BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0068.p (469 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 700 0.0 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 159 4e-40 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 131 1e-31 gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 120 2e-28 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 109 5e-25 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 700 bits (1807), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/451 (78%), Positives = 398/451 (88%), Gaps = 5/451 (1%) Query: 13 MSYQARQRWFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT 72 M + + WFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT Sbjct: 3 MKVRTQSGWFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT 62 Query: 73 RAALTHSAPEMRRELLFDLASNEGIRIYPLEKTDKITPPDDSPVLPELEFYVKQAMGDDT 132 RAALTHSAPEMRRELLFDLASNEGIRIYPLEK+D+I PP+DSP++PELE+YV+Q++ ++T Sbjct: 63 RAALTHSAPEMRRELLFDLASNEGIRIYPLEKSDRIVPPEDSPIVPELEYYVRQSLDENT 122 Query: 133 KFASGVNDVDGFWISFKIDEDEYWLMLDRGRLDRTSSLQWLGWGSIALVLSLIGAVFIST 192 FAS VNDV+GFWISF ID+D+YWLMLDRGRLDRTSSLQWLGWGSIAL LSLIGAVFIST Sbjct: 123 LFASSVNDVEGFWISFNIDDDKYWLMLDRGRLDRTSSLQWLGWGSIALFLSLIGAVFIST 182 Query: 193 LINQPLARLTAAARAIAKGKQPDPLPESGPTEIREANLSFNQMVADLDRIESDRAVILAG 252 LINQPLARLTAAARAIAKG+QP+PLPESGPTEI EAN SFNQMVADL+RIESDRA+ILAG Sbjct: 183 LINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAG 242 Query: 253 ISHDLRTPLARMQLEVEMAKLSDEARDGMHSDLAQMDAIIGQFLDYAKPFDSSSFTPVDL 312 ISHDLRTPLARMQLEVEMA LSDEARDGMHSDLAQMD+IIGQFLDYAKPFD+SS +DL Sbjct: 243 ISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLDYAKPFDTSSLETIDL 302 Query: 313 SGLLEDAANNSKRLNDVMVSTAISPDIEVMGNTVELERIISNLIENARRYGKTAGTDVAN 372 + +L+D A N+ RL+D+ V T I+PD +V GN EL R+ SNL+ENARRYGKT GTD A Sbjct: 303 ADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTELARVFSNLVENARRYGKTEGTDCAE 362 Query: 373 IDILCKIEDRHVKVEISDHGPGAPDSELEHLLRPFTRLDTARGQANGSGLGLAIVNRIVI 432 IDI +IE V VE++DHGPG P +E E LLRPFTRLDTARGQANGSGLGLAIVNRIV+ Sbjct: 363 IDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPFTRLDTARGQANGSGLGLAIVNRIVL 422 Query: 433 RHNGKLALRNRDG--GGLVVQMMFPTVPIRG 461 +HNGKL L+ + GL+VQ+ T+P++G Sbjct: 423 KHNGKLLLKGHEETRSGLLVQI---TLPLQG 450 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 159 bits (402), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 145/470 (30%), Positives = 216/470 (45%), Gaps = 52/470 (11%) Query: 22 FSNGLFWRTFFLLTFLITASMVA--WVA--SFRMVERGPRAEQLAAQIVSVVTITRAALT 77 F + R FFLL I ++ A W+A R R R + LA ++ +V Sbjct: 4 FLGSIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAV----- 58 Query: 78 HSAPEMRRELLFDLASNEGIRIYPLEKTDKITPPD--DSPVLPELEFYVKQAMGDDTKFA 135 + P +R ++ ASN G +E + PD SP L ++ +G+D K A Sbjct: 59 DNIPPAQRPIVLQAASNFGFEARMVEDMNDAIGPDASPSPSTSPLTEMLQARLGEDRKIA 118 Query: 136 SG---------VNDV-----DGFWISFKIDEDEYWLMLD----------RGRLDRTSSLQ 171 +D D + + D+ + L R R Sbjct: 119 VQRESECPPLRTDDARAPRRDTCRVIYISLHDQALMRLRLHQPGEPPALRARAGPGGPAS 178 Query: 172 WLGWGSIALVLSLIG--AVFISTLINQPLARLTAAARAIAKGKQPDPLPESGPTEIREAN 229 G I L L LI A ++ + +P+ +L AA + PL E+GPTEIR+A Sbjct: 179 LFGLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAA 238 Query: 230 LSFNQMVADLDRIESDRAVILAGISHDLRTPLARMQLEVEMAKLSDEA-RDGMHSDLAQM 288 +FN M A + R R +LA I+HDL+TPL RM+L +E K+SD + + DL M Sbjct: 239 HAFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLE--KVSDPGLQQKLIDDLGVM 296 Query: 289 DAIIGQFLDYAKPFDSS-SFTPVDLSGLLED-AANNSKRLNDVMVSTAISPDIEVMGNTV 346 ++ + LD A+ DSS +D+ LL+ A+ + DV + S VM Sbjct: 297 HGMVREGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQG--STRAFVMAQPG 354 Query: 347 ELERIISNLIENARRYGKTAGTDVANIDILCKIEDRHVKVEISDHGPGAPDSELEHLLRP 406 L R ++NLI+NA +YGK+A +A +E + + + I D GPG P+SELE + P Sbjct: 355 ALRRCLTNLIDNACKYGKSARVSIA-------LEQQKIAIRIRDQGPGIPESELEAVFEP 407 Query: 407 FTRLDTARGQ-ANGSGLGLAIVNRIVIRHNGKLALRNRDGGGLVVQMMFP 455 F RL+ +R + G+GLGL I I H+G+L LRN GGL V + P Sbjct: 408 FYRLEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELP 457 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 131 bits (329), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 91/268 (33%), Positives = 136/268 (50%), Gaps = 18/268 (6%) Query: 193 LINQPLARLTAAARAIAKGKQPDPLPESGPTEIREANLSFNQMVADLDRIESDRAVILAG 252 L +PL + AA + + PL GP E+ A +FN M + ++R LA Sbjct: 224 LAMKPLQVMAEAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQRRIIDNIAERTRFLAA 283 Query: 253 ISHDLRTPLARMQLEVEMAKLSDE-ARDGMHSDLAQMDAIIGQFLDYAKPFDSSSFTP-V 310 +SHDLR+P+ RM+L EM L DE ++ DL +MDA+I L+ + D + Sbjct: 284 VSHDLRSPITRMKLRTEM--LPDERGKEKFRKDLDEMDALISATLNVVQDIDVQERPQLI 341 Query: 311 DLSGLLEDAANNSKRL-NDVMVSTAISPDIEVMGNTVELERIISNLIENARRYGKTAGTD 369 DL LLE + + +V +S +P + G L R + NL+ENA RYG+ A Sbjct: 342 DLDSLLESLRTDQAEVGGEVTLSGHAAP---LQGYPRSLRRCLQNLVENAVRYGQRARVQ 398 Query: 370 VA-NIDILCKIEDRHVKVEISDHGPGAPDSELEHLLRPFTRLDTARGQANGS-GLGLAIV 427 + + D +C + + D GPG P++ LE + PF RL+ +R G GLGL+I Sbjct: 399 LQDDADQVC--------IRVDDDGPGIPEALLERVQEPFYRLERSRNAGTGGFGLGLSIA 450 Query: 428 NRIVIRHNGKLALRNRDGGGLVVQMMFP 455 +V H G + L NR GGGL V+++ P Sbjct: 451 ATVVQAHRGVMKLVNRPGGGLRVEILIP 478 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 84/251 (33%), Positives = 123/251 (49%), Gaps = 10/251 (3%) Query: 208 IAKGKQPDPLPESGPTEIREANLSFNQMVADLDRIESDRAVILAGISHDLRTPLARMQLE 267 KG+ LPE+G E + +F+ M + R DR + ISHDLRTP+ R++L Sbjct: 233 FGKGETVPSLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLR 292 Query: 268 VEMAKLSDEARDGMHSDLAQMDAIIGQFLDYAKPFD-SSSFTPVDLSGLLEDAANNSKRL 326 E+ D R H DL ++D ++ L K D + T V L L+ +++ Sbjct: 293 TELMD-DDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLA 351 Query: 327 NDVMVSTAISPD-IEVMGNTVELERIISNLIENARRYGKTAGTDVANIDILCKIEDRHVK 385 A +P I V+ + L+R I NL +NA YG+ +I E V+ Sbjct: 352 GH---EIAFTPSGINVIAKPLALKRAIGNLFDNALYYGQKVEI---SIRPSPGAEGDLVE 405 Query: 386 VEISDHGPGAPDSELEHLLRPFTRLDTARGQ-ANGSGLGLAIVNRIVIRHNGKLALRNRD 444 +EI DHGPG P+ + L +P+ RL+ R Q + G GLGL I IV H G+L LRN Sbjct: 406 IEIRDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHP 465 Query: 445 GGGLVVQMMFP 455 GGL+ ++ P Sbjct: 466 EGGLMATIILP 476 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 109 bits (272), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 87/292 (29%), Positives = 146/292 (50%), Gaps = 18/292 (6%) Query: 178 IALVLSLIGAVFISTLINQPLARLTAAARAIAKGKQPDPLPESGPTEIREANLSFNQMVA 237 +A++L+ V IS ++ +PL R+ A +A G + E+ FNQ+ Sbjct: 180 VAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDEVGMLAQDFNQLAM 239 Query: 238 DLDRIESDRAVILAGISHDLRTPLARM--QLE-VEMAKLSDEARD--GMHSDLAQMDAII 292 L+R E R +A +SH+LRTPLA + QLE +E L +A+ + S++ ++ ++ Sbjct: 240 TLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSEVEHLNKLV 299 Query: 293 GQFLDYAKPFDSSSFT----PVDLSGLLED-AANNSKRLND--VMVSTAISPDIEVMGNT 345 G D + D+ + T +DL+ LL D AA S R + + +S + + V G+ Sbjct: 300 GDLYDLSLA-DAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMPVHGDA 358 Query: 346 VELERIISNLIENARRYGKTAGTDVANIDILCKIEDRHVKVEISDHGPGAPDSELEHLLR 405 L++++SNL EN+ RY G + + ++ +H + D PG P + L Sbjct: 359 ARLQQLLSNLFENSLRYTDAGG----RLQVHAHLQQQHWHLVFDDSEPGVPPEVMARLFE 414 Query: 406 PFTRLDTARGQAN-GSGLGLAIVNRIVIRHNGKLALRNRDGGGLVVQMMFPT 456 F R++ +R +A+ G+GLGLAI RIV H G + GGL V ++ P Sbjct: 415 RFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPA 466 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,456,343 Number of extensions: 60040 Number of successful extensions: 324 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 33 Length of query: 469 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 380 Effective length of database: 1,198,455 Effective search space: 455412900 Effective search space used: 455412900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)