BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0262.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 551 e-158 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 220 1e-58 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 194 8e-51 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 192 5e-50 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 187 9e-49 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 551 bits (1421), Expect = e-158, Method: Compositional matrix adjust. Identities = 294/372 (79%), Positives = 332/372 (89%) Query: 15 KLLKPLRISSALLAAMLVLAGCSLAPTYERPAVNTSVAYKEAADESANAKAASDWKTAQP 74 +LL+P+RI SALLAA+LVLAGCS+APTYERP+V+T A+KEAA + + S WKTAQP Sbjct: 10 RLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQP 69 Query: 75 AEDLARGEWWKIFNDEGLNALEVQALEANQNLKAAAARLSQSRALRQDARSGLFPQVNAG 134 AED+ARGEWWKIF DE LNALE A +ANQ+LKAAAARL Q+RAL +DAR+G FPQV+AG Sbjct: 70 AEDIARGEWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAG 129 Query: 135 FGATRQRPSPASQGLAPDANTTPNTLWRAQLGVSYEADLFGRVASTVDAATADAQRSEAL 194 GATRQRPSPASQGL DA+T PNTL+RAQ+ VSYE DLFGRVASTVDAATADAQRSEAL Sbjct: 130 VGATRQRPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEAL 189 Query: 195 FHSVRLALQADVAQQYFLLRELDAEQELYSGTVDLRTQSLKLVQRRFNEGDISEVDVARA 254 FHSV LALQADVAQQYFL+RELDA LY GTVDLR +SL+LVQRRF+EGDISEVDVARA Sbjct: 190 FHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARA 249 Query: 255 KSELASAQSESFGIARQRANAEHALAILLGKTPAEFDFKPQPLQRLSITIPAGLPSDLLE 314 ++ELASAQSE+ GIARQRA AEHALAILLG+TPAEF PQPL RL++T+P GLPS LLE Sbjct: 250 RAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVTVPPGLPSALLE 309 Query: 315 RRPDIAAAERAMAAANARVGVARSAWFPRLDLTGGLGYESAKLGDLFNWSSRTFLLGPLV 374 RRPDIAAAERAMAAANARVGVA+SA+FP L+LTGGLGYESA+LGDLFNWSSRTFLLGPLV Sbjct: 310 RRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLV 369 Query: 375 GTALTLPIFDGG 386 GTAL++P+FDGG Sbjct: 370 GTALSMPLFDGG 381 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 220 bits (561), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 153/375 (40%), Positives = 219/375 (58%), Gaps = 22/375 (5%) Query: 17 LKPLRISSALLAAMLVLAGCSLAPTYERPAVNTSVA-YKEAADESANAKAASDWKTAQPA 75 LKPL A+L A L LA CS+ P Y RP ++T A +KE +WK AQP Sbjct: 9 LKPLL---AMLVASLALAACSVGPDYVRPQMDTGPAIFKEG----------QNWKPAQPR 55 Query: 76 EDLARGEWWKIFNDEGLNALEVQALEANQNLKAAAARLSQSRALRQDARSGLFPQVNAGF 135 + +A G+WW++F D LNAL Q +NQNL A A + AL Q +RS P ++A Sbjct: 56 DLVADGKWWEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASA 115 Query: 136 GATRQRPSPASQGLAPDANTTPNTLWRAQ----LGVSYEADLFGRVASTVDAATADAQRS 191 GA+R A + A+ + R L ++EAD++G+V V+++ A AQ S Sbjct: 116 GASRGSSGSAGASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQAS 175 Query: 192 EALFHSVRLALQADVAQQYFLLRELDAEQELYSGTVDLRTQSLKLVQRRFNEGDISEVDV 251 A + RL+ QA VAQ Y LR LDA+Q+L TV +SL++ Q ++ G +++ DV Sbjct: 176 AADLAAARLSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDV 235 Query: 252 ARAKSELASAQSESFGIARQRANAEHALAILLGKTPAEFDFKPQPLQRLSITIPAGLPSD 311 +A+++L SAQ+ + RA EHA+A+L+GKTPA+F PL ++ +P G+PS Sbjct: 236 IQAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSM 295 Query: 312 LLERRPDIAAAERAMAAANARVGVARSAWFPRLDLTGGLGYESAKLGDLFNWSSRTFLLG 371 LLERRPD+AAAER +A+ANA++GVA++A+FP L L+ GY+S+ D F R + LG Sbjct: 296 LLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLG 355 Query: 372 PLVGTALTLPIFDGG 386 P L IFDGG Sbjct: 356 P----QLAQTIFDGG 366 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 194 bits (494), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 142/378 (37%), Positives = 198/378 (52%), Gaps = 41/378 (10%) Query: 20 LRISSALLAAMLVLAGCSLAPTYERPAVNTSVAYKEAADESANAKAASDWKTAQPAEDLA 79 L + +AL+ A VL GCSLAPTYERP +Y PA+ Sbjct: 13 LHMCAALVMAG-VLGGCSLAPTYERPQAPVESSY--------------------PADAAG 51 Query: 80 RGE-------WWKIFNDEGLNALEVQALEANQNLKAAAARLSQSRALRQDARSGLFPQVN 132 R E W + F D+ L L ALE N++L+ A + ++RA Q AR+ L P +N Sbjct: 52 RAELQAVNLGWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLN 111 Query: 133 AGFGATRQRPSPASQGLAPDANTTPNTLWRAQLGV-SYEADLFGRVASTVDAATADAQRS 191 A R R + ASQ L T + ++ L V +YE D FGRV + DAA A + Sbjct: 112 LSGNAARTR-TAASQSLT--GQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLST 168 Query: 192 EALFHSVRLALQADVAQQYFLLRELDAEQELYSGTVDLRTQSLKLVQRRFNEGDISEVDV 251 E S +++L A VAQ Y R + +L T+ R +L L Q+R + G + +D Sbjct: 169 EEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDF 228 Query: 252 ARAKSELASAQSESFGIARQRANAEHALAILLGKTPAEFDFKPQPL---QRLSITIPAGL 308 ++ + SA+ + RQRA AE+AL +L+GK E P L +++ IPAGL Sbjct: 229 RLTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGEL--PPAQLLSDEKIVTDIPAGL 286 Query: 309 PSDLLERRPDIAAAERAMAAANARVGVARSAWFPRLDLTGGLGYESAKLGDLFNWSSRTF 368 PSDLL RPDI +AE+++ AANA +G AR+A+FPR+ LTG G S+ LG LF S + Sbjct: 287 PSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAW 346 Query: 369 LLGPLVGTALTLPIFDGG 386 GP +L+LPIFD G Sbjct: 347 SFGP----SLSLPIFDFG 360 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 192 bits (487), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 149/385 (38%), Positives = 213/385 (55%), Gaps = 38/385 (9%) Query: 19 PLRISSALLAAML--VLAGCSLAPTYERPAVNTSVAYKEAADESANAKAASDWKTAQPAE 76 P R + + A +L +L GC++ P Y RPA++ AYKE WK A P + Sbjct: 28 PFRATKGMTALVLAALLGGCAVGPDYARPALDLPQAYKETGP----------WKLAAPRQ 77 Query: 77 DLARGEWWKIFNDEGLNALEVQALEANQNLKAAAARLSQSRALRQDARSGLFPQVNAGFG 136 WW+ + D LNAL QA ANQ ++ AAA+ Q++A + AR+ L+P + A G Sbjct: 78 IDDHQPWWEAYGDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAG 137 Query: 137 ATRQRPSPASQGLAPDANTTPNTLWRAQLGVSYEADLFGRVASTVDAATADAQRSEALFH 196 A+R + + + G+ N+ + L S+EADL+GR+ +A A+AQ S A Sbjct: 138 ASRAQTN--TNGVQRLGNS-----YSVGLNASWEADLWGRIRRGAEAGEANAQASAASLA 190 Query: 197 SVRLALQADVAQQYFLLRELDAEQELYSGTVDLRTQSLKLVQRRFNEGDISEVDVARAKS 256 + RL++QA +AQ Y LR D +++LY TV T+SL+L ++ G DVA+A++ Sbjct: 191 AARLSIQATLAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAET 250 Query: 257 ELASAQSESFGIARQRANAEHALAILLGKTPAEFDFKPQP---------------LQRLS 301 +L +AQ++ + R EHA+A+L+GK PA+F P LQ Sbjct: 251 QLRAAQAQLIDLDDTRNQLEHAIAMLIGKAPAQFSLPALPPAAASDSAIDANAARLQGSL 310 Query: 302 ITIPAGLPSDLLERRPDIAAAERAMAAANARVGVARSAWFPRLDLTGGLGYESAKLGDLF 361 IP GLPS+LLERRPDIA AER AAANA +GVAR+A+FP L L+ GY S +LF Sbjct: 311 PQIPVGLPSELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLF 370 Query: 362 NWSSRTFLLGPLVGTALTLPIFDGG 386 N SR + L G+AL +FDGG Sbjct: 371 NTPSRVWSL----GSALAESLFDGG 391 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 187 bits (476), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 145/357 (40%), Positives = 195/357 (54%), Gaps = 22/357 (6%) Query: 35 GCSLAPTYERPAVNTSVAYKEAADESANAKAASDWKTAQPAEDLARGEWWKIFNDEGLNA 94 GC+ P Y RP V+ A+ A W++ QPA+ RG WW+ F D LNA Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAP----------WRSMQPADATPRGPWWERFGDAQLNA 67 Query: 95 LEVQALEANQNLKAAAARLSQSRALRQDARSGLFPQVNAGFGATRQRPS---PASQGLAP 151 L+ QAL NQ L A ARL+Q+RA + PQVN A R R S P + +P Sbjct: 68 LQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNYNSP 127 Query: 152 DANTTPNTLWRAQLG--VSYEADLFGRVASTVDAATADAQRSEALFHSVRLALQADVAQQ 209 + +T N LG VSYE D FGRV +V+AA A A++S A + RL L A++A Sbjct: 128 NFSTVQNDF---ALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATN 184 Query: 210 YFLLRELDAEQELYSGTVDLRTQSLKLVQRRFNEGDISEVDVARAKSELASAQSESFGIA 269 YF LRELD E ++ + L+ Q+L+L R + G S +D+A+ ++ L S ++ + Sbjct: 185 YFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLG 244 Query: 270 RQRANAEHALAILLGKTPAEFDFKPQPLQRLSITIPAGLPSDLLERRPDIAAAERAMAAA 329 RQRA EHA+A L G F P IP G+PSD+LERRPDIAAAERAMAAA Sbjct: 245 RQRAQYEHAIATLTGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAA 304 Query: 330 NARVGVARSAWFPRLDLTGGLGYESAKLGDLFNWSSRTFLLGPLVGTALTLPIFDGG 386 NA++GVA +A++P L G +S LFN S + L G + + +FDGG Sbjct: 305 NAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSL----GVSASQSLFDGG 357 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 942,417 Number of extensions: 32242 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 18 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)