BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0184.p (257 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 310 6e-86 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 144 1e-35 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 131 5e-32 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 122 2e-29 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 122 3e-29 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 310 bits (795), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 150/246 (60%), Positives = 188/246 (76%) Query: 12 PLILITGGSRGVGAATARLAAVQGYDVAISFVSNESAALAVAADVEALGRRALAVRADSA 71 +IL+TGGSRG+GAATA LA +GY VA+++V+N +AA AV A ++A G RA+AV D A Sbjct: 6 KVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGGDVA 65 Query: 72 DPEQVAQLFTAIDRSFGRIDVLVNNAAMLSPQSRLEDLGFARMQRIFAVNAIGPILCAQQ 131 E V QLF +D GR+ LVNNA +L QSRLED+ R+ R+ N G +LCA++ Sbjct: 66 REEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLCARE 125 Query: 132 AVKRMSTRHNGRGGVVVNISSASARLGSPNEYVDYAASKGALETFTTGFAKEVAREGIRV 191 AVKRMSTRH G+GG +VN+SS +A+LG P EYVDYAASKGA++ FT G AKEVA EGIRV Sbjct: 126 AVKRMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRV 185 Query: 192 NCIRPGHIYTEMHASGGEPGRVDRVKDSIPMGRGGQPEEVARAILWLASAEASFITGTFL 251 N +RPG IYT++HASGGE GRVDR+KD++PM RGG EVA AILWL S EAS+ TGTF+ Sbjct: 186 NAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATGTFI 245 Query: 252 DVTGGK 257 DV+GG+ Sbjct: 246 DVSGGR 251 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 144 bits (362), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 86/243 (35%), Positives = 132/243 (54%), Gaps = 6/243 (2%) Query: 15 LITGGSRGVGAATARLAAVQGYDVAISFVSNESAALAVAADVEALGRRALAVRADSADPE 74 LITGG G+G A A A +G +AI ++ E A DV+AL L + D PE Sbjct: 129 LITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVGSPE 188 Query: 75 QVAQLFTAIDRSFGRIDVLVNNAAMLSPQSRLEDLGFARMQRIFAVNAIGPILCAQQAVK 134 A+ ++GR+DVLVNNAA P +E++ A+++ F N ++A+ Sbjct: 189 FCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYMVKEAMP 248 Query: 135 RMSTRHNGRGGVVVNISSASARLGSPNEYVDYAASKGALETFTTGFAKEVAREGIRVNCI 194 ++ RG ++N +S +A GS VDYAA+KGA+ FT ++++A GI VN + Sbjct: 249 FLT-----RGARIINTTSVTAYRGS-KHLVDYAATKGAIVAFTRSLSQQLASAGIHVNAV 302 Query: 195 RPGHIYTEMHASGGEPGRVDRVKDSIPMGRGGQPEEVARAILWLASAEASFITGTFLDVT 254 PG I+T + + V ++P+GR GQP+EVA A ++LA+ AS++TG L Sbjct: 303 APGPIWTPLIPASFSAEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGASYMTGQVLHPN 362 Query: 255 GGK 257 GG+ Sbjct: 363 GGE 365 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 131 bits (330), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 92/247 (37%), Positives = 138/247 (55%), Gaps = 18/247 (7%) Query: 15 LITGGSRGVGAATARLAAVQGYDVAISFVSNESAALAVAADVEALGRRALAVRADSADPE 74 + GGSRG+GAA + A +G VA ++VS+ A A+ + V+A G RALA++ADSAD Sbjct: 21 FVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQADSADAA 80 Query: 75 QVAQLFTAIDRSFGRIDVLVNNAAMLS----PQSRLEDLGFARMQRIFAVNAIGPILCAQ 130 + Q +FGR+D+LVNNA +L+ Q +LEDL R AVN + Q Sbjct: 81 ALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDL-----DRTLAVNVRSVFVATQ 135 Query: 131 QAVKRMSTRHNGRGGVVVNISSASA-RLGSPNEYVDYAASKGALETFTTGFAKEVAREGI 189 +A R+ G GG V++I S +A R+ V YA SK AL T G A+++ GI Sbjct: 136 EA-----ARYMGEGGRVISIGSTNADRIPFAGGAV-YAMSKSALVGLTKGLARDLGPRGI 189 Query: 190 RVNCIRPGHIYTEMHASGGEPGRVDRVKDSIPMGRGGQPEEVARAILWLASAEASFITGT 249 VN ++PG + T+M+ + G+ + + + R G+ EE+A + +LA E+ +ITG Sbjct: 190 TVNNVQPGPVDTDMNPASGD--FAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITGA 247 Query: 250 FLDVTGG 256 L + GG Sbjct: 248 NLMIDGG 254 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 122 bits (307), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 87/247 (35%), Positives = 135/247 (54%), Gaps = 13/247 (5%) Query: 13 LILITGGSRGVGAATARLAAVQGYDVAISFVSNESAALAVAADVEALGRRALAVRADSAD 72 ++++TGGS G+G ATA+L A +G V +VS A A V +G A+ V D+A Sbjct: 11 VVVVTGGSEGIGLATAKLFAAEGARV---YVSGRRADKLEQA-VREIGNGAVGVPGDAAK 66 Query: 73 PEQVAQLFTAIDRSFGRIDVLVNNAAMLSPQSR-LEDLGFARMQRIFAVNAIGPILCAQQ 131 + + +L+ + GR+DVL NA S +++ L +G +F +N G + Q+ Sbjct: 67 LDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFSVQK 126 Query: 132 AVKRMSTRHNGRGGVVVNISSASARLGSPNEYVDYAASKGALETFTTGFAKEVAREGIRV 191 A+ MS +GG +V S + G P + + Y ASK A+ +F + ++ GIRV Sbjct: 127 ALPLMS-----QGGAIVLNGSVAGVKGFPGQSL-YNASKAAVRSFARSWTADLKERGIRV 180 Query: 192 NCIRPGHIYTEMHASGGE--PGRVDRVKDSIPMGRGGQPEEVARAILWLASAEASFITGT 249 N + PG T + E PG D +K +P+GR G+P+E+ RA+L+LASAE+S+I G Sbjct: 181 NVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAGV 240 Query: 250 FLDVTGG 256 L V GG Sbjct: 241 ELFVDGG 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 122 bits (307), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 88/254 (34%), Positives = 134/254 (52%), Gaps = 8/254 (3%) Query: 4 LSRSQSQAPLILITGGSRGVGAATARLAAVQGYDVAISFVSNESAALAVAADVEALGRRA 63 ++R+ S A + L+TGG+ G+GA+ ++ A +G+ V ++ + N AA A + A G RA Sbjct: 1 MNRNDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADI-NLDAASKTADALVAAGHRA 59 Query: 64 LAVRADSADPEQVAQLFTAIDRSFGRIDVLVNNAAMLSPQSRLEDLGFARMQRIFAVNAI 123 ++ D PE +A F I++ +GR DVLVNNA + S L D + VN Sbjct: 60 APLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFL-DYPLENWLQTMNVNVT 118 Query: 124 GPILCAQQAVKRMSTRHNGRGGVVVNISSASA-RLGSPNEYVDYAASKGALETFTTGFAK 182 G +L Q A + M + GR +VNISS S R G+ Y SK A+ T A Sbjct: 119 GVLLAGQHAARLMVKQQWGR---IVNISSISGIRAGAGR--TAYGTSKAAVIGLTRQMAI 173 Query: 183 EVAREGIRVNCIRPGHIYTEMHASGGEPGRVDRVKDSIPMGRGGQPEEVARAILWLASAE 242 E+A+ GI VN + PG + T M + + +PM R G +E+A A+ +LA+ E Sbjct: 174 ELAQYGITVNSVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEE 233 Query: 243 ASFITGTFLDVTGG 256 +++ITG + V GG Sbjct: 234 SAYITGHVIPVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 760,890 Number of extensions: 29141 Number of successful extensions: 304 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 45 Length of query: 257 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 174 Effective length of database: 1,226,865 Effective search space: 213474510 Effective search space used: 213474510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)