BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0264.p (331 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump pr... 446 e-127 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 222 3e-59 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 222 3e-59 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 216 2e-57 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 210 1e-55 >gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1071 Score = 446 bits (1148), Expect = e-127, Method: Compositional matrix adjust. Identities = 242/270 (89%), Positives = 257/270 (95%) Query: 1 MNISKFFIDRPIFAGVLSILLLLAGVLAMFQLPISEYPEVVPPSVVVRASYPGANPKVIA 60 MNISKFFIDRPIFAGVLS+LLLL GVLAM QLPISEYPEVVPPSVVVRA YPGANPKVIA Sbjct: 1 MNISKFFIDRPIFAGVLSVLLLLGGVLAMLQLPISEYPEVVPPSVVVRAQYPGANPKVIA 60 Query: 61 ETVASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVAQALPRL 120 ETVASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRV+QALPRL Sbjct: 61 ETVASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVSQALPRL 120 Query: 121 PEDVQRLGVTTIKSSPTLTMVVHLISPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLF 180 PEDVQRLGVTT+KSSPTLTMVVHL SPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLF Sbjct: 121 PEDVQRLGVTTLKSSPTLTMVVHLTSPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLF 180 Query: 181 GSGNYSMRVWLDPNKVSQRGLTASDVIKSIREQNVQVAAGVIGASPSSPDVPVQLSVNAQ 240 GSGNY+MRVWLDPNKV+QRGLTA+DV+++IREQNVQVAAGVIGASPSSP+ P+QLSVNAQ Sbjct: 181 GSGNYAMRVWLDPNKVAQRGLTATDVVRAIREQNVQVAAGVIGASPSSPETPLQLSVNAQ 240 Query: 241 GRLKSEEEFREIILKSDAGGAITKLGRRRR 270 GRLK+E EFR+IILK+ GA T+LG R Sbjct: 241 GRLKTEGEFRDIILKASPDGATTRLGDVAR 270 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 222 bits (566), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 114/289 (39%), Positives = 187/289 (64%), Gaps = 1/289 (0%) Query: 3 ISKFFIDRPIFAGVLSILLLLAGVLAMFQLPISEYPEVVPPSVVVRASYPGANPKVIAET 62 +++FFIDRPIFA VL+I+++LAG++++ LPI++YP + PP++ + A+YPGA+ K + +T Sbjct: 1 MARFFIDRPIFAWVLAIIVMLAGIVSVLTLPIAQYPAIAPPAIAITANYPGASAKTLEDT 60 Query: 63 VASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVAQALPRLPE 122 V +E+++ G++ + YM S + S G++TIT+ F G +PD AQ VQN+++ A P LP Sbjct: 61 VTQIIEQKMKGLDRLSYMASTSESSGSVTITLTFENGTNPDTAQVQVQNKLSLATPLLPS 120 Query: 123 DVQRLGVTTIKSSPTLTMVVHLISPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLFGS 182 +VQ+ G+T KS+ V+ S D + L +Y NV+D ++R++GVG+ LFGS Sbjct: 121 EVQQQGITVTKSATNFLNVLAFTSEDGSMSGSDLSDYVAANVQDAISRVEGVGDTTLFGS 180 Query: 183 GNYSMRVWLDPNKVSQRGLTASDVIKSIREQNVQVAAGVIGASPSSPDVPVQLSVNAQGR 242 Y+MR+WLDPNK++ GLT DV +I+ QN QV++G +G P++ + + ++ AQ R Sbjct: 181 -QYAMRIWLDPNKLNSYGLTPLDVKNAIQAQNAQVSSGQLGGLPAAENQQINATITAQTR 239 Query: 243 LKSEEEFREIILKSDAGGAITKLGRRRRPRRAGRVRIRSARAAGQQACG 291 L++ ++F+ I+L++ GGA KL R R G+ A G Sbjct: 240 LRTADQFQNIVLRTLTGGAQVKLRDVARIELGSESYTSVGRYNGKPAAG 288 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 222 bits (566), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 110/262 (41%), Positives = 173/262 (66%), Gaps = 1/262 (0%) Query: 5 KFFIDRPIFAGVLSILLLLAGVLAMFQLPISEYPEVVPPSVVVRASYPGANPKVIAETVA 64 +FFIDRPIFA V+++ ++L GV+AM QLPI+ YP V PP+V + A+YPGA P+ + + V Sbjct: 3 RFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDGVI 62 Query: 65 SPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVAQALPRLPEDV 124 S +E +++GV+N+LY +S A++ G I+V F+ G DP+ AQ VQN++ RLP+ V Sbjct: 63 SLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQTV 122 Query: 125 QRLGVTTIKSSPTLTMVVHLISPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLFGSGN 184 ++ GVT ++ + ++V L S D RYD L +Y N+ + L R++GVG V LFG+ Sbjct: 123 RQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGA-E 181 Query: 185 YSMRVWLDPNKVSQRGLTASDVIKSIREQNVQVAAGVIGASPSSPDVPVQLSVNAQGRLK 244 +MR+WLDP K++ GLT +D+ +I +QN Q+A G +G +P+ + + + QG+L+ Sbjct: 182 QAMRIWLDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQLQ 241 Query: 245 SEEEFREIILKSDAGGAITKLG 266 S +F I+LK+ A G+ LG Sbjct: 242 SPAQFAAIVLKAKADGSRVLLG 263 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 216 bits (551), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 107/286 (37%), Positives = 185/286 (64%), Gaps = 5/286 (1%) Query: 3 ISKFFIDRPIFAGVLSILLLLAGVLAMFQLPISEYPEVVPPSVVVRASYPGANPKVIAET 62 +++FFIDRPIFA V++I+++L G++++ L + +YP++ PP V ++A+Y GA+ K + ++ Sbjct: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 Query: 63 VASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVAQALPRLPE 122 V +E+Q+ G++N+ YM + ++S G+ T+++ F G +PD AQ VQN++ Q RLP+ Sbjct: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVSLTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 Query: 123 DVQRLGVTTIKSSPTLTMVVHLISPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLFGS 182 VQ GVT K+S + MV+ L S D R + + ++ + D+++R++GVG+V FG+ Sbjct: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 Query: 183 GNYSMRVWLDPNKVSQRGLTASDVIKSIREQNVQVAAGVIGASPSSPDVPVQLSVNAQGR 242 NY+MR+W+DP K+ + L SD+ +++ QN +V+AG IGA P+ P + ++ A+ + Sbjct: 181 -NYAMRIWIDPAKLQKYALMPSDITSALQAQNTEVSAGEIGALPAQPGQQINATITARSK 239 Query: 243 LKSEEEFREIILKSDAGGAITKLGRRRRPRRAGRVRIRSARAAGQQ 288 L + +EFR II+KS G++ KL R G S + A QQ Sbjct: 240 LTTPDEFRNIIVKSSVDGSVVKLSDVARVELGG----ESYQVASQQ 281 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 210 bits (535), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 112/285 (39%), Positives = 172/285 (60%), Gaps = 1/285 (0%) Query: 3 ISKFFIDRPIFAGVLSILLLLAGVLAMFQLPISEYPEVVPPSVVVRASYPGANPKVIAET 62 + KFFIDRP+FA VL+I ++LAG LA+ LP+S+YP + PP++VV A+YPGA + + ++ Sbjct: 1 MGKFFIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDS 60 Query: 63 VASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVAQALPRLPE 122 V S +E+++NG + + Y++SQ+ ++G + IT+ F +PD A VQNR+ + RLP Sbjct: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 Query: 123 DVQRLGVTTIKSSPTLTMVVHLISPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLFGS 182 V + GV KS + + L S D + D L +Y NV + + R+ GVG+ LFG+ Sbjct: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 Query: 183 GNYSMRVWLDPNKVSQRGLTASDVIKSIREQNVQVAAGVIGASPSSPDVPVQLSVNAQGR 242 +MR+W+DPNK+ LT +DV +I+ QN VA G +GA PS + +V G+ Sbjct: 181 -ERAMRIWIDPNKLIGLNLTPADVTAAIQAQNALVAGGTLGALPSPGTQQIAATVVVNGQ 239 Query: 243 LKSEEEFREIILKSDAGGAITKLGRRRRPRRAGRVRIRSARAAGQ 287 L S E+F IIL+++A GA +L R G+ S R G+ Sbjct: 240 LTSPEQFGNIILRANANGATVRLRDVARVEVGGQSYDTSGRLDGK 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 850,521 Number of extensions: 30898 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 10 Length of query: 331 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 245 Effective length of database: 1,212,660 Effective search space: 297101700 Effective search space used: 297101700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)