BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0228.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 87 2e-18 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 67 2e-12 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 62 4e-11 gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 60 2e-10 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 66/203 (32%), Positives = 100/203 (49%), Gaps = 13/203 (6%) Query: 36 QITTAVGETSMLTMADGSRIQLNTDSELRIESRLFSRRMTLLRGE----AGFDVAHTYRS 91 +I TA+GE + + DGSR+ LNTD+ + ++ R + L RGE D R Sbjct: 114 RIATAIGERREVQLDDGSRLWLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPDRQQPPRP 173 Query: 92 FVVEANHTTVTDIGTVFDVRNEEDGARVSVQQGAVEVR-SDSGQSQILAANQALRST-AG 149 F+V ++ +GT F VR + V+V QGAVE+ + S Q +L + R T +G Sbjct: 174 FLVATRQGSLRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQTVLPSGMQTRFTRSG 233 Query: 150 KLERLADPEPGQ--SGAWQRGKLYFNGTPLREAAADMQRYLAQPIVPADARTGDLRLSGE 207 A P P AW RG L PL + ++ RY + ++ AD L +SG Sbjct: 234 S----AAPRPCDDTQAAWSRGLLIAERMPLAQFIGELGRYRSG-LLQADPAIAGLEVSGV 288 Query: 208 YDIKDIDDLLRVLPTILPVTVKR 230 + + D D +LR+L LPV V++ Sbjct: 289 FPLADTDRVLRLLAQTLPVRVQQ 311 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 68/229 (29%), Positives = 101/229 (44%), Gaps = 26/229 (11%) Query: 31 AYDTRQITTAVGETSMLTMADGSRIQLNTDS--ELRIESRLFSRRMTLLR-GEAGFDVAH 87 A D T G+ L + DG+ + LNT S +LR + +RR+ LLR GE D Sbjct: 123 ALDGASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDD---TRRLLLLRQGEIFIDTGA 179 Query: 88 -----TYRSFVVEANHTTVTDIGTVFDVRNEEDGARVSVQQGAVEVRSDSGQSQILAANQ 142 +R F V+ H + +GT FDVR DG +++V +G V V +G ++A Sbjct: 180 DAQWPHHRPFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHV-AGVDPLVAQPG 238 Query: 143 ALRSTA--GKLERLA------DPEPGQSGAWQRGKLYFNGTPLREAAADMQRYLAQPIVP 194 + G+L R A DP AW L L + A + RY + + Sbjct: 239 EIVDIHPDGRLVRRAARASAMDPT-----AWLEQSLVVRQMRLADVVAQLARYRSG-WLQ 292 Query: 195 ADARTGDLRLSGEYDIKDIDDLLRVLPTILPVTVKRGASGVVISAIAGN 243 D D ++SG + ++D D L L LP+T++R V A AG Sbjct: 293 CDPAVADWQVSGVFQLQDTDVALEALAQALPLTIERRTRFWVRVAAAGK 341 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 62.4 bits (150), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 62/217 (28%), Positives = 100/217 (46%), Gaps = 10/217 (4%) Query: 16 AILLLSTGAAWLI---DPAYDTR-QITTAVGETSMLTMADGSRIQLNTDSELRIESRLFS 71 A+L+ G AW + +P R + T +G+ L + DGS++ LNT S + + Sbjct: 98 ALLVFGGGVAWGLTRSEPWQQWRADLRTDIGQQRRLVLEDGSQLVLNTHSAVNLAFSAEE 157 Query: 72 RRMTLLRGEAGFDV--AHTYRSFVVEANHTTVTDIGTVFDVRNEEDGARVSVQQGAVEV- 128 RR+ L+ GE A R F+VE +GT + VR VSV +GAV + Sbjct: 158 RRLRLISGELLVTTGKAGGGRPFLVETAQGLAYALGTRYAVRQRAGRTEVSVWEGAVRLV 217 Query: 129 -RSDSGQSQILAANQALRSTAGKLERLADPEPGQSGAWQRGKLYFNGTPLREAAADMQRY 187 R + Q+++L A Q +A + + + + AW G + G L A++ RY Sbjct: 218 PRQAAQQARVLQAGQQAAFSAQGVGDVTTADETRM-AWTDGVIVARGMRLDAFLAELGRY 276 Query: 188 LAQPIVPADARTGDLRLSGEYDIKDIDDLLRVLPTIL 224 + A A +G +R+SG Y + D D ++ L L Sbjct: 277 TTDRLSCAPAVSG-VRISGSYPVADSDAVIATLAAAL 312 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 58/206 (28%), Positives = 93/206 (45%), Gaps = 13/206 (6%) Query: 26 WLIDPAYDTRQIT----TAVGETSMLTMADGSRIQLNTDSELRIESRLFSRRMTLLRGEA 81 WL AY R + TA G T+ADGSR+ L+ S R + L++GE Sbjct: 114 WLGLQAYPARYLLADMRTATGHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGEL 173 Query: 82 GFDVAH-TYRSFVVEANHTTVTDIGTVFDVRNEEDGARVSVQQGAVEVRSDSGQSQ---I 137 +VA R FV++ ++ +GT F VR + G VS+ + V ++ + +Q + Sbjct: 174 LVEVARDPARPFVIQTADGSIRALGTRFLVRRDRAGTLVSMLESKVSIQPGADTTQAPLV 233 Query: 138 LAANQALR---STAGKLERLADPEPGQSGAWQRGKLYFNGTPLREAAADMQRYLAQPIVP 194 L A Q +R AG LE + AW+R +L N PL E ++ R+ I+ Sbjct: 234 LEAGQRVRLHGGAAGPLEHID--AAALERAWRRHQLVSNARPLTEVLDELNRHRPGRILY 291 Query: 195 ADARTGDLRLSGEYDIKDIDDLLRVL 220 A+ L ++ + D L++L Sbjct: 292 DQAQLAGLHMTVVLPLDDTQRALQLL 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 743,386 Number of extensions: 31246 Number of successful extensions: 138 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 130 Number of HSP's successfully gapped: 5 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)