BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0001.p (370 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane ... 185 5e-48 gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 87 2e-18 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 73 4e-14 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 66 5e-12 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 59 7e-10 >gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 376 Score = 185 bits (470), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 134/363 (36%), Positives = 195/363 (53%), Gaps = 11/363 (3%) Query: 2 RQAIALCLAVSALAACSKKVEKVEEIRPVRAMVIAA---GNADVVAEFPGEVRARVESRL 58 R+ L V LAACSK + R V +V+ A G A + P EV++R + + Sbjct: 15 RRVYPLLALVITLAACSKPAPVADAPREV--VVLQAQQRGQAGTL-HLPAEVQSRYVTSM 71 Query: 59 GFRVGGKIVARKVDVGSIVRRGQVLMQLDPQDLQLAQRQADAAMKSALSNRDLASAELKR 118 FR+ G++ R+V +G +VR+GQVL +LDP D A A + SA + D A +L+R Sbjct: 72 SFRIAGQLAERRVHLGDVVRKGQVLARLDPADANQNASAAQAELASARQHLDAAEKQLQR 131 Query: 119 YQELRDKNFVSQATLDSKQTAYQSALSGYDQAVASLRNQSNQTGYAVLTADVDGVVTAVD 178 + + +S L+ Q A+ +ALS + A A NQ Y L A DGV++A Sbjct: 132 DVQQAREALISPQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDYTTLVAQHDGVISAEQ 191 Query: 179 AEAGQVVAAGTPVVSVAQAGDKDVVIGIPEDKVDVLRRSADVRVRLWANPKETIVGKIRE 238 A G V+AAG PV S+A +G DVV + + +V L+ A V L A P +T GK+RE Sbjct: 192 ANTGDVLAAGQPVYSLAWSGQVDVVTEVADRQVATLQPGAAAVVTLAALPGKTFTGKVRE 251 Query: 239 VSPIADAATRTYTFKIALPDSAKDVRLGMTAYVAF--AGKTSGEMIRLPLTALYPVDGKT 296 VSP ADA +RTY K+ L VRLGMT VA A ++ ++ LP TAL+ + Sbjct: 252 VSPAADAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASSAAPVLLLPATALFHQGDRP 311 Query: 297 SVWLV-ENGAVKVVPVQLGGQSGNDVLISAGLTAGQTVVTAGVNLLKPGQKVKILGAEPL 355 ++W+V E G +++ PV + + +S G+TA VV GV+ L G+KVK + PL Sbjct: 312 ALWVVGEQGQLELRPVTVAAYGERSISVSQGVTASDKVVVQGVHTLTAGEKVKPVA--PL 369 Query: 356 AAD 358 A+ Sbjct: 370 HAE 372 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 75/311 (24%), Positives = 148/311 (47%), Gaps = 2/311 (0%) Query: 46 FPGEVRARVESRLGFRVGGKIVARKVDVGSIVRRGQVLMQLDPQDLQLAQRQADAAMKSA 105 PG ++ V+S + R GG + D+G+ V++G++L +LD ++ QA AA + A Sbjct: 86 LPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQALAAREQA 145 Query: 106 LSNRDLASAELKRYQELRDKNFVSQATLDSKQTAYQSALSGYDQAVASLRNQSNQTGYAV 165 S+ +LA + + R++ LR K+ VSQ LD +++A+ A + A A++ G+ Sbjct: 146 ASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASANLAAADANVGRLRQVEGFKR 205 Query: 166 LTADVDGVVTAVDAEAGQVV--AAGTPVVSVAQAGDKDVVIGIPEDKVDVLRRSADVRVR 223 + A G++T + + G ++ AG + ++Q V + +P+ ++++ V V Sbjct: 206 IVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQSYANLVKPGQQVTVT 265 Query: 224 LWANPKETIVGKIREVSPIADAATRTYTFKIALPDSAKDVRLGMTAYVAFAGKTSGEMIR 283 GK+ + DAATR+ ++ L ++ + G VA K SG ++ Sbjct: 266 QSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQVALPIKPSGALLV 325 Query: 284 LPLTALYPVDGKTSVWLVENGAVKVVPVQLGGQSGNDVLISAGLTAGQTVVTAGVNLLKP 343 T ++ DG + + V++ ++LG G V + GL + ++ + L Sbjct: 326 AADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGLQGDEHLILNPSDALAE 385 Query: 344 GQKVKILGAEP 354 G +V+ +P Sbjct: 386 GDQVEPTLEQP 396 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 73.2 bits (178), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 87/366 (23%), Positives = 151/366 (41%), Gaps = 21/366 (5%) Query: 12 SALAACSKKVEKVEEIRP---VRAMVIAAGNADVVAEFPGEVRARVESRLGFRVGGKIVA 68 +AL AC +K P V + IA + E PG V A +++ RV G + Sbjct: 18 AALVACGQKPAAQGGPMPPSEVSVVTIAPQRVAITNELPGRVEAFRVAQVRARVAGIVQK 77 Query: 69 RKVDVGSIVRRGQVLMQLDPQDLQLAQRQADAAMKSALSNRDLASAELKRYQELRDKNFV 128 R GS V+ GQVL ++DP + + A A + A +N + +RY+ L + N V Sbjct: 78 RVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAV 137 Query: 129 SQATLDSKQTAYQSALSGYDQAVASLRNQSNQTGYAVLTADVDGVVTAVDAEAGQVVAAG 188 S+ D+ A + A + A+++ S GYA +T+ + G + G +V G Sbjct: 138 SKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQG 197 Query: 189 TPV-VSVAQAGDKDVVIGIPEDKVDVLR---------------RSADVRVRLWANPKETI 232 +++ Q D V + + + D+L+ A V + L + Sbjct: 198 DATQLALVQQLD-PVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKASVTLELENGQAYPL 256 Query: 233 VGKIREVSPIADAATRTYTFKIALPDSAKDVRLGMTAYVAFAGKTSGEMIRLPLTALYPV 292 GK+ D T + + + P+ + + G + + +P A+ Sbjct: 257 PGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRD 316 Query: 293 DGKTSVWLVE-NGAVKVVPVQLGGQSGNDVLISAGLTAGQTVVTAGVNLLKPGQKVKILG 351 G ++V +V V+ PV+ GG GN+ + GL G V+ G+ ++PG VK + Sbjct: 317 AGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATVKPVQ 376 Query: 352 AEPLAA 357 P A Sbjct: 377 WNPAGA 382 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 65.9 bits (159), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 78/304 (25%), Positives = 142/304 (46%), Gaps = 23/304 (7%) Query: 61 RVGGKIVARKVDVGSIVRRGQVLMQLDPQDLQLAQRQADAAMKSALSNRDLASA--ELKR 118 RV G+++ G +V+ G +L ++DP+ LQ A QA+ + A LASA +LKR Sbjct: 65 RVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQL--ARDRALLASARLDLKR 122 Query: 119 YQELRDKNFVSQATLDSKQT---AYQSALSGYDQAVASLRNQSNQTGYAVLTADVDGVVT 175 YQ L ++ ++ +D++ Y+ + + VAS R Q + +TA + G + Sbjct: 123 YQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASAR---LQLSFTRVTAPISGRLG 179 Query: 176 AVDAEAGQVVAAG--TPVVSVAQAGDKDVVIGIPEDKV----DVLRRSADVRVRLWANPK 229 A+ G V +V + Q + IPED + L+ + + W + Sbjct: 180 LRQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPAQAWDREQ 239 Query: 230 ETIV--GKIREVSPIADAATRTYTFKIALPDSAKDVRLGMTAYVAFAGKTSGEM--IRLP 285 + + G + + + DA T T K P++ D L + +V + + E +P Sbjct: 240 KNKLADGVLLTIDNVVDATTGTVKLKAQFPNT--DYALFPSQFVNIRLQLNTEQGATVIP 297 Query: 286 LTALYPVDGKTSVWLV-ENGAVKVVPVQLGGQSGNDVLISAGLTAGQTVVTAGVNLLKPG 344 A+ V++V ++ +V V PV+ G + +I+ G++AG+TVV G++ L+ G Sbjct: 298 TAAIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRLREG 357 Query: 345 QKVK 348 KV+ Sbjct: 358 AKVE 361 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 58.9 bits (141), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 76/288 (26%), Positives = 126/288 (43%), Gaps = 26/288 (9%) Query: 2 RQAIALCLAVSALAACSKKVEKVEEIR--PVRAMVIAAGNADVVAEFPGEVRARVESRLG 59 R A++L L V+ LAACS+ ++ + V +V+ A + E PG A + + + Sbjct: 20 RLALSLIL-VAVLAACSRSGDQPKPTAQAEVGYVVVKAQRQALTTELPGRTTAYLAADIR 78 Query: 60 FRVGGKIVARKVDVGSIVRRGQVLMQLDPQDLQLAQRQADAAMKSALSNRDLASAELK-- 117 +VGG I R GS V+ GQ L Q+DP Q A A A++ A + LAS++LK Sbjct: 79 PQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEAT--LASSKLKAE 136 Query: 118 RYQELRDKNFVSQATLDSKQTAYQSALSGYDQAVASLRNQSNQTGYAVLTADVDGVVTAV 177 RY EL + VS+ D Q+ + + + A+++ + Y + + + G + Sbjct: 137 RYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKS 196 Query: 178 DAEAGQVVAAG-TPVVSVAQAGDKDVVIGIPEDKVDVLRRSADVRVRLWANPKETIVGKI 236 G +V A T ++ Q D + + + + VD+LR +E G+I Sbjct: 197 AVTPGALVTASQTTALTTVQQIDP-IYVDVTQSTVDLLRLR-----------RELASGQI 244 Query: 237 REVSPIADAATRTYTFKIALPDSAKDVRLGMTAYVAFAGKTSGEMIRL 284 R+ + KI L D + R G + + T MI L Sbjct: 245 RK------SGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITL 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 952,037 Number of extensions: 36339 Number of successful extensions: 182 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 8 Length of query: 370 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 283 Effective length of database: 1,207,925 Effective search space: 341842775 Effective search space used: 341842775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)