSSFAVFGAGAVGLSAVMAAKRAGAKQIIVVDVVESRLTLALELGATNVIDA 236 L KVE ++ VFG G +GL+ + AAK GA +II VD+ +R +A + G T+ I+ Sbjct: 179 LFTAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINP 238 Query: 237 RN-SDALKEILLLSNGGVNFSLDTSGVVQAMEQAVACLAPCGSCGLLAGVDPA-TAIRIY 294 ++ + + I+ +++GG ++S + G M Q++ C ++ GV A I Sbjct: 239 KDVPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTR 298 Query: 295 PFQLLNGRSVRGIIEGETHDPGTFIPAMVDWFMNGEFPFDKLVRF-YSFDEIQSAIADSK 353 PFQL+ GR +G G T +P +VDW+M G+ D L+ + I K Sbjct: 299 PFQLVTGRVWKGSAFGGARGR-TDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMK 357 Query: 354 SGHAIKPILRI 364 SG +I+ ++ Sbjct: 358 SGESIRSVVEF 368 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 184 bits (466), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 123/349 (35%), Positives = 185/349 (53%), Gaps = 15/349 (4%) Query: 1 MKIRAAVARG---------DKKIGIESVDLDAPRHTEILVRLVAVGLCHTDVSALEGVLP 51 MKI+AAV R + + IE +DLD P E+LVR+ A GLCH+D+S + GV P Sbjct: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 Query: 52 VPFPIVLGHEGAGVVEAVGESVTGVSAGDHVVVTY-DYCGGCSTCEEHKFANCHNVGPMN 110 P P+ LGHE + V VG V + AGD VV+ + CG C C E + A C N Sbjct: 61 RPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN 120 Query: 111 FGGSRPDGTSPLSQNGMMIHGSFFGQSSFASYALCTERNVIKVRKDAPLEMLGPLACGLQ 170 G G L +H G S+FA +A+ + R+ +K+ D C + Sbjct: 121 TVGELLSGQRRLRDKTQPVH-HHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVL 179 Query: 171 TGAGAVLNALKVERDSSFAVFGAGAVGLSAVMAAKRAGAKQIIVVDVVESRLTLALELGA 230 TG GA +N +V+ ++ AV G G VGL A++ A +GA+++I VD+ +S+L LA LGA Sbjct: 180 TGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGA 239 Query: 231 TNVIDARNSDALKEILLLSNGGVNFSLDTSGVVQAMEQAVACLAPCGSCGLLAGVD-PAT 289 T ++AR+ DA++++ L+ GGV+++ + +G VQAME A + G + AG+ P Sbjct: 240 TATVNARDPDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYR-MTRRGGMTVTAGLPAPGQ 298 Query: 290 AIRIYPFQLL-NGRSVRGIIEGETHDPGTFIPAMVDWFMNGEFPFDKLV 337 + L+ R+V+G G P IP + F G+ P D+L+ Sbjct: 299 HWALQQVSLVAEERTVKGSYIGSC-VPVRDIPRYIGLFQAGKLPVDRLM 346 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 105 bits (262), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 117/374 (31%), Positives = 163/374 (43%), Gaps = 54/374 (14%) Query: 3 IRAAVARG-DKKIGIESVDLDAPRHTEILVRLVAVGLCHTDVSALEGVLPVP--FPIVLG 59 ++AAV R K + IE V + P +ILV+ A G+CHTD+ A G PV P + G Sbjct: 5 MKAAVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFIPG 64 Query: 60 HEGAGVVEAVGESVTGVSAGDHVVVTYDY--CGGCSTCE---EHKFANCHNVGPMNFGGS 114 HEG G V AVG V V GD V V + + CG CS C E A N G Sbjct: 65 HEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTG------- 117 Query: 115 RPDGTSPLSQNGMMIHGSFFGQSSFASYALCTERNVIKVRKDAPLEMLGPLAC-GLQTGA 173 S NG SFA Y L + V + + P+ C G+ Sbjct: 118 -------YSVNG-----------SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYK 159 Query: 174 GAVLNALKVERDSSFAVFGAGAVGLSAVMAAKRAGAKQIIVVDVVESRLTLALELGATNV 233 G L +V + G G +G AV AK G ++ D+ E +L LA +LGA Sbjct: 160 G--LKETEVRPGEWVVISGIGGLGHMAVQYAKAMGM-HVVAADIHEDKLALAKKLGADLT 216 Query: 234 IDARNSDALKEILLLSNGGVNFSLDTSGVVQAMEQAVACLAPCGSCGLLAGVDPATAIRI 293 +D RN +A+ E+ + GG + +L T+ +AMEQA L G+ L+ G+ P I + Sbjct: 217 VDGRNHNAVAEVQRII-GGAHGALVTAVSPKAMEQAFGFLRARGTMALV-GLPPGD-ISL 273 Query: 294 YPFQLLNGR-SVRGIIEGETHDPGTFIPAMVDWFMNGEFPFDKLVRFYSFDEIQSAIADS 352 F + R +VRG I G D + + + F +DKL D I + A Sbjct: 274 PVFNTVLKRITVRGSIVGTRQDLEESLVFAAEGKVAAHFTWDKL------DNINAIFARM 327 Query: 353 KSGHAIKPILRIDG 366 + G +IDG Sbjct: 328 EEG-------KIDG 334 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 79/269 (29%), Positives = 125/269 (46%), Gaps = 25/269 (9%) Query: 28 EILVRLVAVGLCHTDVSALEGVLPVPFP-IVLGHEGAGVVEAVGESVTGVSAGDHVVVTY 86 ++++++ A +C +D+ G +P +LGHE GVV G ++ + GD VVV + Sbjct: 27 DVILKVTATAICGSDLHLYRGKIPATKAGDILGHEFMGVVVEAGPAIKRLKKGDRVVVPF 86 Query: 87 DY-CGGCSTCEEHKFANCHNVGPMNFGGSRPDGTSPLSQNGMMIHGSFFGQSSF------ 139 CG C C++H +A C + PD + ++ G+ + FG S Sbjct: 87 VIACGQCFFCDQHLYAACET--------TNPDRGALMNAKGIRSGAAMFGYSHLYGGMPG 138 Query: 140 --ASYALCTERNVIKVRKDAPL--EMLGPLACGLQTGAGAVLNALKVERDSSFAVFGAGA 195 A Y + +V +R D+ L E + L+ L TG AV NA V SS A++GAG Sbjct: 139 GQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVKNA-GVREGSSVAIYGAGP 197 Query: 196 VGLSAVMAAKRAGAKQIIVVDVVESRLTLALELGATNVIDARNSDALKEILLLSNG-GVN 254 VG A +A+ GA++I +VD RL A E ++ D + I+ + G GV+ Sbjct: 198 VGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVNFDQVDPAEYIIANTAGRGVD 257 Query: 255 FSLDTSGVV---QAMEQAVACLAPCGSCG 280 +D G +E A+ L GS G Sbjct: 258 GVIDAVGFEAKGSVLETAMTTLKLEGSSG 286 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 73/271 (26%), Positives = 124/271 (45%), Gaps = 36/271 (13%) Query: 16 IESVDLDAPRHTEILVRLVAVGLCHTDVSALE----GVLPVPFPIVLGHEGAGVVEAVGE 71 I+++D A + ++ V++ A G+C +D+ GV+ + P+VLGHE +G + VG Sbjct: 15 IQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEVGT 74 Query: 72 SVTGVSAGDHVVVTYDY-CGGCSTCEEHKFANCHNVGPMNFGGSRPDGTSPLSQNGMMIH 130 +VT ++ G + ++ CG C C+E K +C + M F GS + + Sbjct: 75 AVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLD---MRFYGS--------AMRTPHVQ 123 Query: 131 GSFFGQSSFASYALCTERNVIKVR----KDAPLEMLGPLACGLQT--GAGAVLNALKVER 184 G+F + + ER+ V D M PL+ L AG ++ Sbjct: 124 GAFRQE-------IIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVG------ 170 Query: 185 DSSFAVFGAGAVGLSAVMAAKRAGAKQIIVVDVVESRLTLALELGATNVIDARNSDALKE 244 V G G +G V+AA+RAGA +++V DV L AL++GA + I+ + A + Sbjct: 171 -KRVLVTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALK 229 Query: 245 ILLLSNGGVNFSLDTSGVVQAMEQAVACLAP 275 G + + SG +A+ A+ L P Sbjct: 230 AYAADKGTFDVLFEASGNERALVGALEALRP 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,090,327 Number of extensions: 44150 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 5 Length of query: 366 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 280 Effective length of database: 1,212,660 Effective search space: 339544800 Effective search space used: 339544800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)