BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0265.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 451 e-128 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 169 3e-43 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 148 5e-37 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 144 9e-36 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 129 3e-31 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 451 bits (1160), Expect = e-128, Method: Compositional matrix adjust. Identities = 231/297 (77%), Positives = 268/297 (90%) Query: 1 VHFKDGAMVKKGDVLFTIDPRPYAAEVARAQAQLAGAEARAAYTASDVARGQRLLGDNAI 60 VHFKDGA+VKKGDVLFTIDPRPY AEVARA+AQLAGAEARA YTASD+ARGQRLL DNAI Sbjct: 90 VHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEARAGYTASDLARGQRLLSDNAI 149 Query: 61 AKRDYEEKQNASREAAANVQAAQAALRSARLNLEYTQIVAPVSGRMSRAEVTVGNIVSVG 120 AKRD+EEKQNASREA+ANV AA+AALR+ARLNLEYT+I APVSGR+SRAEVTVGN+V+ G Sbjct: 150 AKRDFEEKQNASREASANVAAARAALRAARLNLEYTRITAPVSGRISRAEVTVGNVVNPG 209 Query: 121 SATPALSTLVSMSKIYASFDVDEQSFLKYVNPARAKGTSVAVFMGLANEDAYSREGKVGS 180 +A L+TLVS++K+YASFDVDEQSFLKYVNPARA G +V V +GLANED YSR+G+VGS Sbjct: 210 AANAPLTTLVSVAKVYASFDVDEQSFLKYVNPARAAGATVPVELGLANEDGYSRKGRVGS 269 Query: 181 IDNRIDTSSGTIRVRAVFDNADGQLLPGLYARIRLGGGAPRDALLVDEKALGTDQDKRFV 240 +DNR+DT+SGTIRVRA+FDNA+ QL+PGLYAR+RLGGG P A+L+DEKA+GTDQDKRFV Sbjct: 270 VDNRLDTASGTIRVRAIFDNANSQLVPGLYARVRLGGGEPHQAVLIDEKAIGTDQDKRFV 329 Query: 241 LVLDKDNHATYREVRVGANQDGLRVIESGLKSGERVVVNGLQRVRPGDTVAPNLVPM 297 +V DKDNHA YR+V VGA DGL VIE GL+ GER+VVNGLQR+RPGD + P +VPM Sbjct: 330 MVADKDNHANYRQVVVGAGHDGLVVIEKGLQPGERIVVNGLQRIRPGDAITPTVVPM 386 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 169 bits (428), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 109/297 (36%), Positives = 163/297 (54%), Gaps = 9/297 (3%) Query: 3 FKDGAMVKKGDVLFTIDPRPYAAEVARAQAQLAGAEARAAYTASDVARGQRLLGDNAIAK 62 FK+G+ VK+G VLF IDP Y A + AQA LA A+A T R + L+ NA++K Sbjct: 80 FKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSK 139 Query: 63 RDYEEKQNASREAAANVQAAQAALRSARLNLEYTQIVAPVSGRMSRAEVTVGNIVSVGSA 122 ++Y+ A ++A A+V A +AA+++A LNL Y + +P+SGR+ RA VT G +V G A Sbjct: 140 QEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDA 199 Query: 123 TPALSTLVSMSKIYASFDVDEQSFLKYVNPARAKG-------TSVAVFMGLANEDAYSRE 175 T L+ + + +Y + LK + A A G +V + L N AY Sbjct: 200 T-QLALVQQLDPVYVNVTQSSADLLK-LQQAMAAGQLKSVGDNKASVTLELENGQAYPLP 257 Query: 176 GKVGSIDNRIDTSSGTIRVRAVFDNADGQLLPGLYARIRLGGGAPRDALLVDEKALGTDQ 235 GK+ D +D ++G++ +RA F N LLPG Y R ++ ALLV ++A+ D Sbjct: 258 GKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRDA 317 Query: 236 DKRFVLVLDKDNHATYREVRVGANQDGLRVIESGLKSGERVVVNGLQRVRPGDTVAP 292 V+V++ +N R V+ G + + GLK G+RV+V GLQ+VRPG TV P Sbjct: 318 GGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATVKP 374 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 148 bits (374), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 105/293 (35%), Positives = 155/293 (52%), Gaps = 8/293 (2%) Query: 3 FKDGAMVKKGDVLFTIDPRPYAAEVARAQAQLAGAEARAAYTASDVARGQRLLGDNAIAK 62 FK+G+ VK G L+ IDP Y A A+A L AEA A + R L +A++K Sbjct: 90 FKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLKAERYAELNKIDAVSK 149 Query: 63 RDYEEKQNASREAAANVQAAQAALRSARLNLEYTQIVAPVSGRMSRAEVTVGNIVSVGSA 122 +D ++ Q+ R+ A+V+ +AA+++A +NL+YT+I++P+SGR+ ++ VT G +V+ S Sbjct: 150 QDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSAVTPGALVT-ASQ 208 Query: 123 TPALSTLVSMSKIYASFDVDEQSFLKY-----VNPARAKGTSVA-VFMGLANEDAYSREG 176 T AL+T+ + IY L+ R G + A V + L + Y R G Sbjct: 209 TTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGENAAQVKIVLEDGSTYERPG 268 Query: 177 KVGSIDNRIDTSSGTIRVRAVFDNADGQLLPGLYARIRLGGGAPRDALLVDEKALGTDQD 236 K+ +DT +G I +RAV N DG LLPG Y R L G ALL +KA+ DQ Sbjct: 269 KLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDDHALLAPQKAVSRDQT 328 Query: 237 -KRFVLVLDKDNHATYREVRVGANQDGLRVIESGLKSGERVVVNGLQRVRPGD 288 LVL D R V V + GLK G++++V+GLQ+VR GD Sbjct: 329 GAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLKEGDKLIVDGLQKVRTGD 381 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 144 bits (363), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 96/297 (32%), Positives = 153/297 (51%), Gaps = 16/297 (5%) Query: 1 VHFKDGAMVKKGDVLFTIDPRPYAAEVARAQAQLAGAEARAAYTASDVARGQRLLGDNAI 60 +H+K+G +VK GD+L IDPR A + +A+ QLA A A D+ R Q LL ++I Sbjct: 73 LHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLAQDSI 132 Query: 61 AKRDYEEKQNASREAAANVQAAQAALRSARLNLEYTQIVAPVSGRMSRAEVTVGNIVSVG 120 A + + + ++ V+A + + SARL L +T++ AP+SGR+ + VGN V+ Sbjct: 133 ASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGLRQADVGNNVTT- 191 Query: 121 SATPALSTLVSMSKIYASFDVDEQSFLKYVNPARAKGTSVAVFMGLANEDAYSREGK--- 177 S T L + + I A F + E + K + ++ A A+ RE K Sbjct: 192 SDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPA--------QAWDREQKNKL 243 Query: 178 ----VGSIDNRIDTSSGTIRVRAVFDNADGQLLPGLYARIRLGGGAPRDALLVDEKALGT 233 + +IDN +D ++GT++++A F N D L P + IRL + A ++ A+ Sbjct: 244 ADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTAAIQR 303 Query: 234 DQDKRFVLVLDKDNHATYREVRVGANQDGLRVIESGLKSGERVVVNGLQRVRPGDTV 290 FV V+ D+ T R V+ G QD L VI G+ +GE VV++G+ R+R G V Sbjct: 304 GSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRLREGAKV 360 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 129 bits (324), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 97/304 (31%), Positives = 159/304 (52%), Gaps = 15/304 (4%) Query: 3 FKDGAMVKKGDVLFTIDPRPYAAEVARAQAQLAGAEARAAYTASDVARGQRLLGDNAIAK 62 F++G+ VK G VL+ I+P Y A + A LA AEA +R + L+ A+++ Sbjct: 82 FREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELVAIKAVSQ 141 Query: 63 RDYEEKQNASREAAANVQAAQAALRSARLNLEYTQIVAPVSGRMSRAEVTVGNIVSVGSA 122 +DY++ + EA A+V +A+A ++++R+NL+Y ++ AP+SGR+ ++ VT G +V+ Sbjct: 142 QDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGALVTANQT 201 Query: 123 TPALSTLVSMSKIYASFDVDEQSFLKYVNPARA--------KGTSVAVFMGLANEDAYSR 174 T AL+T+ + IY DV QS ++N A G V + L N Y++ Sbjct: 202 T-ALTTIQQLDPIY--VDV-TQSSTTWLNLKEALANGQLEKSGNGAKVRLVLENGRTYAQ 257 Query: 175 EGKVGSIDNRIDTSSGTIRVRAVFDNADGQLLPGLYARIRLGGGAPRDALLVDEKALGTD 234 EG + D ++ +G I +RA+F N LLPG+Y R L G + LLV ++A+ D Sbjct: 258 EGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAVSRD 317 Query: 235 -QDKRFVLVLDKDNHATYREVRVGANQDGLRVIESGLKSGERVVVNGLQRVRPGDTVAPN 293 K V++ R + ++ SGLK G+++VV+G QR G V Sbjct: 318 GAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAGAKV--K 375 Query: 294 LVPM 297 +VP Sbjct: 376 VVPW 379 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 808,532 Number of extensions: 30887 Number of successful extensions: 159 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 148 Number of HSP's successfully gapped: 12 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)