BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0002.p (375 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 654 0.0 gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 350 1e-97 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 220 2e-58 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 160 2e-40 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 106 3e-24 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 654 bits (1687), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/378 (84%), Positives = 348/378 (92%), Gaps = 3/378 (0%) Query: 1 MYNR---MHIIAASLAIIPAFASAQEVQTVKIGFSSPLTGPQASAGKDNQGGLQMAVERL 57 M+ R ++IIAASLA+IPAFA AQE Q VKIGFSSPLTGPQASAGKDNQGGL MA+ERL Sbjct: 1 MFKRVSGLNIIAASLALIPAFAMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIERL 60 Query: 58 NTQGLVIGGKKIKFEIVAEDDQADPKSGVGVAQKLVDQGVKAIVGPYNSGVTIPASRVYN 117 N Q + +GGKKIKF+++AEDDQADPKSGV VAQKL DQGVKAIVGPYNSGVTIPASRVYN Sbjct: 61 NAQPITVGGKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYN 120 Query: 118 DAGIVTATVASNPKITQQGFATLYRVAASDSQLGGKMALYAAKELKIKTVSVIDDRTAYG 177 DAGIV ATVASNPKITQQGFATL+RVAASDSQLGGKMALYAAKELK K V+VIDDRTAYG Sbjct: 121 DAGIVVATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYG 180 Query: 178 QGLAEEFIKLAQANGIKVVSKDFTNDKATDFTAILTSIKGKKPQAVFIGGYAPQGGPIKR 237 QGLA+EFIK+A+ANGI VVS DFTNDKATDFTAILTSIKGKKP AVF+GGYAPQGGPIKR Sbjct: 181 QGLAQEFIKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKR 240 Query: 238 QMKQLGIDALLMGGDGICSPEMGRLGGDAIGESVYCTQGGTILEKAKEGKAFADDYQKKF 297 QMKQLG+D LMGGDGICSPEMGRLGGDAIGESVYCTQGGT+L+KAKEGK F+D+YQKK+ Sbjct: 241 QMKQLGVDVPLMGGDGICSPEMGRLGGDAIGESVYCTQGGTMLDKAKEGKVFSDEYQKKY 300 Query: 298 GRPAEVYAVSFYDGMMVIAQAMKAANSVEPKVYGPALAKIKYKGVAGQYEFDDKHDLKQS 357 RPAE YAVSFYDGMM+IAQAMK ANSV+PK +GPALAKI YKGVAGQY+FD HDLKQS Sbjct: 301 NRPAETYAVSFYDGMMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHDLKQS 360 Query: 358 PVTIFRFKDGVPVALTSY 375 PVT++RFKDG+PV LTSY Sbjct: 361 PVTVYRFKDGLPVPLTSY 378 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 350 bits (897), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 193/371 (52%), Positives = 241/371 (64%), Gaps = 4/371 (1%) Query: 1 MYNRMHIIAASLAIIPAFASAQEVQ-TVKIGFSSPLTGPQASAGKDNQGGLQMAVERLNT 59 M + II + AI AFA A Q VKI PL+GP A GKDN+ G +MAV+ LN Sbjct: 1 MKLKSAIIPLTAAIGLAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAVDELNA 60 Query: 60 QGLVIGGKKIKFEIVAEDDQADPKSGVGVAQKLVDQGVKAIVGPYNSGVTIPASRVYNDA 119 +G IGGKK+KFE+V EDD +DPK VA KLVDQ V A++G NSG TIPAS++Y+DA Sbjct: 61 KGFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDA 120 Query: 120 GIVTAT-VASNPKITQQGFATLYRVAASDSQLGGKMALYAAKELKIKTVSVIDDRTAYGQ 178 GI + A+NPK TQQGF T +RV A+D+QLG + YA ++L K ++VIDDRTAYGQ Sbjct: 121 GIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQ 180 Query: 179 GLAEEFIKLAQANGIKVVSKDFTNDKATDFTAILTSIKGKKPQAVFIGGYAPQGGPIKRQ 238 G+A+EF K A+A G +V +TNDKATDF AILTS+KGKKP VF GG GGP+ RQ Sbjct: 181 GVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPMLRQ 240 Query: 239 MKQLGIDALLMGGDGICSPEMGRLGGDAIGES-VYCTQGGTILEKAKEG-KAFADDYQKK 296 MKQLGI A MGGDGIC+ + L GD +G+ V C + G + E K+G F Y+KK Sbjct: 241 MKQLGISAKFMGGDGICTGSLPGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAYKKK 300 Query: 297 FGRPAEVYAVSFYDGMMVIAQAMKAANSVEPKVYGPALAKIKYKGVAGQYEFDDKHDLKQ 356 FG A YD M +A AM A S +PK Y P LAKI +KGV G FD K D+K Sbjct: 301 FGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDIKD 360 Query: 357 SPVTIFRFKDG 367 +T++ +K G Sbjct: 361 GSLTLYTYKGG 371 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 220 bits (560), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 122/347 (35%), Positives = 196/347 (56%), Gaps = 4/347 (1%) Query: 22 QEVQTVKIGFSSPLTGPQASAGKDNQGGLQMAVERLNTQGLVIGGKKIKFEIVAEDDQAD 81 QE T+ IGF+ PL+GP GK Q G+Q+A+ N +GL IGGK + ++A+DD+AD Sbjct: 35 QETATLAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRAD 94 Query: 82 PKSGVGVAQKLVDQGVKAIVGPYNSGVTIPASRVYNDAGIVTAT-VASNPKITQQGFATL 140 P + VA+ LV Q V A+VG + SG ++ A+ VY+ AG++ T A + ++T + + Sbjct: 95 PATAEFVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTAMSYPRI 154 Query: 141 YRVAASDSQLGGKMALYAAKELKIKTVSVIDDRTAYGQGLAEEFIKLAQANGIKVVSKDF 200 +R ++ LG A YA +L+ +T+ IDDRT +GQGLAE+F + A G +VV + Sbjct: 155 FRTIPNNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYS 214 Query: 201 TNDKATDFTAILTSIKGKKPQAVFIGGYAPQGGPIKRQMKQLGIDALLMGGDGICS-PEM 259 +DK +DF A L + +P +F GG Q G + + +++L + A LM G P + Sbjct: 215 VSDKTSDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMASPGTLGLPFL 274 Query: 260 GRLGGDAIGESVYCTQGGTILEKAKEGKAFADDYQKKFGRPAEVYAVSFYDGMMVIAQAM 319 R G DA G V + G +K K+F Y + F ++YAV Y+ I Q + Sbjct: 275 MRAGPDANGALVL--EPGPPQDKMPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQGI 332 Query: 320 KAANSVEPKVYGPALAKIKYKGVAGQYEFDDKHDLKQSPVTIFRFKD 366 + A S +P+ A+ +++++GV+G F+++ DL Q T++ KD Sbjct: 333 RQAGSADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMYEVKD 379 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 160 bits (405), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 110/344 (31%), Positives = 173/344 (50%), Gaps = 7/344 (2%) Query: 23 EVQTVKIGFSSPLTGPQASAGKDNQGGLQMAVERLNTQGLVIGGKKIKFEIVAEDDQADP 82 +TVKI + P+TG A G + G A+E++N G G KFE V DD +P Sbjct: 25 HAETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAG---GANGNKFEAVMMDDACEP 81 Query: 83 KSGVGVAQKLVDQGVKAIVGPYNSGVTIPASRVYNDAGIVTAT-VASNPKITQ-QGFATL 140 K V VA K+V QG+K ++G SG TIPAS +Y + G+V T A+ P++T+ + + Sbjct: 82 KQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFI 141 Query: 141 YRVAASDSQLGGKMALYAAKELKIKTVSVIDDRTAYGQGLAEEFIKLAQANGIKVVSKDF 200 +R D Q G A Y ++LK K V+V+ D+ +YGQG+A A + VV + Sbjct: 142 FRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEG 201 Query: 201 TNDKATDFTAILTSIKGKKPQAVFIGGYAPQGGPIKRQMKQLGIDALLMGGDGICSPEMG 260 N +D++AI+T +K + V+ GGY P+ G I RQ ++ GI A+ MG +G+ + ++ Sbjct: 202 INAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVGNKDIT 261 Query: 261 RLGGDAIGESVYCTQGGTILEKAKEGKAFADDYQKKFGRPAEVYAVSFYDGMMVIAQAMK 320 + G A + + A A K+ P + + Y G+ +IA A+ Sbjct: 262 AIAGPASEGMLVTLPADFAADPANAALVKAFAAAKR--DPNGPFQMPAYSGVKIIADAIA 319 Query: 321 AANSVEPKVYGPALAKIKYKGVAGQYEFDDKHDLKQSPVTIFRF 364 A S +P+ + +K G E+D K DLK +F + Sbjct: 320 GAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVVFTW 363 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 110/386 (28%), Positives = 179/386 (46%), Gaps = 31/386 (8%) Query: 2 YNRMHIIAASLAIIPAFASAQEVQTVKIGFSSPLTGPQASAGKDNQGGLQMAVERLNTQG 61 Y +AA+L + ++Q +KIG S P TG A G + G+++AV +N G Sbjct: 3 YKTQAFVAAALMSLAL--TSQAADPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAAG 60 Query: 62 LVIGGKKIKFEIVAEDDQADPKSGVGVAQKLVD-QGVKAIVGPYNSGVTIPASRVYNDAG 120 ++G + +++ DD+A + GV +AQ+L++ + V A VG N+GV + + R Y +A Sbjct: 61 GLLGRQ---IQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAK 117 Query: 121 I-VTATVASNPKITQQGFAT-----LYRVAASDSQLGGKMALYAAKELKIKTVSVIDDRT 174 I V VA+ IT+Q F ++R +A+D +A A + K V+++ D T Sbjct: 118 IPVINNVATGTVITKQ-FEKEKDNYVFRTSAADKIQAKMIADEAVDKQKFTKVAILADST 176 Query: 175 AYGQGLAEEFIKLAQANGIKVVSKDFTNDKATDFTAILTSIKGKKPQAVFIGGYAPQGGP 234 YGQ E+ K+ + V+ + N K D TA L K QAV G P+ Sbjct: 177 NYGQLGREDLEKVLADKKVTPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQ 236 Query: 235 IKRQMKQLGIDALLMGGD----GICSPEMGRLGGDAIGESVYCTQGGTILEKAKEGKAFA 290 I M++L ++G G G+ G A + G T + KAF Sbjct: 237 IANGMEKLNWHVPIIGSWPLSMGNFIDNSGKNGNGARMPQTFIQDGNT-----PKRKAFI 291 Query: 291 DDYQKKFG---RPAEVYAVSFYDGMMVIAQAMKAANSVEPKVYGPALAKI--KYKGVAGQ 345 + YQK + P+ V A YD ++++A A+K A + + AL + K +GV Sbjct: 292 EAYQKAYKVDRMPSAVSAAQGYDSILLLAAAIKQAGTTDGDKVRAALENLNTKVEGVVTT 351 Query: 346 YE----FDDKHDLKQSPVTIFRFKDG 367 Y+ DD +K + V + K+G Sbjct: 352 YDKPFTHDDHEAIKPAMVVMGEVKNG 377 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,187,312 Number of extensions: 52109 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 18 Length of query: 375 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 288 Effective length of database: 1,207,925 Effective search space: 347882400 Effective search space used: 347882400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)