BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0098.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 431 e-122 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 256 3e-69 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 251 1e-67 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 227 2e-60 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 224 1e-59 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 431 bits (1109), Expect = e-122, Method: Compositional matrix adjust. Identities = 225/482 (46%), Positives = 316/482 (65%), Gaps = 1/482 (0%) Query: 12 FLEPRAWEGCFYDGKWRPASNRFESCSPSTGKLLAHLGTTSKSELAQAIAVAGKAQLNWA 71 FL+ W+ + G+W A+ P+TG+ L +G S +++ ++ +A AQ W Sbjct: 6 FLDSAQWQQHLFRGQWVAAAQGATVLEPATGRPLTQVGLASPDDVSASVELAIAAQAAWV 65 Query: 72 RQPIAARAEVMRRAAELLALHSEELAGWLVREGGSIRPKAAFEIQSAVDELHHAAGLITH 131 P RA++ RRAA L H +ELA + RE G + K E++ A+ H AAG+ Sbjct: 66 AMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQ 125 Query: 132 SEGQVISSPDPDRLSFSRRLPIGIVGVITPWNVPLLLAMRSVAPALVLGNAVVLKPDPHT 191 ++GQ++ S P RLS +RR P+G++GVI+P+N PL+L +R+VAPAL GNAVV+KPD T Sbjct: 126 AQGQLLPS-TPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRT 184 Query: 192 SVSGGIALARLFEMAGLPEGVFCMTPGGAEVGEALVEAPGVRMITFTGSTHVGRRVGELA 251 VSGG LAR+FE AGLP G+ + PGGAE GEALV APGV MI FTGS VGRR+GELA Sbjct: 185 PVSGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRRIGELA 244 Query: 252 GRNLKRVALELGGNSPMLVLDDCDIKKAVAAGAFGSFHHQGQICMATSRHIVSRAIADQY 311 GR+LK+++LELGG + +++L+D D+ A + A+G++ HQGQICMA +R +V ++ + Sbjct: 245 GRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEGL 304 Query: 312 VELLVQKARSLAVGDPELSDVALGPLINSRQRDRAHDIVTRSIAAGAEIATGGKYNALFY 371 ++ L KAR L VGD VALGP+I+ RQ R H +V S+AAGA++ GG+Y LFY Sbjct: 305 LQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQLFY 364 Query: 372 EPTVLTGVTPGMAAFDDEIFAPVAPIIVAEDDEHALELAAATPYGLAAAIQTGDIGRGLR 431 +PTVL GV PGM F++EIF PVA +I ++DE A+ LA GLAA + + +GR + Sbjct: 365 QPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRAMA 424 Query: 432 LARRLRVGMIHINDQTIVDLPQAPMGGMGQSGNGSRFGSLSSWEDFTEWQWLTASSSPAL 491 +A RLR GM+H+NDQT+ D P GG G +GNG+ G + WE++T+W+W T Sbjct: 425 VAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQQAMP 484 Query: 492 YP 493 YP Sbjct: 485 YP 486 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 256 bits (654), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 166/468 (35%), Positives = 241/468 (51%), Gaps = 9/468 (1%) Query: 22 FYDGKWRPASN--RFESCSPSTGKLLAHLGTTSKSELAQAIAVAGKAQLNWARQPIAARA 79 F +G+W A R +P+ G+LL H+ +E +AI A A W ++ R+ Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 80 EVMRRAAELLALHSEELAGWLVREGGSIRPKAAFEIQSAVDELHHAAGLITHSEGQVISS 139 ++RR EL+ ++++LA + E G +A EI A + A + G I S Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 140 PDPDRLSFSRRLPIGIVGVITPWNVPLLLAMRSVAPALVLGNAVVLKPDPHTSVSGGIAL 199 P P + IG+ ITPWN P + R PAL G +VLKP T S +AL Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSA-LAL 193 Query: 200 ARLFEMAGLPEGVFCMTPGGAE-VGEALVEAPGVRMITFTGSTHVGRRVGELAGRNLKRV 258 A L E AG+P GVF + G + +G + P VR I+FTGST VG+ + + + ++K++ Sbjct: 194 AVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKL 253 Query: 259 ALELGGNSPMLVLDDCDIKKAVAAGAFGSFHHQGQICMATSRHIVSRAIADQYVELLVQK 318 +LELGGN+P +V DD D+ AV + + GQ C+ +R V + D + LV+ Sbjct: 254 SLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEA 313 Query: 319 ARSLAVGDPELSDVALGPLINSRQRDRAHDIVTRSIAAGAEIATGGKYNAL---FYEPTV 375 + VG V GPLIN + + V ++A GA + GGK +AL F+EPTV Sbjct: 314 VKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTV 373 Query: 376 LTGVTPGMAAFDDEIFAPVAPIIVAEDDEHALELAAATPYGLAAAIQTGDIGRGLRLARR 435 L VTP M +E F P+AP+ + DE L LA T +GLA+ + DIGR R+A Sbjct: 374 LADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEG 433 Query: 436 LRVGMIHINDQTIVDLPQAPMGGMGQSGNGSRFGSLSSWEDFTEWQWL 483 L GM+ IN I + AP GG+ QSG G R GS +D+ ++L Sbjct: 434 LESGMVGINTGLISN-EVAPFGGVKQSGLG-REGSHYGIDDYLVVKYL 479 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 251 bits (640), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 159/420 (37%), Positives = 232/420 (55%), Gaps = 6/420 (1%) Query: 78 RAEVMRRAAELLALHSEELAGWLVREGGSIRPKAAFEIQSAVDELHHAAGLITHSEGQVI 137 R ++ AA+ L +++ A + E G+ A F ++ A D L AA + T G+VI Sbjct: 65 RRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAGEVI 124 Query: 138 SSPDPDRLSFSRRLPIGIVGVITPWNVPLLLAMRSVAPALVLGNAVVLKPDPHTSVSGGI 197 S P L+ R P G+V I PWN P++L +R++A L GN VV K + G+ Sbjct: 125 PSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPATHGL 184 Query: 198 ALARLFEMAGLPEGVFCMTPGGAE----VGEALVEAPGVRMITFTGSTHVGRRVGELAGR 253 +A L E AGLP GV E + EA++ P VR + FTGST VGR + R Sbjct: 185 IIAALQE-AGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQCAR 243 Query: 254 NLKRVALELGGNSPMLVLDDCDIKKAVAAGAFGSFHHQGQICMATSRHIVSRAIADQYVE 313 +LK LELGG +P +VL D D+ AV A AFG+F + GQICM+T R IV ++AD++V Sbjct: 244 HLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADEFVR 303 Query: 314 LLVQKARSLAVGDPELSDVALGPLINSRQRDRAHDIVTRSIAAGAEIATGGKYNALFYEP 373 L KA SL +GDP V LG +++ +R + ++ ++A GA + GG+ + Sbjct: 304 RLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTLMPA 363 Query: 374 TVLTGVTPGMAAFDDEIFAPVAPIIVAEDDEHALELAAATPYGLAAAIQTGDIGRGLRLA 433 T+L V+ M + +E F PV PI+ DE A+ A YGL++A+ + D+ R L L+ Sbjct: 364 TLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARALALS 423 Query: 434 RRLRVGMIHINDQTIVDLPQAPMGGMGQSGNGSRFGSLSSWEDFTEWQWLTASSSPALYP 493 RR+ G+ HIN T+ D Q P GG+ SG G FG + +FTE +W+T ++P YP Sbjct: 424 RRIESGICHINGPTVHDEAQMPFGGVKSSGFG-HFGGKAGIAEFTELRWVTVQTTPRHYP 482 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 227 bits (578), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 145/456 (31%), Positives = 239/456 (52%), Gaps = 8/456 (1%) Query: 21 CFYDGKWR-PASNR-FESCSPSTGKLLAHLGTTSKSELAQAIAVAGKAQLNWARQPIAAR 78 F +G+W AS R + +P+T +++ + +++L +A+ A K W R Sbjct: 7 LFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFER 66 Query: 79 AEVMRRAAELLALHSEELAGWLVREGGSIRPKAAFEIQSAVDELHHAAGLITHSEGQVIS 138 +++MRRAA+LL +EE+A + E G +A E A D + A + G+++ Sbjct: 67 SKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVP 126 Query: 139 SPDPDRLSFSRRLPIGIVGVITPWNVPLLLAMRSVAPALVLGNAVVLKPDPHTSVSGGIA 198 + P + P+G V TPWN PL +R ++ AL G ++++K T S Sbjct: 127 ARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPA-E 185 Query: 199 LARLFEMAGLPEGVFCMTPG-GAEVGEALVEAPGVRMITFTGSTHVGRRVGELAGRNLKR 257 L R + AG+P GV + G AE+ E L+ P ++ I+FTGST VG+++ LAG+++KR Sbjct: 186 LIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKR 245 Query: 258 VALELGGNSPMLVLDDCDIKKAVAAGAFGSFHHQGQICMATSRHIVSRAIADQYVELLVQ 317 +ELGG++P +V DD D+ A A F + GQ+C++ +R +V + + + +V+ V Sbjct: 246 ATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVT 305 Query: 318 KARSLAVGDPELSDVALGPLINSRQRDRAHDIVTRSIAAGAEIATGGK---YNALFYEPT 374 A L VG+ + + +GP+ N R+ ++ +++ GA++ TGGK F++PT Sbjct: 306 YAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPT 365 Query: 375 VLTGVTPGMAAFDDEIFAPVAPIIVAEDDEHALELAAATPYGLAAAIQTGDIGRGLRLAR 434 VLT + A ++E F P+A I E + + A PYGLAA LA Sbjct: 366 VLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAA 425 Query: 435 RLRVGMIHINDQTIVDLPQAPMGGMGQSGNGSRFGS 470 R+ GM+ IN + LP+ P GG+ SG GS G+ Sbjct: 426 RVESGMLTINHLGLA-LPELPFGGIKDSGYGSEGGT 460 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 224 bits (572), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 150/424 (35%), Positives = 224/424 (52%), Gaps = 13/424 (3%) Query: 70 WARQPIAARAEVMRRAAELLALHSEELAGWLVREGGS--IRPKAAFEIQSAVDELHHAAG 127 W++ R +++ + A L+ ++ LA R G + P+ ++ +A+ L AAG Sbjct: 60 WSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRM-MDVPNAISHLRAAAG 118 Query: 128 LITHSEGQVISSPD---PDRLSFSRRLPIGIVGVITPWNVPLLLAMRSVAPALVLGNAVV 184 EG+ I + LS++ R PIG+VG I PWN PL++ VA L G VV Sbjct: 119 WANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVV 178 Query: 185 LKPDPHTSVSGGIALARLFEMAGLPEGVFCMTPG-GAEVGEALVEAPGVRMITFTGSTHV 243 +KP T S + LARL + AG P+GV + G G VG AL E P V I+FTGS Sbjct: 179 IKPAEETPQSA-LHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEA 237 Query: 244 GRRVGELAGRNLKRVALELGGNSPMLVLDDCDIKKAVAAGAFGSFHHQGQICMATSRHIV 303 GR + +AG KRV LELGG SP +V DD + A+ G F +QGQ+C A SR +V Sbjct: 238 GRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILV 297 Query: 304 SRAIADQYVELLVQKARSLAVGDPELSDVALGPLINSRQRDRAHDIVTRSIAAGAEIATG 363 R++A ++ + L + A + VGDP V +GP+ Q +R + + + + GA + G Sbjct: 298 QRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAG 357 Query: 364 GKY---NALFYEPTVLTGVTPGMAAFDDEIFAPVAPIIVAEDDEHALELAAATPYGLAAA 420 G F +PT+ GM+ +EIF PV II + +E A+ LA + YGLAA Sbjct: 358 GVSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAAT 417 Query: 421 IQTGDIGRGLRLARRLRVGMIHINDQTIVDLPQAPMGGMGQSGNGSRFGSLSSWEDFTEW 480 + T ++ R R+A ++VG + +N + +D P GG+ SG G R G L+ +TE Sbjct: 418 VWTTNLARAHRVAAGVKVGAVGVNCWSPLD-ANLPWGGIKDSGMG-REGGLAGALAYTEE 475 Query: 481 QWLT 484 + +T Sbjct: 476 KTVT 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,559,720 Number of extensions: 65642 Number of successful extensions: 312 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 248 Number of HSP's successfully gapped: 20 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)