BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0256.p (541 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 575 e-165 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 483 e-137 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 454 e-128 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 452 e-128 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 396 e-111 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 575 bits (1483), Expect = e-165, Method: Compositional matrix adjust. Identities = 331/518 (63%), Positives = 403/518 (77%), Gaps = 7/518 (1%) Query: 1 MGRMKIGKRLTLGFAAIMLLSIVITAIGIWRLQAVSDVTRAMVEHSARKERLISSWYGSL 60 +G M IG+RLTLGFA I+ SI+I IG+WRL V+ TR M+ + ERL++ WY +L Sbjct: 4 IGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTNL 63 Query: 61 RAGILRXKVRTIAISRSADPTLKTYFIDEAKASTNNSTRLQKEVGELLSSDEEKSLFQNI 120 AGI R TIAI++S+DP L YF E AS+ +S QK+V LLS+ EEK+LFQ I Sbjct: 64 VAGIRR----TIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKI 119 Query: 121 GERRKVYLASHYRILDLKSAGNVEEGNRLLDQEFVPLARSYQDSMLKLLELEQRDIDKTT 180 GE RK+YL+S I K+AGN EE R+LDQ FVP A +YQD+M KL++ +++++D T Sbjct: 120 GELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTA 179 Query: 181 QQIDDIAAGSRRLLLILEGLSLLLGCFCAWYLTVGITRPINAALGISRRVAAGDLTTQVS 240 ++ID IAA SR L+L+LEGL LLLG A YLT+GIT+P++ A+ +SR VA GDL+++++ Sbjct: 180 REIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSEIA 239 Query: 241 GGDGKSADEVGQLLSSLNDMNGSLRNIVSNVRQGTNTIAVASAEISVGNHDLSARTEQQA 300 S DE GQLL SL DMN SLR IVSNVR GT+TI+ AS+EI+ GN DLS+RTEQQA Sbjct: 240 V---TSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQA 296 Query: 301 SALEETASAMEELISTVRQNADNADTASRLAAAASGIAVEGGEVVGRVVTTMSAINGSSR 360 S+L ETASAMEELISTVRQNADNA AS+LA +AS +A +GG VV +VV TM AIN SSR Sbjct: 297 SSLAETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSR 356 Query: 361 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSATAAKEIKL 420 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA+AAKEIK Sbjct: 357 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKE 416 Query: 421 LIDDSVKTVGEGGVLVEQAGVTMERVVGSVRKVAEIVGEISLASREQASGIEQVNHAISQ 480 LI+DSV V +G LVEQAG TM+ VV SVR+V +IV EIS AS EQ GIEQ+N AI+Q Sbjct: 417 LINDSVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQ 476 Query: 481 MDEMTQQNAALVEQAAAAASSMQTQAASLAQLVSVFSL 518 MD++TQQNAALVE+AAAAA+SMQTQA L Q+VSVF L Sbjct: 477 MDQVTQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRL 514 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 483 bits (1243), Expect = e-137, Method: Compositional matrix adjust. Identities = 283/536 (52%), Positives = 364/536 (67%), Gaps = 7/536 (1%) Query: 1 MGRMKIGKRLTLGFAAIMLLSIVITAIGIWRLQAVSDVTRAMVEHSARKERLISSWYGSL 60 M +KIG RL +GF A+++L V+ A+G+WRL ++ D T M + +KER W+ Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWH--- 57 Query: 61 RAGILRXKVRTIAISRSADPTLKTYFIDEAKASTNNSTRLQKEVGELLSSDEEKSLFQNI 120 A I VRT A+ +S D + YF + A + ++LQKEV ++ EEK LF + Sbjct: 58 -AAIKENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEV 116 Query: 121 GERRKVYLASHYRILDLKSAGNVEEGNRLLDQEFVPLARSYQDSMLKLLELEQRDIDKTT 180 G+ R Y A+ I D+K+AG+ + D V + Y D +L+ + +++ ID+T Sbjct: 117 GKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTA 176 Query: 181 QQIDDIAAGSRRLLLILEGLSLLLGCFCAWYLTVGITRPINAALGISRRVAAGDLTTQVS 240 I+ R +LL L + ++LG AW LT ITRP+N A+ I+ VAAGDLT+++ Sbjct: 177 AAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRI- 235 Query: 241 GGDGKSADEVGQLLSSLNDMNGSLRNIVSNVRQGTNTIAVASAEISVGNHDLSARTEQQA 300 D S DE G+LL++L MNG+L +IV VR GT TI AS EI+ GN DLSARTE QA Sbjct: 236 --DSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQA 293 Query: 301 SALEETASAMEELISTVRQNADNADTASRLAAAASGIAVEGGEVVGRVVTTMSAINGSSR 360 +LEETASAME+L STV+QNADNA A+ LAA+AS +A +GG VV +VV TM AI+ SS+ Sbjct: 294 GSLEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQ 353 Query: 361 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSATAAKEIKL 420 KI DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AAKEIK Sbjct: 354 KIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQ 413 Query: 421 LIDDSVKTVGEGGVLVEQAGVTMERVVGSVRKVAEIVGEISLASREQASGIEQVNHAISQ 480 LIDDSV+ V +G LV +AG TM+ VV SV +V +V EI+LAS+EQ+ GIEQVN AI+ Sbjct: 414 LIDDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITH 473 Query: 481 MDEMTQQNAALVEQAAAAASSMQTQAASLAQLVSVFSLDHGARPAASLGSQSLAPA 536 +DE TQQNAALVEQAAAAA SMQ QAASL VSVF L+ A+ A + +P+ Sbjct: 474 IDETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAVAVQRGTSPS 529 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 454 bits (1167), Expect = e-128, Method: Compositional matrix adjust. Identities = 286/515 (55%), Positives = 364/515 (70%), Gaps = 4/515 (0%) Query: 4 MKIGKRLTLGFAAIMLLSIVITAIGIWRLQAVSDVTRAMVEHSARKERLISSWYGSLRAG 63 MKIG+RL LGFA I+ + V+ A+ IWRLQ ++ T M+ KERL+S WY ++ Sbjct: 7 MKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWYRTIHTS 66 Query: 64 ILRXKVRTIAISRSADPTLKTYFIDEAKASTNNSTRLQKEVGELLSSDEEKSLFQNIGER 123 + R T AI++S+DP+L +F D+A A++ ST QK + L+ SD+EK++F +GE Sbjct: 67 VRR----TTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLGEV 122 Query: 124 RKVYLASHYRILDLKSAGNVEEGNRLLDQEFVPLARSYQDSMLKLLELEQRDIDKTTQQI 183 RK Y+ I K+ G EE ++L F + Y D + +LL L++ +ID+ I Sbjct: 123 RKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAAHI 182 Query: 184 DDIAAGSRRLLLILEGLSLLLGCFCAWYLTVGITRPINAALGISRRVAAGDLTTQVSGGD 243 + SR L+L L L ++LG A L +GITRP+ A+ ++ VAAGDLT ++ Sbjct: 183 QTVYRESRNLMLALAFLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDERL 242 Query: 244 GKSADEVGQLLSSLNDMNGSLRNIVSNVRQGTNTIAVASAEISVGNHDLSARTEQQASAL 303 DE G+L+ +L+ M G+L IV VR GT+ IA AS EI+ GN DLSARTE+QA AL Sbjct: 243 AARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAGAL 302 Query: 304 EETASAMEELISTVRQNADNADTASRLAAAASGIAVEGGEVVGRVVTTMSAINGSSRKIV 363 EETASAMEEL STV QNADNA A++LAA+AS +A +GGEVV +V++TM +IN SSRKIV Sbjct: 303 EETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRKIV 362 Query: 364 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSATAAKEIKLLID 423 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AAKEIK LID Sbjct: 363 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKALID 422 Query: 424 DSVKTVGEGGVLVEQAGVTMERVVGSVRKVAEIVGEISLASREQASGIEQVNHAISQMDE 483 DSV VG G LV +AG TM+ VV SVR+VA+IV EIS AS EQ+ GI QVN AI+ MD+ Sbjct: 423 DSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDMDQ 482 Query: 484 MTQQNAALVEQAAAAASSMQTQAASLAQLVSVFSL 518 TQQNAALVE+AAAAA +MQ QAA L+++VS F L Sbjct: 483 TTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKL 517 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 452 bits (1164), Expect = e-128, Method: Compositional matrix adjust. Identities = 272/515 (52%), Positives = 361/515 (70%), Gaps = 7/515 (1%) Query: 4 MKIGKRLTLGFAAIMLLSIVITAIGIWRLQAVSDVTRAMVEHSARKERLISSWYGSLRAG 63 ++IG RL GFA +++ S+V+ A+GIWRL+ V+ T+ M++ + ERL+S W L Sbjct: 5 IRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTLLLIA 64 Query: 64 ILRXKVRTIAISRSADPTLKTYFIDEAKASTNNSTRLQKEVGELLSSDEEKSLFQNIGER 123 I R T + +S DP L+ + EA AS+ S + K+V +LL+S+EEK +F I Sbjct: 65 IQR----TTTVVKSHDPELEAFLAKEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVA 120 Query: 124 RKVYLASHYRILDLKSAGNVEEGNRLLDQEFVPLARSYQDSMLKLLELEQRDIDKTTQQI 183 RK +L +I K G+ + L +++V +A+S QD+M LL+ E+ ID+ + QI Sbjct: 121 RKQFLTIRDQIYAAKKQGDAAQVEELYQKQYVGIAKSTQDAMRALLDFERARIDQISAQI 180 Query: 184 DDIAAGSRRLLLILEGLSLLLGCFCAWYLTVGITRPINAALGISRRVAAGDLTTQVSGGD 243 A+ S+ +++LE + LL G A LT ITRP++ AL IS+RVAAGDLT++ Sbjct: 181 QQDASSSQHQIMLLEVVILLCGIAFAVLLTRSITRPVHVALAISQRVAAGDLTSR---AQ 237 Query: 244 GKSADEVGQLLSSLNDMNGSLRNIVSNVRQGTNTIAVASAEISVGNHDLSARTEQQASAL 303 DE+GQL++SL M+ L +VS VR G ++IA+AS EI+ GN DLSARTE+QA AL Sbjct: 238 AMGRDELGQLVASLQGMSERLHGVVSTVRHGADSIAMASGEIANGNLDLSARTEEQAGAL 297 Query: 304 EETASAMEELISTVRQNADNADTASRLAAAASGIAVEGGEVVGRVVTTMSAINGSSRKIV 363 E+TA+++EEL S+VR N+DNA A+ L + A+ +A +GG V+ ++++TM+ IN SSRKIV Sbjct: 298 EQTAASIEELTSSVRTNSDNAHQANELVSTAASVAGQGGAVMDQLISTMATINDSSRKIV 357 Query: 364 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSATAAKEIKLLID 423 DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA AAKEIK LID Sbjct: 358 DIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKQLID 417 Query: 424 DSVKTVGEGGVLVEQAGVTMERVVGSVRKVAEIVGEISLASREQASGIEQVNHAISQMDE 483 DSV V G LVEQAG TM+ VVGSV+ +VGEIS ASR+QA GI QVN A+ QMD Sbjct: 418 DSVGKVSAGSTLVEQAGKTMQEVVGSVQGAHTLVGEISTASRQQAEGIHQVNQAVGQMDG 477 Query: 484 MTQQNAALVEQAAAAASSMQTQAASLAQLVSVFSL 518 +TQQNAALVEQAAAAA S++ QAA L + V+ FSL Sbjct: 478 VTQQNAALVEQAAAAAKSLEDQAAQLKRAVAFFSL 512 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 396 bits (1018), Expect = e-111, Method: Compositional matrix adjust. Identities = 258/515 (50%), Positives = 343/515 (66%), Gaps = 7/515 (1%) Query: 4 MKIGKRLTLGFAAIMLLSIVITAIGIWRLQAVSDVTRAMVEHSARKERLISSWYGSLRAG 63 +KI RL + FA ++ + +IT +G+ L ++S + +VE K L ++ G Sbjct: 6 IKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRDNVN-G 64 Query: 64 ILRXKVRTIAISRSADPTLKTYFIDEAKASTNNSTRLQKEVGELLSSDEEKSLFQNIGER 123 R +R + R+ + T ++D + A++ +T ++ ++LS+ + LFQ+I E Sbjct: 65 AAR-NLRNALLGRNLEEA--TRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQEA 121 Query: 124 RKVYLASHYRILDLKSAGNVEEGNRLLDQEFVPLARSYQDSMLKLLELEQRDIDKTTQQI 183 R Y ++ +L EE LL + +P Y + + + ++ ++++ + Sbjct: 122 RSAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTEG 181 Query: 184 DDIAAGSRRLLLILEGLSLLLGCFCAWYLTVGITRPINAALGISRRVAAGDLTTQVSGGD 243 D + ++L L +++ L AW +T ITRP+N A+ ++ VAAGDLT Q+ Sbjct: 182 RDTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGA-- 239 Query: 244 GKSADEVGQLLSSLNDMNGSLRNIVSNVRQGTNTIAVASAEISVGNHDLSARTEQQASAL 303 S DE G LL++L MN SL IVS VRQG++TI ASAEI+ GN DLS+RTE+QA AL Sbjct: 240 -TSKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGAL 298 Query: 304 EETASAMEELISTVRQNADNADTASRLAAAASGIAVEGGEVVGRVVTTMSAINGSSRKIV 363 EETASAMEEL STV+QNADNA A+ LAA AS +AV+GG VVG+VV TM IN +SRKIV Sbjct: 299 EETASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIV 358 Query: 364 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSATAAKEIKLLID 423 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA+AAKEIK LID Sbjct: 359 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALID 418 Query: 424 DSVKTVGEGGVLVEQAGVTMERVVGSVRKVAEIVGEISLASREQASGIEQVNHAISQMDE 483 DSV V G LVEQAG TM VV SVR+V ++V EIS AS EQ+ GIEQ+NHAI+QMD+ Sbjct: 419 DSVSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMDQ 478 Query: 484 MTQQNAALVEQAAAAASSMQTQAASLAQLVSVFSL 518 +TQQNAALVE+AAAAA S+Q Q+ L+ VSVF L Sbjct: 479 VTQQNAALVEEAAAAAQSLQEQSTRLSATVSVFKL 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.128 0.334 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,122,508 Number of extensions: 36123 Number of successful extensions: 598 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 71 Length of query: 541 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 451 Effective length of database: 1,193,720 Effective search space: 538367720 Effective search space used: 538367720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)