BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0188.p (501 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 107 3e-24 gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 102 6e-23 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 84 3e-17 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 107 bits (267), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 106/388 (27%), Positives = 162/388 (41%), Gaps = 57/388 (14%) Query: 7 GAGPVGLFLACELRLAGVSVLVLEQTDNPQSPLKQLPFGIRGLSIPTVEAFHWRGLLDAV 66 GAGPVGL A +L G++V++++ D + + + F R L I D + Sbjct: 37 GAGPVGLACAIDLARQGIAVVLVDDDDTLSTGSRAICFAKRTLDI-----------FDRI 85 Query: 67 KAPSPVANASASTPHWMQQPRRAGGHFAGMQFYLDQVDRSKWPTHLPGPAXG-DAGIATD 125 + + + G G F DQ LP A I Sbjct: 86 GCGQRMVD-------------KGVGWNRGKVFLRDQAVYEF--DLLPESGHARPAFINLQ 130 Query: 126 MQHIESVLAARALTM-GVEIMRGAGVDGLRQSDEH----VDIHAGGQTFRARWLVGCDGG 180 ++E L +A +EI V + ++H VD G T +AR++V DG Sbjct: 131 QYYVEGYLLEQAQQWPHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLKARYVVAADGS 190 Query: 181 RSTVRKIGGFEFAGTEPEFTGYSVEVALADPQLLPPGRHYTATGMYTFQPP--------- 231 RS +RK+ G E G + +AD ++ P + A + F PP Sbjct: 191 RSPLRKMMGLESQGHT-----FRDRFLIADVKMDAP---FPAERWFWFDPPFHPQQSVLL 242 Query: 232 ----GTVAMVDFDGGAFHRTQPITREYVESVLRRISGVGALNVT-ALRLAATWTDRAFQA 286 V +DF G P+ + E V+ R+ + V+ +L + +T + Sbjct: 243 HRQPDDVWRIDFQLG--WDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRM 300 Query: 287 ATYREGRILLAGDAAHVHSPLGGQGLNLGLGDAMNLGWKLAATIRGDAPDGLLDSYETER 346 +R GR+L GDAAH SP G +G N G+ DA NL WKLA ++G APD LLDSY +ER Sbjct: 301 ERFRHGRVLFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 Query: 347 -HPVGTRILDWSRAQVGLMRPGESSRAL 373 + +L+ +R+ + SRA Sbjct: 361 EYAADENLLNSTRSTDFITPKSAVSRAF 388 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 102 bits (255), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 111/355 (31%), Positives = 157/355 (44%), Gaps = 46/355 (12%) Query: 7 GAGPVGLFLACELRLAGVSVLVLEQT----DNPQSPLKQLPFGIRGLSIPTVEAFHWRGL 62 GAGPVG+ L L G+S L++E+ D P++ G+ + F G+ Sbjct: 23 GAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAV---------GIDDEALRTFQSAGM 73 Query: 63 LDAVKAPSPVANASASTPHWMQQPRRAGGHFAGMQFYLDQVDRSKWPTHLPGPAXGDAGI 122 DA+ V + + P M R FA +Q + + +L G+A + Sbjct: 74 ADAI-----VRDVIQNVPMRMYTADRRC--FAEIQPTTREF--GWYRRNLFSQPLGEATL 124 Query: 123 ATDMQHIESVLAARALTMGVEIMRGAGVDGLRQSDEHVDIHAGGQTFRARWLVGCDGGRS 182 ++ E V +L GVE++ + Q+ +D H T RA+++VGCDGGRS Sbjct: 125 RLGLRRFEHV----SLRSGVELLDLVQDEAGVQA-SIIDEHGAQATLRAQYVVGCDGGRS 179 Query: 183 TVR-KIGGFEFAGTEPEFTGYSVEVALADPQLLP-------PGRHYTATGM-YTFQPPGT 233 TVR KI + G +E DP P P R Y + Y + Sbjct: 180 TVREKILKLPYEGKTHPAKWVVIECD-GDPLDAPYTALHCEPQRPYVCLALPYGVRRWEF 238 Query: 234 VAMVDFDGGAFHRTQPITREYVESVLRR-ISGVGALNVTALRLAATWTDRAFQAATYREG 292 + DG Q + E V +LR + V ALN+ R+ +T + A + G Sbjct: 239 MLFPGEDG-----EQMLAPEKVRELLREHVRDVDALNIIRARV---YTHNSRVAGSLVCG 290 Query: 293 RILLAGDAAHVHSPLGGQGLNLGLGDAMNLGWKLAATIRGDAPDGLLDSYETERH 347 RI LAGDAAH+ P GQGLN GL DA NL WKLA ++G LL SY ERH Sbjct: 291 RICLAGDAAHITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEERH 345 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 83.6 bits (205), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 76/255 (29%), Positives = 104/255 (40%), Gaps = 29/255 (11%) Query: 152 GLRQSDEH--VDIHAGGQTFR--ARWLVGCDGGRSTVRKIGGFEFAGTEPEFTGYSVEVA 207 L Q D H +D+ FR A W++ CDG S RK+ G EF+G +F + Sbjct: 153 SLHQDDTHATLDVETPDGIFRMEADWVIACDGANSDTRKMVGAEFSG---QF--FQDRFL 207 Query: 208 LADPQLLPPGRHYTATGMYTFQPP-------------GTVAMVDFDGGAFHRTQPITREY 254 +AD + + A + F PP V +DF G P + Sbjct: 208 IADVIM---KADFPAERWFWFDPPFHRGQSVLLHKQCDNVWRIDFQLG--WEADPEEEKK 262 Query: 255 VESVLRRISGVGALNVT-ALRLAATWTDRAFQAATYREGRILLAGDAAHVHSPLGGQGLN 313 E V+ RI + V L + + + R R+L AGDAAH SP G +G N Sbjct: 263 PEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRYQRVLFAGDAAHQVSPFGARGAN 322 Query: 314 LGLGDAMNLGWKLAATIRGDAPDGLLDSYETERHPVGTRILDWSRAQVGLMRP-GESSRA 372 G+ D NL WKL I G AP +DSY ER L S + P +S+A Sbjct: 323 TGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAADDNLRQSTRSTDFITPKSRASKA 382 Query: 373 LAAVIRDLIGTRDGA 387 L + L T + A Sbjct: 383 LRDAVLGLAETEEFA 397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,766,167 Number of extensions: 81755 Number of successful extensions: 262 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 3 Length of query: 501 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 412 Effective length of database: 1,198,455 Effective search space: 493763460 Effective search space used: 493763460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)