BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0163.p (281 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 505 e-144 gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 148 5e-37 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 107 1e-24 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 107 1e-24 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 505 bits (1300), Expect = e-144, Method: Compositional matrix adjust. Identities = 241/281 (85%), Positives = 262/281 (93%) Query: 1 MSENLESLRASAYRIRRFAVRMGEVQGQGYIGQALGWADVLAVAYKRAMQIRPMEPEWEG 60 MS+NL +LRASAYRIRR AVRMGEVQGQGYIGQALGWADVLAVAYK AM +RP +P+W G Sbjct: 1 MSDNLANLRASAYRIRRNAVRMGEVQGQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSG 60 Query: 61 RDRFLLSHGHYAIAAYAALIEAGILPEEELETYGSDDSRLPMSGMAAYTPGMEISGGSLG 120 RDRFLLSHGHYAIA YAALIEAGI+P+EEL++YGSDDSRLPMSGMA YTPGMEISGGSLG Sbjct: 61 RDRFLLSHGHYAIALYAALIEAGIVPDEELDSYGSDDSRLPMSGMATYTPGMEISGGSLG 120 Query: 121 QGLAIGVGMALALRHKGSASFVYNSMSDGELDEGATWEAAMGAAHHNLGNLICLVDINNQ 180 QGLAIGVGMAL LR+ + +FVYNSMSDGELDEG+TWEAAMGAAHH LGNLICLVDINNQ Sbjct: 121 QGLAIGVGMALGLRYNNNPAFVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDINNQ 180 Query: 181 QADGPSGKIMGFEPLADKWASFGWHVQRVDGNDLPAVIAAFDQARQLPESKPRVILFDTL 240 QADGPSGKIMGFEPLADKWA+FGW+VQRVDGNDL AV+ AFD AR L ++KPRVILFDTL Sbjct: 181 QADGPSGKIMGFEPLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDTL 240 Query: 241 MGKGVPFLEQREKNHFIRVDPPEWQQALNILDQSYKEGSPA 281 MGKGVPFLEQREKNHFIRVDPPEWQQAL+ILDQSY++GS A Sbjct: 241 MGKGVPFLEQREKNHFIRVDPPEWQQALSILDQSYQQGSQA 281 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 148 bits (373), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 96/263 (36%), Positives = 132/263 (50%), Gaps = 4/263 (1%) Query: 1 MSENLESLRASAYRIRRFAVRMGEVQGQGYIGQALGWADV-LAVAYKRAMQIRPMEPEWE 59 M + LRA A+ + R + + + + +L D+ +A+ Y ++ P +P Sbjct: 1 MKNDFVDLRAKAHWVWRETLAIHRRAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDA 60 Query: 60 GRDRFLLSHGHYAIAAYAALIEAGILPEEELETYGSDDSRLPMSGMA-AYTPGMEISGGS 118 GRDR ++S GH +I Y L + G P EEL+ S L G+ PG E GS Sbjct: 61 GRDRCVISKGHGSICMYPILADLGYFPMEELQHVCEAGSFL--GGIPDPVIPGYETVNGS 118 Query: 119 LGQGLAIGVGMALALRHKGSASFVYNSMSDGELDEGATWEAAMGAAHHNLGNLICLVDIN 178 LG GL + GMAL L+ GS V+ DGEL EGA WEA M A+ H L NL +VD N Sbjct: 119 LGHGLGVATGMALGLKRSGSDRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDN 178 Query: 179 NQQADGPSGKIMGFEPLADKWASFGWHVQRVDGNDLPAVIAAFDQARQLPESKPRVILFD 238 G + I+ LA + ++FGW VDG+D+ AV AA Q + KP+ I+ Sbjct: 179 RISMLGYTEDIVSHGELATRLSAFGWDCMEVDGHDVLAVQAALLQQKAHAAGKPKAIIAR 238 Query: 239 TLMGKGVPFLEQREKNHFIRVDP 261 TL G GVP LE +H I P Sbjct: 239 TLKGHGVPGLENAPLSHIINPKP 261 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 85/258 (32%), Positives = 125/258 (48%), Gaps = 19/258 (7%) Query: 12 AYRIRRFAVRMGEVQGQGYIGQALGWADVLAVAYKRAMQIRPMEPEWEGRDRFLLSHGHY 71 A IR A+ + G+ G +G A++ + + + P P W RDRF+LS+GH Sbjct: 11 ANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPANPHWANRDRFVLSNGHG 70 Query: 72 AIAAYAALIEAGI-LPEEELETYGSDDSRLPMSGMAAYTPGMEISGGSLGQGLAIGVGMA 130 ++ YA L G L E+++++ S+ TPG+E + G LGQG+ VGMA Sbjct: 71 SMLQYALLHLTGYDLSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLGQGITNAVGMA 130 Query: 131 LA-------LRHKGSA---SFVYNSMSDGELDEGATWEAAMGAAHHNLGNLICLVDINNQ 180 LA G A + Y + DG L EG + EA A NL LI L D N Sbjct: 131 LAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSKLIVLYDDNGI 190 Query: 181 QADGPSGKIMGF--EPLADKWASFGWHV-QRVDGNDLPAVIAAFDQARQLPESKPRVILF 237 D G + G+ + ++ ++GW+V VDG+ + AV AA QA+ KP +I Sbjct: 191 SID---GHVEGWFKDDTPKRFEAYGWNVIPAVDGHSVEAVSAAIHQAKL--ADKPTLICC 245 Query: 238 DTLMGKGVPFLEQREKNH 255 T++GKG P L +K H Sbjct: 246 RTVIGKGSPNLAGTDKVH 263 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 86/280 (30%), Positives = 136/280 (48%), Gaps = 18/280 (6%) Query: 4 NLESLRASAYRIRRFAVRMGEVQGQGYIGQALGWADVLAVAYKRAMQIRPMEPEWEGRDR 63 N L+ A+ IR ++ +G+ G LG A++ Y R ++ P P W RDR Sbjct: 2 NEHPLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRDR 61 Query: 64 FLLSHGHYAIAAYAALIEAGI--LPEEELETYGSDDSRLPMSGMAAYTP--GMEISGGSL 119 +LS+GH ++ Y L G + E++ ++ S +G Y P G+E++ G L Sbjct: 62 VVLSNGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHC--AGHPEYAPELGIEVTTGPL 119 Query: 120 GQGLAIGVGMALALRHKGSASF--------VYNSMSDGELDEGATWEAAMGAAHHNLGNL 171 GQG+A GMA+A + +A F Y + DG L EG E A H L L Sbjct: 120 GQGIANAFGMAVAEAYL-NARFGDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKL 178 Query: 172 ICLVDINNQQADGPSGKIMGFEPLADKWASFGWHVQRVDGNDLPAVIAAFDQARQLPESK 231 L D +NQ D ++ E +A+++ GWHV VDG+D+ AV A D A+ + Sbjct: 179 TLLWD-DNQITDDGKTELSISENVAERFRVAGWHVVEVDGHDIQAVSRALDAAQG--SDR 235 Query: 232 PRVILFDTLMGKGVPFLEQREKNHFIRVDPPEWQQALNIL 271 P +I T++G+G+P ++ + H R+ + Q A +L Sbjct: 236 PSMIACRTVIGRGIPRVQGQRGGHSARLYEDDAQAARELL 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 941,685 Number of extensions: 41642 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 4 Length of query: 281 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 197 Effective length of database: 1,222,130 Effective search space: 240759610 Effective search space used: 240759610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)