BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0090.p (286 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2... 151 6e-38 gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578... 100 9e-23 gi|300310344|ref|YP_003774436.1| fumarylacetoacetate hydrolase f... 62 7e-11 >gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 151 bits (382), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 93/280 (33%), Positives = 148/280 (52%), Gaps = 15/280 (5%) Query: 1 MKIASYLHQGQASYGVVVDAGVIDASSIAKEIGSSLKDVLANGKLIE---LQRFSEGEKH 57 MK+ Y GQ G++ AG I +++ + +KDV NG +++ L++ + + + Sbjct: 1 MKLLRYGPVGQEKPGILDQAGKI------RDLSAHIKDV--NGAVLDDASLEKIRKLDLN 52 Query: 58 IVPFSEIT--FLPVVSDPGKILCVGINYLSHVKETGREIPKYPMFFTRFADSQIGHEQAM 115 +P E V + GK +C+G+NY H E+ IP P+ F ++ + +G + Sbjct: 53 TLPVVEGNPRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNV 112 Query: 116 IRPFASDKFDFEGELAVVIGKKARHVKAADAVEHVAGYACYNDGSIRDWQ-KHTIQFIPG 174 P S K D+E EL V+IGK ++ DA+ HVAGY ND S R++Q + + G Sbjct: 113 KIPRGSKKTDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKG 172 Query: 175 KNFPKTGGFGPWLVTADEVGDPSQLQLTTRLNGQVVQEAKTDDLIFSIPALIEYCSTFTE 234 K G GPWLVT DEV DP +L + ++G+ Q T +IF++ ++ Y S F Sbjct: 173 KGCDTFGPIGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMS 232 Query: 235 LNPGDVIITGTTGGVGAFREP-PLWMKEGDVVEVEISRIG 273 L PGDVI TGT GVG +P ++++ G + + I +G Sbjct: 233 LQPGDVISTGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLG 272 >gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578 [Herbaspirillum seropedicae SmR1] Length = 227 Score = 100 bits (250), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 63/182 (34%), Positives = 94/182 (51%), Gaps = 6/182 (3%) Query: 75 KILCVGINYLSHVKETGREIPKYP-MFFTRFADSQIGHEQAMIRPFASDKFDFEGELAVV 133 +I CVG NY +H +E G + + P FF + AD+ + + + P A+ + E EL V Sbjct: 25 RIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVVPTDSVVPYPVATSDYHHEIELVVA 84 Query: 134 IGKKARHVKAADAVEHVAGYACYNDGSIRDWQKHTIQ----FIPGKNFPKTGGFGPWLVT 189 + K R++ A+E V GYA D + RD Q + + K F ++ P +V Sbjct: 85 LAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAKKLGRPWDMAKGFDQSAPCAP-IVP 143 Query: 190 ADEVGDPSQLQLTTRLNGQVVQEAKTDDLIFSIPALIEYCSTFTELNPGDVIITGTTGGV 249 + G P Q + ++NG+V Q+ DLI+S+ I Y S EL PGD+I TGT GV Sbjct: 144 VAQAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETISYLSGLVELFPGDLIFTGTPEGV 203 Query: 250 GA 251 GA Sbjct: 204 GA 205 >gi|300310344|ref|YP_003774436.1| fumarylacetoacetate hydrolase family protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 61.6 bits (148), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 51/165 (30%), Positives = 77/165 (46%), Gaps = 17/165 (10%) Query: 94 IPKY---PMFFTRFADSQIGHEQAMIRPFASDKFDFEGELAVVIGKKARHVKAADAVEHV 150 IP + P+ + +D +G Q + P D DFEGE V++G V+A+ A++ V Sbjct: 104 IPHFDTVPVMYQGASDDFLGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQASQALQAV 163 Query: 151 AGYACYNDGSIRDWQKHTIQ----FIPGKNFPKTGGFGPWLVTADEVGDPS------QLQ 200 ND S+R H ++ F+ K P T F P VT DE+G P+ Q++ Sbjct: 164 RLLVQINDWSLRALGPHEMKTGFGFLQAK--PST-AFAPMAVTPDELG-PAWRDGRVQMR 219 Query: 201 LTTRLNGQVVQEAKTDDLIFSIPALIEYCSTFTELNPGDVIITGT 245 L + NGQ ++ FS LI + + L G VI +GT Sbjct: 220 LQVQWNGQPFGHPHGGEMNFSFGELIAHAARSRRLTAGTVIGSGT 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 883,575 Number of extensions: 35580 Number of successful extensions: 82 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 79 Number of HSP's successfully gapped: 3 Length of query: 286 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 202 Effective length of database: 1,222,130 Effective search space: 246870260 Effective search space used: 246870260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)