BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0230.p (232 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309641|ref|YP_003773733.1| heat-shock Hsp70 protein [Herba... 219 1e-58 >gi|300309641|ref|YP_003773733.1| heat-shock Hsp70 protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 219 bits (559), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 122/152 (80%), Positives = 131/152 (86%), Gaps = 2/152 (1%) Query: 56 MSDACGVDFGTSNSTMGWSRQGQPS--LLALEDGKMTLPSVVFFNADENEFSYGRAALST 113 M++ACGVDFGTSNST+GW R P LL LEDGK TLPSVVFFNADEN+FSYGRA L Sbjct: 1 MANACGVDFGTSNSTVGWLRPDAPDSLLLPLEDGKATLPSVVFFNADENQFSYGRAGLGD 60 Query: 114 YLAGYEGRLMRSLKSLLGSGMIDGQTEVGGRALPFRALLSQFIGELKQRGERAAGRRFDS 173 YLAGYEGRLMRSLKSLLGSG IDGQTEVGGRALP+R LLSQFIGELK R +RAAG F S Sbjct: 61 YLAGYEGRLMRSLKSLLGSGAIDGQTEVGGRALPYRGLLSQFIGELKMRAQRAAGTSFSS 120 Query: 174 VVLGRPVHFVDDNAEADQLAQDTLEEIARAVG 205 VVLGRPVHFVDD+ +ADQLAQDTLE+IAR VG Sbjct: 121 VVLGRPVHFVDDDDQADQLAQDTLEQIARDVG 152 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 672,487 Number of extensions: 26669 Number of successful extensions: 82 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 81 Number of HSP's successfully gapped: 1 Length of query: 232 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 150 Effective length of database: 1,231,600 Effective search space: 184740000 Effective search space used: 184740000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)