BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0164.p (334 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subun... 653 0.0 gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate ... 114 2e-26 gi|300309646|ref|YP_003773738.1| transketolase C-terminal subuni... 96 4e-21 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 60 2e-10 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 59 4e-10 >gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 334 Score = 653 bits (1684), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/334 (93%), Positives = 327/334 (97%) Query: 1 MNTVAEKKPRLTTSAMIASIAAEGQRVQAAPFGKALVEYAADRPEIVGMTADLAKYTDLQ 60 MNTVA+KKPRLTTSAMIASIAAEGQRVQAAPFGKALV+YAADRPEIVG+TADLAKYTDL Sbjct: 1 MNTVADKKPRLTTSAMIASIAAEGQRVQAAPFGKALVDYAADRPEIVGLTADLAKYTDLH 60 Query: 61 LFQKAYPERFFQMGMAEQLLMAAAGGMAKEGLVPFATTYAVFATRRAYDFIHQVIAEENL 120 LFQKAYPERFFQMGMAEQLLMAAAGGMAKEGLVPFATTYAVFATRRAYDFIHQVIAEENL Sbjct: 61 LFQKAYPERFFQMGMAEQLLMAAAGGMAKEGLVPFATTYAVFATRRAYDFIHQVIAEENL 120 Query: 121 NVKICCALPGLTTGYGPSHQATEDLAMMRAIPGLTIVDPCDALDLEQAVPQIAEHKGPVY 180 NVKICCALPGLTTGYGPSHQATEDLAMMRAIPG+TIVDPCDALDLEQAVPQIA H+GPVY Sbjct: 121 NVKICCALPGLTTGYGPSHQATEDLAMMRAIPGMTIVDPCDALDLEQAVPQIAAHQGPVY 180 Query: 181 MRLARGNVPLVLDEYDYKFELGKAKELRDGNDVLIISSGFMTMRALEVAKALEADNVSVG 240 MRLARGNVPLVLDEYDY+FELG+AKE+RDGNDVLIISSGFMTMRALEVAKALEAD VSV Sbjct: 181 MRLARGNVPLVLDEYDYRFELGRAKEIRDGNDVLIISSGFMTMRALEVAKALEADKVSVA 240 Query: 241 VLHCPTIKPFDEATVLEQVKKKTRLVVVAENHSVIGGLGEIVASTLMRNRVQPAGFQMAG 300 VLHCPTIKPFDEATV+ QVKKK RLVVVAENHSV+GGLGEIVA+TLMR+RVQPAGFQ+AG Sbjct: 241 VLHCPTIKPFDEATVVAQVKKKNRLVVVAENHSVVGGLGEIVATTLMRHRVQPAGFQLAG 300 Query: 301 LPDAFLDAGALPTLHDRYGISCEVLSRRIRDWLK 334 LPDAFLDAGALPTLHDRYGISCEVL RRI++WLK Sbjct: 301 LPDAFLDAGALPTLHDRYGISCEVLGRRIKEWLK 334 >gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate synthase [Herbaspirillum seropedicae SmR1] Length = 621 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 85/310 (27%), Positives = 146/310 (47%), Gaps = 9/310 (2%) Query: 21 AAEGQRVQAAPFGKALVEYAADRPEIVGMTADLAKYTDLQLFQKAYPERFFQMGMAEQLL 80 +A G+ FG L + AA ++VG+T + + + + F+K +P R++ +G+AEQ Sbjct: 304 SAGGKLTYTKVFGDWLCDMAAADDKLVGITPAMREGSGMVDFEKKFPNRYYDVGIAEQHA 363 Query: 81 MAAAGGMAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCALPGLTTGYGPSHQ 140 + A GMA EG+ P Y+ F +RAYD + +A +NL+V GL G +H Sbjct: 364 VTFAAGMACEGMKPVVAIYSTF-LQRAYDQLIHDVALQNLDVTFALDRAGLVGADGATHA 422 Query: 141 ATEDLAMMRAIPGLTIVDPCDALDLEQAVPQIAEHKGPVYMRLARG-NVPLVLDEYDYKF 199 DLA +R IP + ++ D + + + ++ GP +R RG + +D+ Sbjct: 423 GNYDLAFLRCIPNMVVMAASDENECRKMLSTAYQYPGPASVRYPRGAGIGAAVDQTLETL 482 Query: 200 ELGKAKELRDGNDVLIISSGFMTMRALEVAKALEADNVSVGVLHCPTIKPFDEATVLEQV 259 LG+ + R G +V I++ G + +L L A V + IKP D +++Q+ Sbjct: 483 PLGRGEIRRQGKNVAILAFGSLLAPSLGAGDKLNAT-----VANMRFIKPLD-VELVKQL 536 Query: 260 KKKTRLVVVAENHSVIGGLGEIVASTLMRNRVQPAGFQMAGLPDAFLDAGALPTLHDRYG 319 +V E ++GG G V+ L + + GLPD F+D G L + G Sbjct: 537 AASHDALVTVEEGCIMGGAGAAVSEALAAAGINKPILHL-GLPDRFIDHGDAAQLLAQCG 595 Query: 320 ISCEVLSRRI 329 + ++ I Sbjct: 596 LDANGIADSI 605 >gi|300309646|ref|YP_003773738.1| transketolase C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 321 Score = 95.9 bits (237), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 69/274 (25%), Positives = 128/274 (46%), Gaps = 10/274 (3%) Query: 66 YPERFFQMGMAEQLLMAAAGGMAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKIC 125 +P+RF +G+AEQ L+ + G+A EG FA A F T R Y+ + +A + + Sbjct: 45 FPDRFVNVGIAEQNLINVSAGLALEGYKVFAYAIAPFITMRCYEQVRVSLALLSEVRPMN 104 Query: 126 CALPGLTTGY-----GPSHQATEDLAMMRAIPGLTIVDPCDALDLEQAVPQIAEHKGPVY 180 L G+ GY GP+HQ ED+ +MRA+P ++ D + + GP Y Sbjct: 105 VNLIGVGAGYSYVVSGPTHQCYEDITLMRALPNFQVLSAADHVTAAAFFDTCSNSMGPKY 164 Query: 181 MRLARGNVPLVLDEYDYKFELGKAKEL-RDGNDVLIISSGFMTMRALEVAKALEADNVSV 239 +R +P++ D +L + + R G + ++++G+M AL+ A+ L A+ + V Sbjct: 165 LRFDAQVLPVIYG--DGAPDLAQGFHIHRRGGKIGLVATGYMLHTALQAAEVLAAEGIEV 222 Query: 240 GVLHCPTIKPFDEATVLEQVKKKTRLVVVAENHSVIGGLGEIVASTLMRNRVQPAGFQMA 299 GV+ + F + E ++ + + E GG+ ++ + R +Q + Sbjct: 223 GVIDLFDLARFSADALQETLQSYAGIATLEEGFRGRGGMDSMLFEFIARRGLQTRMLNI- 281 Query: 300 GLPDAF-LDAGALPTLHDRYGISCEVLSRRIRDW 332 G+ + + G LH+ GI + + IR++ Sbjct: 282 GVDGVYRFELGTRTELHEEVGIGPQAVLTSIREF 315 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 60.5 bits (145), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 68/272 (25%), Positives = 116/272 (42%), Gaps = 29/272 (10%) Query: 34 KALVEYAADRPEIVGMTADLAKYTDLQLFQKAYPER------FFQMGMAEQLLMAAAGGM 87 A+ YA PE +G +ADL ++L ++++ R G+ E + A G+ Sbjct: 365 NAIQAYAQALPEFLGGSADLTG-SNLTNWKESVAVRADQRGNHINYGVREFGMSAIMNGI 423 Query: 88 A-KEGLVPFATTYAVFATRRAYDFIHQVIAEENL-NVKICCALPGLTTGYG---PSHQAT 142 A G +PF T+ F+ D+ + L ++ + G G P+HQ+ Sbjct: 424 ALHGGYLPFGATFLTFS-----DYSRNALRMAALMKIRALFVFTHDSIGLGEDGPTHQSV 478 Query: 143 EDLAMMRAIPGLTIVDPCDALDLEQAVPQ-IAEHKGPVYMRLARGNVPLVLDEYDYKFEL 201 E ++ +R IP L PCD + A + I H GP + +R N+P ++ Sbjct: 479 EHVSSLRLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKDI 538 Query: 202 GKAKE-LRDGND--VLIISSGFMTMRALEVAKALEADNVSVGVLHCPTIKPFDEATVLEQ 258 + LRD + V+++++G A + A AL A+ ++V V+ P+ FD + Sbjct: 539 ARGGYVLRDAPNAKVILMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQDAAYK 598 Query: 259 VKKKTRLV--------VVAENHSVIGGLGEIV 282 TR V V A H +G G +V Sbjct: 599 ASVLTRGVPRVAIEAGVTAFWHKYVGLEGAVV 630 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 52/191 (27%), Positives = 88/191 (46%), Gaps = 10/191 (5%) Query: 70 FFQMGMAEQLLMAAAGGMAKEG-LVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCAL 128 + G+ E L+ A A GMA G ++ + TY F+ ++ A L VK + Sbjct: 400 YVHCGVREHLMGAMANGMAAHGGVIALSVTYLAFSDYERPAM--RMSALMGLPVKYVFSH 457 Query: 129 PGLTTG-YGPSHQATEDLAMMRAIPGLTIVDPCDALDLEQAVPQIAEHK-GPVYMRLARG 186 + G GP+HQ E LA +RA+P + ++ P DA + + +H+ GPV M AR Sbjct: 458 DSIGIGKNGPTHQPVEILASLRAMPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQ 517 Query: 187 NVPLVLDEYDYKFELGKA----KELRDG-NDVLIISSGFMTMRALEVAKALEADNVSVGV 241 +P V +Y+ + + E + G V ++++G A LEA ++ V Sbjct: 518 ALPPVRRQYEAENRCARGGYVLHEAQCGARQVTLLATGSEVALACAARAQLEAAGIATAV 577 Query: 242 LHCPTIKPFDE 252 + P + FD+ Sbjct: 578 VSLPCWELFDQ 588 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,024,711 Number of extensions: 42564 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 5 Length of query: 334 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 248 Effective length of database: 1,212,660 Effective search space: 300739680 Effective search space used: 300739680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)