BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0225.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 62 4e-11 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 59 5e-10 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 58 9e-10 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 57 2e-09 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 62.4 bits (150), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 40/109 (36%), Positives = 61/109 (55%), Gaps = 3/109 (2%) Query: 153 EKAQKIAIASAVVAMLLFSLVVVATL---HRSLKPLRKASAAAGQIQVDNLSARLSLDDV 209 E + +A++V ++L L V+++ L PLR+ S A LSAR+ ++ + Sbjct: 161 EMRRTFLLATSVFSLLGIVLAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAM 220 Query: 210 PSELAPLFNAFNLALQRLEDGYRVQREFLATAAHELKTPLALMRGQVEL 258 P EL+ L AFN AL R+ED Y F A AHEL+TPLA + G+ ++ Sbjct: 221 PQELSVLALAFNGALDRIEDAYTQLEAFNADVAHELRTPLANLIGETQV 269 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 58.9 bits (141), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 48/149 (32%), Positives = 74/149 (49%), Gaps = 15/149 (10%) Query: 139 RSERMHRIMLGSDSEKAQKIA---IASAVVAMLLFSLVVVA----TLHRSLKPLRKASAA 191 R R+ + +G EK ++A I ++ + V +A L R L PL + Sbjct: 48 REPRLATVQVGETLEKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQR 107 Query: 192 AGQIQVDNLSARLSLDDVPSELAPLFNAFNLALQRLEDGYRVQREFLATAAHELKTPLAL 251 + D+LS + VP E++PL + N L RL +Q+ F+A AAH++KTPLA Sbjct: 108 IRARRPDDLSP-IDSGQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAG 166 Query: 252 MRGQVEL----DDRGDRGALLRDLDHMAR 276 MR Q EL DD+ D + R L+ +A+ Sbjct: 167 MRMQSELALRQDDQVD---VRRSLEQLAK 192 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 58.2 bits (139), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 35/86 (40%), Positives = 52/86 (60%), Gaps = 5/86 (5%) Query: 178 LHRSLKPLRKASAAAGQIQVDNLSARLSLDDV--PSELAPLFNAFNLALQRLEDGYRVQR 235 + R L P+RKA+A ++ ++++ L DV P E+ PL +A N L RL+ QR Sbjct: 161 VSRGLAPVRKAAA---EVAARDMNSLQPLADVGMPQEIQPLTHALNDLLARLKHATETQR 217 Query: 236 EFLATAAHELKTPLALMRGQVELDDR 261 F+A AAHEL+TPL +R Q +L +R Sbjct: 218 AFVADAAHELRTPLTALRLQTQLAER 243 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 31/76 (40%), Positives = 46/76 (60%) Query: 183 KPLRKASAAAGQIQVDNLSARLSLDDVPSELAPLFNAFNLALQRLEDGYRVQREFLATAA 242 +PL S A QI D+LSARL + +P+EL + +FN L RLE GY +F A Sbjct: 200 QPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAGYAHLAQFSGDLA 259 Query: 243 HELKTPLALMRGQVEL 258 HE++TP+ ++ GQ ++ Sbjct: 260 HEIRTPINVLMGQSQV 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 831,466 Number of extensions: 29440 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 6 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)