BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0094.p (532 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 200 2e-52 gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 103 3e-23 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 86 7e-18 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 200 bits (509), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 157/519 (30%), Positives = 245/519 (47%), Gaps = 42/519 (8%) Query: 9 VIQVGYGPVSEILAIMLARQGHSVAVFERWTERFPLPRAVCIDHEIYRVLSAIGLRDEL- 67 V+ VG GPV L +L G S + ER + PRAV ID E R + G+ D + Sbjct: 19 VLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGIDDEALRTFQSAGMADAIV 78 Query: 68 PEVSHPAPLYKWFNAEWKELLSIDWAAESISGGPEVHFVHQPTMEAMFDKAVRQYENIDV 127 +V P+ + + A+ + I G + QP EA +R++E++ + Sbjct: 79 RDVIQNVPM-RMYTADRRCFAEIQPTTREF-GWYRRNLFSQPLGEATLRLGLRRFEHVSL 136 Query: 128 NLGSEVMEISQTDEYAEVVVKNVETGATRIVRGRYLIGADGANSKVR-AVIGSQQEDRGF 186 G E++++ Q + + + + E GA +R +Y++G DG S VR ++ E + Sbjct: 137 RSGVELLDLVQDEAGVQASIID-EHGAQATLRAQYVVGCDGGRSTVREKILKLPYEGKTH 195 Query: 187 EADWLVIDLMPNDGVTLDVPAAAQYCNPARPTTIV--PAGVRDGKYYRRWEFMRMPGEAR 244 A W+VI+ DG LD P A +C P RP + P GVR RWEFM PGE Sbjct: 196 PAKWVVIEC---DGDPLDAPYTALHCEPQRPYVCLALPYGVR------RWEFMLFPGEDG 246 Query: 245 EQLESEEMAWKLLEPWVKPDQAV-IVRHKVYTFRSLIAKNWRNKRLLIAGDAAHVMPPFM 303 EQ+ + E +LL V+ A+ I+R +VYT S +A + R+ +AGDAAH+ PP++ Sbjct: 247 EQMLAPEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWI 306 Query: 304 GQGMCAGLRDDWNLAWKLHLVLSGQADEGLLDTYETERRPHASDIVDLSMYLGKVICVPD 363 GQG+ AGLRD +NLAWKL VL G+ LL +Y ER H ++DL+ G V+ + Sbjct: 307 GQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEERHAHVKAMIDLADLFGAVLSQKN 366 Query: 364 PEAAARRDKSFFDKTVTPP----------PPFPSLIAGLLDKDISGAIRPGAGLLGP--- 410 A RDK F P P PS + + +D +R + L+G Sbjct: 367 RLLAWCRDKFFLAIKDIPRVRDYVLQMKFKPMPSFSSQAVMQD---DVRNRSDLVGRMFL 423 Query: 411 HGTVRH-LGVVGRYDDLLGQAFALVVRDAELFSAISKNDLEKLHSLGGVAVHLSKEDD-- 467 V + G V R DDL G F L+ ++ +A ++ + G + + Sbjct: 424 QSLVENGAGEVMRLDDLCGGRFVLLSWRCDVLAAAEPGLRSRMQAFGCDYLQGVRSRSGP 483 Query: 468 ------SVGHAIVDLDGKFSSFMDSQSMKAMLVRPDFYV 500 G I D + K +++ + + +L+RPD ++ Sbjct: 484 RGQVLAGAGVVIQDYENKLNAWFKEKGVDWVLLRPDRFI 522 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 103 bits (257), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 133/548 (24%), Positives = 226/548 (41%), Gaps = 79/548 (14%) Query: 6 IYDVIQVGYGPVSEILAIMLARQGHSVAVFERWTERFPLPRAVCIDHEIYRVLSAIGLRD 65 ++ V+ +G GPV AI LARQG +V + + RA+C + IG Sbjct: 30 VHPVVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTGSRAICFAKRTLDIFDRIGCGQ 89 Query: 66 ELPEVSHPAPLYKWFNAEWKELLSIDWAAESISGGPEVHFVHQPTMEAMFDKAVRQYENI 125 + + K F + + + D ES P + Q +E + +Q+ +I Sbjct: 90 RMVDKGVGWNRGKVFLRD-QAVYEFDLLPESGHARPAFINLQQYYVEGYLLEQAQQWPHI 148 Query: 126 DVNLGSEVMEISQTDEYAEVVVKNVETGATRIVRGRYLIGADGANSKVRAVIGSQQEDRG 185 ++ ++V I+ +++ + V + T ++ RY++ ADG+ S +R ++G + + Sbjct: 149 EIRWKNKVSAIAHHEDHEALTVDTPQGSYT--LKARYVVAADGSRSPLRKMMGLESQGHT 206 Query: 186 FEADWLVIDLMPNDGVTLDVPAAAQ----YCNPARPTTIV-----PAGVRDGKYYRRWEF 236 F +L+ D V +D P A+ + P P V P V + W+ Sbjct: 207 FRDRFLIAD------VKMDAPFPAERWFWFDPPFHPQQSVLLHRQPDDVWRIDFQLGWDA 260 Query: 237 MRMPGEAREQ-------LESEEMAWKLLEPWVKPDQAVIVRHKVYTFRSLIAKNWRNKRL 289 + + E+ L EE+++ L WV VYTF + + +R+ R+ Sbjct: 261 DPVLEKTPERVIPRVRALLGEEVSFSL--EWVS----------VYTFSCVRMERFRHGRV 308 Query: 290 LIAGDAAHVMPPFMGQGMCAGLRDDWNLAWKLHLVLSGQADEGLLDTYETERRPHASDIV 349 L GDAAH + PF +G +G++D NLAWKL VL G+A + LLD+Y +ER +A+D Sbjct: 309 LFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASERE-YAADEN 367 Query: 350 DLSMYLGKVICVP---------DPEAAARRDKSFFDKTVT------------PPPPFPSL 388 L+ P D R+ +F + V P P Sbjct: 368 LLNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQSPLNTPDQ 427 Query: 389 IAGLLDKDISGAIRPGAGLLGPHGTVRHLGVVGRYDDLLGQAFALVVRDA---------E 439 A +G +RPGA + G LG F L++ A + Sbjct: 428 AAAPEAVAFTGPMRPGAPCCD--APLLQAGQPAWLLPQLGDRFVLMIFAAPAQLPAATRQ 485 Query: 440 LFSAISKNDLEKLHSLGGVAVHLSKEDDSV-----GHAI--VDLDGKFSSFMDSQSMKAM 492 +A+++ D +L + A ++ED+ V G AI D+D + D+ Sbjct: 486 ALAALAQPDALRLLFISAQAA--TQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHPGSCY 543 Query: 493 LVRPDFYV 500 L+RPD +V Sbjct: 544 LIRPDQHV 551 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 85.9 bits (211), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 93/369 (25%), Positives = 163/369 (44%), Gaps = 13/369 (3%) Query: 7 YDVIQVGYGPVSEILAIMLARQGHSVAVFERWTERFPLPRAVCIDHEIYRVLSAIGLRDE 66 + V+ +G GP+ A+ A +G V V + RAVC + +G+ Sbjct: 26 HPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIGSRAVCYAKRPLEIWDRLGVAPR 85 Query: 67 LPEVSHPAPLYKWFNAEWKELLSIDWAAESISGGPEVHFVHQPTMEAMFDKAVRQYENID 126 + E L K F E +E+ D E P + + Q +E +A + ID Sbjct: 86 MVEKGVSWKLGKVFFEE-REVYRFDLLPEHAHKMPAMINLQQYYLEQYLVEACQAQPLID 144 Query: 127 VNLGSEVMEISQTDEYAEVVVKNVETGATRIVRGRYLIGADGANSKVRAVIGSQQEDRGF 186 + +++ + Q D +A + V+ + G R+ ++I DGANS R ++G++ + F Sbjct: 145 LRWKHKMISLHQDDTHATLDVETPD-GIFRM-EADWVIACDGANSDTRKMVGAEFSGQFF 202 Query: 187 EADWLVIDLMPNDGVTLDVPAAAQY-CNPA--RPTTIVPAGVRDGKYYRRWEF-MRMPGE 242 + +L+ D++ + D PA + +P R +++ D + R +F + + Sbjct: 203 QDRFLIADVI----MKADFPAERWFWFDPPFHRGQSVLLHKQCDNVW--RIDFQLGWEAD 256 Query: 243 AREQLESEEMAWKLLEPWVKPDQAVIVRHKVYTFRSLIAKNWRNKRLLIAGDAAHVMPPF 302 E+ + E++ ++ K + + VY F R +R+L AGDAAH + PF Sbjct: 257 PEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRYQRVLFAGDAAHQVSPF 316 Query: 303 MGQGMCAGLRDDWNLAWKLHLVLSGQADEGLLDTYETERRPHASDIVDLSMYLGKVICVP 362 +G G++D NL WKL LV+ G+A +D+Y ER A D + S I Sbjct: 317 GARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAADDNLRQSTRSTDFITPK 376 Query: 363 DPEAAARRD 371 + A RD Sbjct: 377 SRASKALRD 385 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,876,763 Number of extensions: 86661 Number of successful extensions: 249 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 243 Number of HSP's successfully gapped: 3 Length of query: 532 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 442 Effective length of database: 1,193,720 Effective search space: 527624240 Effective search space used: 527624240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)