BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0254.p (448 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 400 e-113 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 372 e-104 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 360 e-101 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 281 5e-77 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 272 5e-74 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 400 bits (1029), Expect = e-113, Method: Compositional matrix adjust. Identities = 207/427 (48%), Positives = 298/427 (69%), Gaps = 12/427 (2%) Query: 8 SSLSAATAATATTAAKRKNPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPTQDETT 67 SS +AA A T R+ I ++SIGNALE++D+LIYG FAVVI K FFPT D++ Sbjct: 2 SSNTAAPGADITHPQARR----AIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSV 57 Query: 68 SLLLSVGTFGISFVIRPLGAIVLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQIG 127 SLLL+ TFG+SF +RPLGA+VLG+Y+DRAGRKA+L +SI++M VGTAMIAF P+YA IG Sbjct: 58 SLLLTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIG 117 Query: 128 IASPLIIIVARLLQGFSTGGEFGAATAFMVEHADARRRGFFASWQLSTQGLATVLAAGVS 187 + +P I + +++QGFS GGEFG++TAF+VEHA RRGFF+SWQ+++QG++ +LAA Sbjct: 118 LLAPAGIALGKMIQGFSAGGEFGSSTAFLVEHAP-HRRGFFSSWQVASQGISLLLAALFG 176 Query: 188 ALLSYNLTDVQLNDWGWRVAFCFGLLVGPVGLYIRSQIDETPEFKKIAAEAAVAKAEKSP 247 A+L+ LT QL WGWRV F FGLL+ P G+YIR +DE PEFK+ ++ +P Sbjct: 177 AVLNNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKE------SSEKTNAP 230 Query: 248 LMLVLVRDRVKLLLAIGVVAGATAFNYVHKLYMPTYALKQLHIPATSSFIGALITGLVLM 307 L +++LL+ G V AT Y+ LY+PTYA+KQL +PA SSF + GL++ Sbjct: 231 LRDTFAHQKMRLLIGAGSVIMATVSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMF 289 Query: 308 LTAPVFGTLSDRYGRFRVLTIAMVSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAAC 367 + +P+ G LSD+ GR + + + +YP+F+ L P L+ +Q ++GVL+ Sbjct: 290 IGSPLVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMY 349 Query: 368 LGPIPAMLSDIFPTETRGTGLALSYNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMAT 427 +PA+L+DIFP TRGTG++L+YN +VT+FGGF+ LI+TW+I T +K++ S++V+ Sbjct: 350 FAAMPALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFG 409 Query: 428 ALISLVA 434 A++SL+A Sbjct: 410 AVLSLIA 416 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 372 bits (954), Expect = e-104, Method: Compositional matrix adjust. Identities = 198/441 (44%), Positives = 284/441 (64%), Gaps = 15/441 (3%) Query: 6 ESSSLSAATAATATTAAKRKNPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPTQDE 65 ++S+L A + + +R+ I A IGN LE++D +Y +FAV+I KLFFPT ++ Sbjct: 2 QASTLPADGIVSVSNQQRRR----AIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGND 57 Query: 66 TTSLLLSVGTFGISFVIRPLGAIVLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQ 125 TSLLL+V TFG+ F +RP+G I+LG YADR GRKA+L+V+IL+M VGT MI APTY Sbjct: 58 LTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYES 117 Query: 126 IGIASPLIIIVARLLQGFSTGGEFGAATAFMVEHADARRRGFFASWQLSTQGLATVLAAG 185 IG+ +PLII+VARLLQGFS GGE G+ATAF+ E+A R R +++SW ++ G+A +L A Sbjct: 118 IGLFAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAA 177 Query: 186 VSALLSYNLTDVQLNDWGWRVAFCFGLLVGPVGLYIRSQIDETPEFKKIAAEAAVAKAEK 245 V ++ +L L WGWR+ F G+++GPVG YIR ++ETP F + Sbjct: 178 VGTFVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLE------EKDKTD 231 Query: 246 SPLMLVLVRDRVKLLLAIGVVAGATAFNYVHKLYMPTYALKQLHIPATSSFIGALITGLV 305 SPL V+ + + +V T YV YMPTY++K L +P T+ FI ++ GL Sbjct: 232 SPLKEVIRNFPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLC 291 Query: 306 LMLTAPVFGTLSDRYGRFRVLTIAMVSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIA 365 +M+ AP+ G L+DR GR +L+ + I + +YP+F +N+ P+++TL+ Q + G LIA Sbjct: 292 IMVLAPIVGRLADRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIA 351 Query: 366 ACLGPIPAMLSDIFPTETRGTGLALSYNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVM 425 A GPI A S++FP + TGL+++YN +VT+FGGF+ +TW+IAAT + MAP+FYVM Sbjct: 352 AYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVM 411 Query: 426 ATALISLVAVIVLGRRMGRHP 446 A IS V G R R P Sbjct: 412 IAAAISFV-----GTRFVREP 427 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 360 bits (925), Expect = e-101, Method: Compositional matrix adjust. Identities = 184/411 (44%), Positives = 278/411 (67%), Gaps = 7/411 (1%) Query: 26 NPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPTQDETTSLLLSVGTFGISFVIRPL 85 N +++ AA +GNALE+YD ++YG+F+ +I +LFFP +E TSLL+++ TFGI F +RP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 86 GAIVLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQIGIASPLIIIVARLLQGFST 145 G ++LG YADR GRKA++ V I++M + A+IAF PTYA IGIA+P++I+VAR+LQGF+T Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 146 GGEFGAATAFMVEHADARRRGFFASWQLSTQGLATVLAAGVSALLSYNLTDVQLNDWGWR 205 GGE+ ++TAF+VE A A +RG + SWQL Q LA AG+ AL++ NL+ L+ WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 206 VAFCFGLLVGPVGLYIRSQIDETPEFKKIAAEAAVAKAEKSPLMLV-LVRDRVK-LLLAI 263 + F GLL+GPVGL+IR + ET +F + A K +P+ L+ +V+ ++ +L+++ Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESA-----NKPNAAPIKLLDVVKTNLRGVLVSM 240 Query: 264 GVVAGATAFNYVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGRF 323 G V T YV + MPT+A KQL +P +F+ +I +L P G LSDR GR Sbjct: 241 GQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRR 300 Query: 324 RVLTIAMVSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLSDIFPTET 383 VL + ++ L YPLF+ ++ P++ L+ +Q ++ ++I A GP+P LS+ FPT Sbjct: 301 TVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRV 360 Query: 384 RGTGLALSYNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVA 434 R TGLA++YN + LFGGF+P IVTW+ + + +AP++YV+ A I + A Sbjct: 361 RSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSA 411 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 281 bits (720), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 160/399 (40%), Positives = 241/399 (60%), Gaps = 16/399 (4%) Query: 25 KNPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPTQDETTSLLLSVGTFGISFVIRP 84 K + + AA++GN LE YD +I+G FAV I K FFP+ DE +L+ + TF FV+RP Sbjct: 11 KTQRRAVIAATLGNVLEIYDFIIFGIFAVAISKTFFPSGDEYAALMFTFMTFAAGFVVRP 70 Query: 85 LGAIVLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQIGIASPLIIIVARLLQGFS 144 +GA+VLG YAD+ GRK +L+ ++++M +GT + A P+Y IGIA+ +I++ RLLQGFS Sbjct: 71 IGALVLGQYADKVGRKKALSFTLILMAIGTVIPALCPSYDSIGIAATVILVFGRLLQGFS 130 Query: 145 TGGEFGAATAFMVEHADARRRGFFASWQLSTQGLATVLAAGVSALLSYNL-TDVQLNDWG 203 GGE G+ A +VE+A +R R F S+Q QG +L V +++ + L D+ Sbjct: 131 AGGEIGSNVAMLVENASSRNRALFCSFQQMAQGGGVLLGGIVGYVIAVVYGQERMLQDY- 189 Query: 204 WRVAFCFGLLVGPVGLYIRSQIDETPEFKKIAAEAAVAKAEKSPLMLVLVRDRV-KLLLA 262 WR+AF GL++GPVG YIR + ETPEF++ K E L V+ ++L+ Sbjct: 190 WRLAFFVGLVIGPVGWYIRRVLPETPEFER------SKKKESVDSFLEGVKANFGRILIG 243 Query: 263 IGVVAGATAFNYVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGR 322 I ++ T V Y TYA+++LH+P S+++G + +GL L+ P+ G L+DR G Sbjct: 244 IAIMIFWTVATQVSN-YFTTYAVRELHMPVASTYLGQISSGLTLLAICPLVGMLADRIGP 302 Query: 323 FRVLTIAMVSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGV---LIAACLGPIPAMLSDIF 379 ++ + G+ +YPLF +L PT+ TL+ VQ + V L AAC P ++SDIF Sbjct: 303 YKPMAFGAALAGVLAYPLFKMLVATPTLPTLLLVQIAISVTLGLYAAC---APLVMSDIF 359 Query: 380 PTETRGTGLALSYNFSVTLFGGFSPLIVTWMIAATENKM 418 PT R TG+ SY V +FGG +P+IVT +I + +K+ Sbjct: 360 PTRYRATGIGTSYALGVMIFGGLTPMIVTTLIQISGDKL 398 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 272 bits (695), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 151/425 (35%), Positives = 239/425 (56%), Gaps = 7/425 (1%) Query: 17 TATTAAKRKNPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPTQDETTSLLLSVGTF 76 T TT + + + + GN LE +D +YG++A I K FFP+ +E SL+L+ GTF Sbjct: 2 TTTTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTF 61 Query: 77 GISFVIRPLGAIVLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQIGIASPLIIIV 136 G F++RPLGAI+LGSY DR GR+ L V++ IM +GT +IAF P YA IG+A+P++++ Sbjct: 62 GAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLT 121 Query: 137 ARLLQGFSTGGEFGAATAFMVEHADARRRGFFASWQLSTQGLATVLAAGVSALLSYNLTD 196 RLLQGFS G E G + ++ E A +GF+ SWQ ++Q A + +A + L+S +L+ Sbjct: 122 GRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSP 181 Query: 197 VQLNDWGWRVAFCFGLLVGPVGLYIRSQIDETPEFKKIAAEAAVAKAEKSPLMLVLVRDR 256 VQ++DWGWR+ F G ++ P IR + ET EF + + S + Sbjct: 182 VQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSS------IAQN 235 Query: 257 VKLLLAIGVVAGATAFN-YVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGT 315 K+++A ++ T + Y+ +Y PT+ L + A+ S I GL P+ G Sbjct: 236 WKIVVAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGA 295 Query: 316 LSDRYGRFRVLTIAMVSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAML 375 LSDR GR VL + LT+YP+ L + + L+ + + L A+ G L Sbjct: 296 LSDRVGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVAL 355 Query: 376 SDIFPTETRGTGLALSYNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVAV 435 +++ P R G +L+Y+ + +FGGF+PL+ TW+I + +K AP +++ A+ L+A Sbjct: 356 TEVMPAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLAT 415 Query: 436 IVLGR 440 +VL R Sbjct: 416 LVLYR 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,233,223 Number of extensions: 50208 Number of successful extensions: 357 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 323 Number of HSP's successfully gapped: 17 Length of query: 448 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 360 Effective length of database: 1,203,190 Effective search space: 433148400 Effective search space used: 433148400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)