BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0270.p (335 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 618 e-178 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 154 7e-39 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 142 3e-35 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 142 4e-35 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 107 1e-24 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 618 bits (1593), Expect = e-178, Method: Compositional matrix adjust. Identities = 291/332 (87%), Positives = 314/332 (94%) Query: 1 MQYRRLGKSNLKVSALCLGTMMFGDQTPIDEARSIVASAREHGVNFIDTADVYSKGKSEE 60 M YRRLGKSNLKVSALCLG+MMFGDQT + E+ IVASAREHGVNFIDTADVY+KGKSEE Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE 60 Query: 61 MVGKLLQGQRGDWVLASKLGNAMTGAPNQSHYSRHWIVTETDNILRRLDTDYLDVLYMHR 120 +VGKLL GQR +WVLASKLGNAM+G PN+S+YSR WIV ET+NIL RLDTDYLD+LYMHR Sbjct: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHR 120 Query: 121 DYHEENLEEAIQALGDLIRAGKIRGFGVSNFRGWRIAEIARLCEKNNVPLPLVCQPYYNL 180 DY +ENLEEA+QALGDLIR GKIRGFGVSNFRGWRIAEI RLCEKNNVPLPLVCQPYYNL Sbjct: 121 DYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL 180 Query: 181 LNRMPEVEILPACANYGLGVVPYSPIARGVLTGKYKPGQKPAEDTRAGRGDKRILETEFR 240 LNR PEVEILPACA+YGLGVVPYSPIARGVLTGKY+PGQKPA+DTRAGRGDKR++ETEFR Sbjct: 181 LNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFR 240 Query: 241 EESLVIAERLQQHCAARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAVELKIS 300 EESL+IA+RLQQHC ARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDA ELKIS Sbjct: 241 EESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKIS 300 Query: 301 AEEEALIDSLVTPGHSSTPGYNDPSYPFFGRV 332 AEEEAL+DSLVTPGH+ST GYNDP+YPFFGRV Sbjct: 301 AEEEALVDSLVTPGHASTHGYNDPNYPFFGRV 332 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 154 bits (390), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 111/324 (34%), Positives = 169/324 (52%), Gaps = 15/324 (4%) Query: 1 MQYRRLGKSNLKVSALCLGTMM-FGDQTPIDEARSIVASAREHGVNFIDTADVYS--KGK 57 M YRR G+S L + A+ LG FG + R+++ A + GV D A+ Y G Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPAGS 73 Query: 58 SEEMVGKLLQGQ----RGDWVLASKLGNAMTGAP-NQSHYSRHWIVTETDNILRRLDTDY 112 +EE G+ + R + V++SK G M P +R ++ + L+R+ DY Sbjct: 74 AEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIASCEQSLKRMGLDY 133 Query: 113 LDVLYMHRDYHEENLEEAIQALGDLIRAGKIRGFGVSNFRGWRIAEIARLCEKNNVPLPL 172 +D+ Y HR + LEE + AL L R GK G+S++ + A + + VPL L Sbjct: 134 VDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEGVPL-L 192 Query: 173 VCQPYYNLLNRMPEVEILPACANYGLGVVPYSPIARGVLTGKYKPG-QKPAEDTRAGRGD 231 + QP Y++LNR E +LPA G+G + +SP+A+G+LT KY G + A TRA Sbjct: 193 IHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVPEGARVTRAPSLP 252 Query: 232 KRILETEFREESLVIAERLQQHCAARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDY 291 +R+L E++L L + + RG L Q A AWVL ++ ++S + G RT+ Q++D Sbjct: 253 QRMLS----EDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQLDDS 308 Query: 292 YDAV-ELKISAEEEALIDSLVTPG 314 DAV +L + EE A ID T G Sbjct: 309 LDAVNKLDFTQEELASIDKHATDG 332 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 142 bits (358), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 105/351 (29%), Positives = 168/351 (47%), Gaps = 52/351 (14%) Query: 1 MQYRRLGKSNLKVSALCLGTMMFGDQTPIDEARSIVASAREHGVNFIDTADVY------- 53 MQYR LG+++LKVS + LGTM +G+Q +A + + A E G+N ID A++Y Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 54 SKGKSEEMVGKLLQ--GQRGDWVLASKLGNAMTGAPNQSHYSRH-----------WIVTE 100 ++G +E +G L+ G+R ++A+K TG + H RH + Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATK----CTGPARKPHNPRHVRGGINNLDRKGLTEA 116 Query: 101 TDNILRRLDTDYLDVLYMH-----------------RDYHEENLEEAIQALGDLIRAGKI 143 L RL D++D+ +H D +EE + L + ++AGKI Sbjct: 117 LHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKI 176 Query: 144 RGFGVSNFRGWRIAEIARLCEKNNVPLPLVCQPYYNLLNRMPEVEILPACANYGLGVVPY 203 R G+SN W +A+ R E++ + + Q YNLLNR E+ + +G++ Y Sbjct: 177 RTIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAY 236 Query: 204 SPIARGVLTGKYKPGQKPAEDTRAGRGDKRILETEFREESLVIAERLQQHCAA----RGV 259 SP+A G+L+GKY G +PA G + L F + AER A G+ Sbjct: 237 SPLAFGMLSGKYLDGARPA-------GGRLTLFERFSRYTNAQAERATAEYVALARQHGL 289 Query: 260 KLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAVELKISAEEEALIDSL 310 Q A A+V + ++S I G TLAQ+ D+ EL++ + A I+++ Sbjct: 290 DPAQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAI 340 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 142 bits (358), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 104/335 (31%), Positives = 162/335 (48%), Gaps = 28/335 (8%) Query: 4 RRLGKSNLKVSALCLGTMMFGD---QTPIDEARSIVASAREHGVNFIDTADVYSKGKSEE 60 RRLG + VSA+ LG M D Q E+ + + A E G+N +DTAD+Y +EE Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEE 77 Query: 61 MVGKLLQGQRGDWVLASKLG----------NAMTGAPNQSHYSRHWIVTETDNILRRLDT 110 ++G+ ++G+R + +A+K G + G+P +I + L+RL Sbjct: 78 LLGRAIKGRRDKFFIATKFGIRRDPSDPGARGVDGSPA-------YIRQAVEGSLKRLGV 130 Query: 111 DYLDVLYMHRDYHEENLEEAIQALGDLIRAGKIRGFGVSNFRGWRIAEIARLCEKNNVPL 170 + +D+ Y HR +E + L DL++AGKIR G+S A A L + V Sbjct: 131 ETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSE------ASAATLERAHRVHP 184 Query: 171 PLVCQPYYNLLNRMPEVEILPACANYGLGVVPYSPIARGVLTGKYKPGQKPAEDTRAGRG 230 Q Y+L R PE E+L AC G+G V YSP+ RG LTG + + AED R Sbjct: 185 VTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFR-RS 243 Query: 231 DKRILETEFREESLVIAERLQQHCAARGVKLGQFATAWVLANSAISSVIAGPRTLAQMED 290 + R F +L +AE++++ G Q A AWV+A I G + +ED Sbjct: 244 NPRFQGENF-ARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLED 302 Query: 291 YYDAVELKISAEEEALIDSLVTPGHSSTPGYNDPS 325 +V +K++ E+ +D++ G ++ Y D S Sbjct: 303 NAGSVGVKLAPEDLQALDAIFPRGAAAGERYTDAS 337 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 92/318 (28%), Positives = 151/318 (47%), Gaps = 26/318 (8%) Query: 1 MQYRRLGKSNLKVSALCLGTMMFGDQ---TPIDEARSIVASAREHGVNFIDTADVYSKGK 57 M+ RR G++ +VS + G G +A++ + +A + GV+FIDTADVY G+ Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 Query: 58 SEEMVGKLLQGQRGDW-VLASKLGNAMTGAPNQSHYSRHWIVTETDNILRRLDTDYLDVL 116 SE+++ ++L+ + G+ +A+K G ++ + R + D L+ L D LD++ Sbjct: 61 SEQLIAQVLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDLV 120 Query: 117 YMH----RDYHEENLEEAIQALGDLIRAGKIRGFGVSNFRGWRIAEIARLCEKNNVPLPL 172 +H Y++ +E L L+ AGKIR +GVS ++ E + E NV Sbjct: 121 QLHCPPTEVYYQ---QEVFGHLDALVAAGKIRHYGVSV---EKVEEALKAIEYPNVK--- 171 Query: 173 VCQPYYNLLNRMPEVEILPACANYGLGVVPYSPIARGVLTGKYKPGQKPAEDTRAG---R 229 Q +N+ + P L + V+ P+A G+LTGK A D Sbjct: 172 SVQIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRH 231 Query: 230 GDKRILETEFR----EESLVIAERLQQHCAARGVKLGQFATAWVLANSAISSVIAGPRTL 285 G+ + F E +L E+++ +GV + QFA W+L S IS+VI G R + Sbjct: 232 GEAFDVGETFSGVPYEVALAAVEKIRA-LVPQGVPMAQFALRWILMESGISTVIPGARNV 290 Query: 286 AQMEDYYDAVEL-KISAE 302 AQ + A L I+AE Sbjct: 291 AQAQSNSAASALPPITAE 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,089,957 Number of extensions: 46039 Number of successful extensions: 137 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 6 Length of query: 335 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 249 Effective length of database: 1,212,660 Effective search space: 301952340 Effective search space used: 301952340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)