BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0149.p (225 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 224 4e-60 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 205 2e-54 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 114 6e-27 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 99 2e-22 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 70 1e-13 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 224 bits (571), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 111/188 (59%), Positives = 141/188 (75%), Gaps = 2/188 (1%) Query: 27 GIAVLRGNLAPRGAIIKQSAADARLMEHEGRAVVFENVEDMATRIDSDALDVNADDILVL 86 GIA+L+GNLAP GA+IK SAA L++H GRAVVFEN +D+ RID + LDV+ +LVL Sbjct: 378 GIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVL 437 Query: 87 KNIGPKGAPGMPEAGYIPIPRKLARAGVKDIVRISDGRMSGTAFGTIVLHVTPESAIGGP 146 KN GPKG PGM EAG +P+P K+ R G+ D+VR+SD RMSGTA+GT+VLHV+PE+A GGP Sbjct: 438 KNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGP 497 Query: 147 LAYVQDGDRIRLSVANREIALLVADEELARR--VQETPVVIPSAGRGYAKLFLDTVTQAD 204 LA VQ+GD I L V R++ L V+DEELARR + P + P RG+ KL++D V QA+ Sbjct: 498 LAVVQNGDFIELDVEARKLHLDVSDEELARRRAQWQKPELPPQMQRGWVKLYVDHVQQAN 557 Query: 205 QGVDFDFL 212 QG D DFL Sbjct: 558 QGADLDFL 565 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 205 bits (522), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 106/199 (53%), Positives = 136/199 (68%), Gaps = 7/199 (3%) Query: 21 LAXP---QGGIAVLRGNLAPRGAIIKQSAADARLMEHEGRAVVFENVEDMATRIDSDALD 77 LA P GGI +LRGNLAPRGA++K SAA LM+H GRAVVFE+ RI+ LD Sbjct: 374 LAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLD 433 Query: 78 VNADDILVLKNIGPKGAPGMPEAGYIPIPRKLARAGVKDIVRISDGRMSGTAFGTIVLHV 137 V+A +LV+KN+GPKG PGM E G + +P K+ GVKD+VRISD RMSGTA+GT++LHV Sbjct: 434 VDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDARMSGTAYGTVILHV 493 Query: 138 TPESAIGGPLAYVQDGDRIRLSVANREIALLVADEELARRVQETPVVI----PSAGRGYA 193 PE+A GGPL VQDGD I L ++ L +++EE+ RR++ V+ P GY Sbjct: 494 APEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKVLAERKPEMVGGYQ 553 Query: 194 KLFLDTVTQADQGVDFDFL 212 L++D V QAD+G DFDFL Sbjct: 554 SLYVDRVLQADEGCDFDFL 572 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 114 bits (285), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 73/208 (35%), Positives = 111/208 (53%), Gaps = 14/208 (6%) Query: 25 QGGIAVLRGNLAPRGAIIKQSAADARLMEH-----------EGRAVVFENVEDMATRIDS 73 + G VL+GNL AI+K S E E RAVVF+ +D RI+ Sbjct: 382 RAGFLVLKGNLFDF-AIMKTSVISETFRERYLSTPGQENIFECRAVVFDGSDDYHARIND 440 Query: 74 DALDVNADDILVLKNIGPKGAPGMPEAGYIPIPRKLARAGVKDIVRISDGRMSGTAFGTI 133 AL ++ + +L ++ GP G PG E + P L + GV + + DGR SGT+ Sbjct: 441 PALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLPTLGDGRQSGTSDSPS 500 Query: 134 VLHVTPESAIGGPLAYVQDGDRIRLSVANREIALLVADEELARRVQETPVVIPSAGRGYA 193 +L+ +PESA+GG LAY++DGDR+R+ + E +LV++EELARR E +P + + Sbjct: 501 ILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELARRKSEGIPPVPPSQTPWQ 560 Query: 194 KLFLDTVTQADQGVDFDFLRAVKMVGKA 221 +++ TV Q + G + A+K G A Sbjct: 561 EIYRSTVGQLETGACMEL--ALKYQGVA 586 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 99.4 bits (246), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 56/152 (36%), Positives = 89/152 (58%), Gaps = 3/152 (1%) Query: 26 GGIAVLRGNLAPRGAIIKQSAADARLMEHEGRAVVFENVEDMATRIDSDALDVNADDILV 85 GG+AVL GN+A +G I+K + D +++ G A VFE+ + I D V A D+++ Sbjct: 424 GGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGD--KVKAGDVVI 481 Query: 86 LKNIGPKGAPGMPEAGYIPIPRKLARAGVKDIVRISDGRMSGTAFGTIVLHVTPESAIGG 145 ++ GPKG PGM E Y P ++ K +DGR SG + G ++ H +PE+A GG Sbjct: 482 VRYEGPKGGPGMQEMLY-PTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGG 540 Query: 146 PLAYVQDGDRIRLSVANREIALLVADEELARR 177 + V++GD I + + NR I L ++ ++LA+R Sbjct: 541 AIGLVEEGDMIDIDIPNRTINLRISSDDLAKR 572 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 70.1 bits (170), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 70/205 (34%), Positives = 99/205 (48%), Gaps = 14/205 (6%) Query: 10 AQGGIAVLRGNLAXP---QGGIAVLRGNLAPRGAIIKQSAADARLMEHEGRAVVFENVED 66 AQ G + G +A P GG+ +L+GNL A+IK SA E AVVF + E Sbjct: 408 AQSGDEGVLGTVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQEA 465 Query: 67 MATRIDSDALDVNADDILVLKNIGPKGAPGMPEAGYIPIPRKLARAGVKDIVRISDGRMS 126 + LD D + V+ GP+ A GMPE + + + + + ++DGRMS Sbjct: 466 FMAAFKAGQLD--RDFVAVITFQGPR-ANGMPELHALTPALGILQDKGRHVALVTDGRMS 522 Query: 127 GTAFGTI--VLHVTPESAIGGPLAYVQDGDRIRLSVANREIALLVADEELARRVQETPVV 184 G A G + +HVTPE GGPL V+DGD IRL + V E A R Q++ + Sbjct: 523 G-ASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAARSQDSSDL 581 Query: 185 IPSA---GRGYAKLFLDTVTQADQG 206 + GR LF + V A++G Sbjct: 582 SGNQHGMGRELFSLFRNAVGTAEEG 606 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 746,034 Number of extensions: 32270 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 5 Length of query: 225 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 143 Effective length of database: 1,231,600 Effective search space: 176118800 Effective search space used: 176118800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)