BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0156.p (298 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 128 5e-31 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 110 1e-25 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 100 3e-22 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 87 2e-18 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 128 bits (322), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 94/292 (32%), Positives = 144/292 (49%), Gaps = 8/292 (2%) Query: 9 TIGFIGTGIMGFPMARHLAQAGYRVQAWNRTPDKARRLSAFGAEVIAEAGDVIRDADALV 68 I FIG G MG PMA++L +AG+ V + +L G + A++ + AD ++ Sbjct: 4 NIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVI 63 Query: 69 CMLSSGPDCDALLLGEQGLIAGMRAGSLLVVMSSIPVETARHQAQQAAARGVAYVDAPVS 128 ML + ++L LGE G++A + G+LL+ S+I E AR A A ARG +DAPVS Sbjct: 64 TMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVS 123 Query: 129 GGEKGAIEASLAIMAGGTVQAYEQALPLLEKMGRPT-HVGPAGSGELAKLVNQMMVANNI 187 GG GA +L M GG+ +A+E A P L+KMG+ H G +GSG+ K+ N M++ + Sbjct: 124 GGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILM 183 Query: 188 ASVAEALLLAERGGADPAKVREALLGGFADSTILRQH-------ALRMISGDFAPGGPAR 240 +EA+ L G DP + E + + L + S +A G Sbjct: 184 IGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGFGVD 243 Query: 241 YQVKDTGTAIRFAASVGLDLPMLNKADRLFSDMLDHGDGDLDHSGVIRELRR 292 +KD G A+ A + +P+ A L+ +G G LD S + L + Sbjct: 244 LMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNMLGK 295 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 110 bits (275), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 74/283 (26%), Positives = 136/283 (48%), Gaps = 2/283 (0%) Query: 8 KTIGFIGTGIMGFPMARHLAQAGYRVQAWNRTPDKARRLSAFGAEVIAEAGDVIRDADAL 67 + +G IG G MG+ +A L +AG+ V A + + + +A G A ++ D + Sbjct: 3 RNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVV 62 Query: 68 VCMLSSGPDCDALLLGEQGLIAGMRAGSLLVVMSSIPVETARHQAQQAAARGVAYVDAPV 127 + ++ + + +L G+ G ++ M+ GS+++ +++ + A ++ +G+ +DAPV Sbjct: 63 ITLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPV 122 Query: 128 SGGEKGAIEASLAIMAGGTVQAYEQALPLLEKM-GRPTHVGPA-GSGELAKLVNQMMVAN 185 SGG A + +M G + Y + +L M G+ +G G G K++NQ++ Sbjct: 123 SGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGV 182 Query: 186 NIASVAEALLLAERGGADPAKVREALLGGFADSTILRQHALRMISGDFAPGGPARYQVKD 245 +IA+ AEA+ L R G +P + E + +S + ++SGD+ P VKD Sbjct: 183 HIAASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFVKD 242 Query: 246 TGTAIRFAASVGLDLPMLNKADRLFSDMLDHGDGDLDHSGVIR 288 G + A LP+ A ++F G G D S VI+ Sbjct: 243 LGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIK 285 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 99/287 (34%), Positives = 141/287 (49%), Gaps = 4/287 (1%) Query: 10 IGFIGTGIMGFPMARHLAQAGYRVQAWNRTPDKARRLSAFGAEVIAEAGDVIRDADALVC 69 I F+G G MGFPMA +L +AGY V+AWNR+P RL+A GA+ A + DAD L+ Sbjct: 3 IAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADADVLIS 62 Query: 70 MLSSGPDCDALLLGEQGLIAGMRAGSLLVVMSSIPVETARHQAQQAAARGVAYVDAPVSG 129 ML+ D L + G +A +R G++ V M++I V A A ARGVAYV APV G Sbjct: 63 MLAD-DDATETSLLDAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVLG 121 Query: 130 GEKGAIEASLAIMAGGTVQAYEQALPLLEKMGRPT-HVG-PAGSGELAKLVNQMMVANNI 187 A L I+A G QA PLL+ +G+ T +G AKL M+A+ I Sbjct: 122 RVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIASAI 181 Query: 188 ASVAEALLLAERGGADPAKVREALLGGFADSTILRQHALRMISGDFAPGG-PARYQVKDT 246 +++EA+ L + G D A E + + + + + F P G +KD Sbjct: 182 GAMSEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIADDRFEPAGFKLALGLKDV 241 Query: 247 GTAIRFAASVGLDLPMLNKADRLFSDMLDHGDGDLDHSGVIRELRRR 293 A+ + L + + L D L HG+G LD + + R RR Sbjct: 242 RLALEAGEQAHVPLSLASALRDLHIDGLAHGEGHLDWAALSRASARR 288 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 74/267 (27%), Positives = 125/267 (46%), Gaps = 2/267 (0%) Query: 7 IKTIGFIGTGIMGFPMARHLAQAGYRVQAWNRTPDKARRLSAFGAEVIAEAGDVIRDADA 66 +K IG IG G MG MA L + GY V ++ L+ G + + + Sbjct: 1 MKKIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEM 60 Query: 67 LVCMLSSGPDCDALLLGEQGLIAGMRAGSLLVVMSSIPVETARHQAQQAAARGVAYVDAP 126 +V L + + ++ GE GL+ G ++V ++ + R A + A G+ VDAP Sbjct: 61 VVLSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAP 120 Query: 127 VSGGEKGAIEASLAIMAGGTVQAYEQALPLLEKMG-RPTHVGPAGSGELAKLVNQMMVAN 185 VSGG KGA A++ ++ GG+ + + P+L M + HVGP G+G +AKL+N ++ Sbjct: 121 VSGGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVTGT 180 Query: 186 NIASVAEALLLAERGGADPAKVREALLGGFADSTILRQHALRMISGD-FAPGGPARYQVK 244 ++ EA +A G D ++ + L G A+S ++ I + F G + K Sbjct: 181 HLLIAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMRK 240 Query: 245 DTGTAIRFAASVGLDLPMLNKADRLFS 271 D A+ P+ +A RL++ Sbjct: 241 DLRLAMNLIEETRSTTPLAREAGRLWA 267 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 857,997 Number of extensions: 35120 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 4 Length of query: 298 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 213 Effective length of database: 1,217,395 Effective search space: 259305135 Effective search space used: 259305135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)