BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0152.p (306 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310443|ref|YP_003774535.1| LysR family transcription regul... 172 3e-44 gi|300309845|ref|YP_003773937.1| LysR family transcription regul... 167 7e-43 gi|300314051|ref|YP_003778143.1| transcription regulator protein... 161 7e-41 gi|300312645|ref|YP_003776737.1| LysR family transcription regul... 159 3e-40 gi|300312894|ref|YP_003776986.1| LysR family transcription regul... 126 2e-30 >gi|300310443|ref|YP_003774535.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 295 Score = 172 bits (436), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 105/286 (36%), Positives = 158/286 (55%), Gaps = 6/286 (2%) Query: 4 PLRSISVFHAVARSGSISSAAEELNVTPSAVSQQIQALETHLGTSLMVKVGRGVALTEAG 63 PL ++ F AVAR S + AA EL++T SAVS Q+ LE++LG L + GV +T AG Sbjct: 6 PLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVRITTAG 65 Query: 64 ERYFSMIAEEIDRISEATQSIRGLRPVTLLTVRATPTLSSKWLLPRLRSFLDAHPDLEVR 123 E Y + I I I+ A+ +R TL + + P+++S WL+ RL F ++P++ Sbjct: 66 EAYLARIQGAISAITAASMDVRQGVSNTLY-IHSAPSIASLWLMARLNDFARSYPEITFN 124 Query: 124 LNGTNEVTDFTRETVDVEIRHGRGQWPGLFTEGLTEEWFFPVCSPGYAAAGSM-SVADVA 182 L+ + ++F VD++IR+G WPGL E L EE P+ SP + + AD+ Sbjct: 125 LSAAHSQSNFELGEVDIDIRYGIPNWPGLVVEPLFEERIVPLASPAFIREYRLRKPADLL 184 Query: 183 QHRLIQSVKSQVQWPYWFS-MSKITPPNQMRRVLFDRSHMAVDAAADGIGLALESSLMMW 241 +LI+S S VQW WF + + P Q V FDR+ M++DAA G+G+ALES+ + Sbjct: 185 GVKLIRSNVSVVQWADWFGEFADVRAPEQF-AVRFDRAQMSLDAATQGLGVALESATVAA 243 Query: 242 RELRDGNLVCPVRNPPR-ISQTTQWIVCPVDHLRKNKVSVFIDWLR 286 R L + L P+ R I + V P H R+ V+ F++WLR Sbjct: 244 RHLEEKKL-KPIFGLERAIKVKAHFAVYPAHHARRPAVAAFLEWLR 288 >gi|300309845|ref|YP_003773937.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 167 bits (424), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 102/292 (34%), Positives = 157/292 (53%), Gaps = 8/292 (2%) Query: 1 MYLPLRSISVFHAVARSGSISSAAEELNVTPSAVSQQIQALETHLGTSLMVKVGRGVALT 60 M PL S+ F A+AR S + AA EL++TPSA+S Q+ LE LG L + +GV LT Sbjct: 1 MIPPLTSLMAFEAIARRKSFNLAAAELHLTPSAISHQVARLEKLLGLRLFERSTKGVELT 60 Query: 61 EAGERYFSMIAEEIDRISEATQSIR-GLRPVTLLTVRATPTLSSKWLLPRLRSFLDAHPD 119 AG+ Y + +A + I+ AT +R G+ L + ++P+ +S WL+PR+ F HPD Sbjct: 61 PAGQSYLNRVAGALGAINSATDDLRHGVE--NSLQIHSSPSFASLWLMPRIGRFTQRHPD 118 Query: 120 LEVRLNGTNEVTDFTRETVDVEIRHGRGQWPGLFTEGLTEEWFFPVCSPGYAAAGSMSVA 179 + + L+ + E++DF VD++IR G WP L E + E P+ SP + A + Sbjct: 119 IALGLSASPEISDFEIGQVDLDIRCGVPHWPDLEIEAVLSERVIPMASPDFLARHRIDTP 178 Query: 180 -DVAQHRLIQSVKSQVQWPYWFSMSKITPPNQMRRVL--FDRSHMAVDAAADGIGLALES 236 D+ LIQS S +QWP WF ++ P + R+ FDR+ M+++AA G+G+ ES Sbjct: 179 DDLVLAPLIQSRGSLLQWPEWF--ARYAPDLRPERIALRFDRAMMSIEAAVQGLGVVFES 236 Query: 237 SLMMWRELRDGNLVCPVRNPPRISQTTQWIVCPVDHLRKNKVSVFIDWLRVE 288 L G L + + T + VCP + + +V +F+ WLR E Sbjct: 237 DAFGAVALASGRLQPVFADGLALEITQHYAVCPARNKSRPEVMLFLQWLREE 288 >gi|300314051|ref|YP_003778143.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 306 Score = 161 bits (407), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 94/283 (33%), Positives = 158/283 (55%), Gaps = 9/283 (3%) Query: 20 ISSAAEELNVTPSAVSQQIQALETHLGTSLMVKVGRGVALTEAGERYFSMIAEEIDRISE 79 S AAEE++VTP A+S Q++ALE LG L ++ G+ +++TE G+R+ + I + + I+ Sbjct: 25 FSRAAEEVHVTPGAISHQVRALEEDLGLQLFLRHGKRISITETGQRFAATIRKSLSEIAL 84 Query: 80 ATQSIRGLRPVTLLTVRATPTLSSKWLLPRLRSFLDAHPDLEVRLNGTNEVTDFTRETVD 139 A + +R L + TP+ +S+WL PRL F+DAHP+LEV L + D + +D Sbjct: 85 AAEHLRSQNRQQRLVISTTPSFASRWLAPRLWRFIDAHPELEVVLQSAAHLNDLRLDGID 144 Query: 140 VEIRHGRGQWPGLFTEGLTEEWFFPVCSPGYAAAGSMSV---ADVAQHRLIQSVKSQVQW 196 V +R GRG + GL +E L++++++PV +PGY S + AD+A L++ V S+ W Sbjct: 145 VGLRFGRGNYSGLVSEKLSDDYYYPVAAPGYRGKRSKKLARPADLAHCSLLR-VDSRESW 203 Query: 197 PYWFSMSKITPPNQMRRVLFDRSHMAVDAAADGIGLALESSLMMWRELRDGNLV--CPVR 254 W + + + P +L + S + + AA+G G+AL ++ +E+ G LV V Sbjct: 204 QPWLAEAGLELPEPTGGLLVEDSSLTLRQAAEGKGVALARHVIASQEISTGELVRLFDVV 263 Query: 255 NPPRISQTTQWIVCPVDHLRKNKVSVFIDWLRVEVAQWQAEQA 297 P S+ + V + L K V+ F +W+ E+ + A A Sbjct: 264 VP---SEDAHYFVHLPEALEKPGVAAFKEWIFEEMRLFHASAA 303 >gi|300312645|ref|YP_003776737.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 287 Score = 159 bits (402), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 104/286 (36%), Positives = 147/286 (51%), Gaps = 5/286 (1%) Query: 1 MYLPLRSISVFHAVARSGSISSAAEELNVTPSAVSQQIQALETHLGTSLMVKVGRGVALT 60 MYLPL ++ F AR S + AAEEL+VT +AVS Q++ LE LG +L ++ RG+ALT Sbjct: 1 MYLPLNALRAFEVSARHLSFTRAAEELSVTQTAVSMQVKNLEQRLGVALFRRLPRGLALT 60 Query: 61 EAGERYFSMIAEEIDRISEATQSIRGLRPVTLLTVRATPTLSSKWLLPRLRSFLDAHPDL 120 + G ++A+ RI++ R +LTV T + WL+PRLR F HP + Sbjct: 61 DEGLALLPVVAQSFGRIADVLAQFEDGRRREVLTVGVVGTFAVGWLMPRLRQFQQQHPFV 120 Query: 121 EVRLNGTNEVTDFTRETVDVEIRHGRGQWPGLFTEGLTEEWFFPVCSPGYAAAGSMSVAD 180 ++RL N D E +D IR G G W G E L E P+C+P AA S AD Sbjct: 121 DLRLLTNNNRVDLAGEGLDYAIRFGDGAWHGTQAERLFEAPLAPMCAPAIAAR-LRSPAD 179 Query: 181 VAQHRLIQSVKSQVQWPYWFSMSKITPPNQMRRVLFDRSHMAVDAAADGIGLALESSLMM 240 + L++S +S +W WF+ + P +R +FD S +AAA G+AL LM Sbjct: 180 LGAETLLRSYRSD-EWGRWFAAAGAACPP-LRGFVFDSSLALAEAAAQEAGVALLPVLMF 237 Query: 241 WRELRDGNLVCPVRNPPRISQTTQWIVCPVDHLRKNKVSVFIDWLR 286 REL+ G LVCP RI W+ + ++ F WL+ Sbjct: 238 ERELQQGRLVCPFGQ--RIVLGAYWLTSLKSRPQTAAMAAFRQWLQ 281 >gi|300312894|ref|YP_003776986.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 89/283 (31%), Positives = 133/283 (46%), Gaps = 2/283 (0%) Query: 7 SISVFHAVARSGSISSAAEELNVTPSAVSQQIQALETHLGTSLMVKVGRGVALTEAGERY 66 S+ F A AR GS + AAEEL +T A+S+QI LE LG +L ++G V L G+RY Sbjct: 10 SLMAFEAAARHGSFARAAEELALTEGAISRQISRLEGFLGVTLFERIGNRVRLLPHGQRY 69 Query: 67 FSMIAEEIDRISEATQSIRGLRPVTL-LTVRATPTLSSKWLLPRLRSFLDAHPDLEVRLN 125 S + E ++R+ +Q + P L + +PT + +WL+PRL F HP + V L+ Sbjct: 70 ASHVRETLERLDRDSQHLMSQPPNGASLDIAVSPTFAMRWLIPRLPRFAALHPHITVHLS 129 Query: 126 GTNEVTDFTRETVDVEIRHGRGQWPGLFTEGLTEEWFFPVCSPGYAAAGSMSVADVAQHR 185 E +D I W G+ T L +E PVC P A S A A R Sbjct: 130 ERMEPFLLPGSGLDAAIHFLHPAWTGMQTYSLLDEVLVPVCHPALVAGTSGVPALDALPR 189 Query: 186 LIQSVKSQVQWPYWFSMSKITPPNQMRRVLFDRSHMAVDAAADGIGLALESSLMMWRELR 245 L ++ W + + + I+ + + +D M ++AA G+G+AL L + EL Sbjct: 190 L-HRRQNHDAWQRYAAQTGISLSHSAQGPRYDLHGMLIEAALAGLGVALVPRLYVETELA 248 Query: 246 DGNLVCPVRNPPRISQTTQWIVCPVDHLRKNKVSVFIDWLRVE 288 G LV P RIS+T ++ L + F W+ E Sbjct: 249 QGRLVAPWPQDSRISKTFCLVLPEPLQLGSEPLKAFAQWILEE 291 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 901,192 Number of extensions: 34497 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 53 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 53 Length of query: 306 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 221 Effective length of database: 1,217,395 Effective search space: 269044295 Effective search space used: 269044295 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)