BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0145.p (292 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 481 e-137 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 123 2e-29 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 100 3e-22 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 87 2e-18 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 81 1e-16 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 481 bits (1239), Expect = e-137, Method: Compositional matrix adjust. Identities = 229/255 (89%), Positives = 242/255 (94%) Query: 1 MQYRPLGRTNVNVSLITLGTMTWGEQNTEAEAHAQLDYATERGINLIDVAEMYPVPPKAE 60 MQYRPLGRT++ VSLITLGTMTWGEQN+EA+AHAQLDYA ERGINLIDVAEMYPVPPKAE Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 61 TQGLSERYLGTWLKKSGKRDQLLIATKATGPARKPHNPRHVRGGINNFDRKGLTEALNGS 120 TQGL+ERYLGTWLKKSGKRDQLLIATK TGPARKPHNPRHVRGGINN DRKGLTEAL+GS Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGS 120 Query: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLNYSHIADEETVAIEETLSILSEFVKAGKIRHIG 180 LERLQLDHVDLYQLHWPDRSVNSFGQL Y H+ DE TV IEETL++LSEFVKAGKIR IG Sbjct: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIG 180 Query: 181 LSNETSWGVAQFLRAAEKFDLERIVTIQNPYNLLNRTFEINLAEFAHREQVGLLAYSPLA 240 LSNET+WGVAQFLRAAE+ LERIVTIQNPYNLLNR+FEINLAEFAHREQVGLLAYSPLA Sbjct: 181 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA 240 Query: 241 FGMLSGKYENGARPA 255 FGMLSGKY +GARPA Sbjct: 241 FGMLSGKYLDGARPA 255 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 80/262 (30%), Positives = 132/262 (50%), Gaps = 35/262 (13%) Query: 1 MQYRPLGRTNVNVSLITLGTMTWGEQNTEAEAHAQLDYATERGINLIDVAEMYPVPPKAE 60 M YR LG++N+ VS + LG+M +G+Q E+ + A E G+N ID A++Y Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVY------- 53 Query: 61 TQGLSERYLGTWLKKSGKRDQLLIATK-ATGPARKPHNPRHVRGGINNFDRKGLTEALNG 119 T+G SE +G L +G+R + ++A+K + KP+ +N+ R + Sbjct: 54 TKGKSEEIVGKLL--AGQRHEWVLASKLGNAMSGKPNE--------SNYSRSWIVRETEN 103 Query: 120 SLERLQLDHVDLYQLHWPDRSVNSFGQLNYSHIADEETVAIEETLSILSEFVKAGKIRHI 179 L RL D++D+ +H D + +EE + L + ++ GKIR Sbjct: 104 ILTRLDTDYLDILYMH-----------------RDYQDENLEEAVQALGDLIRVGKIRGF 146 Query: 180 GLSNETSWGVAQFLRAAEKFDLERIVTIQNPYNLLNRTFEINLAEFAHREQVGLLAYSPL 239 G+SN W +A+ +R EK ++ + Q YNLLNR E+ + +G++ YSP+ Sbjct: 147 GVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPI 206 Query: 240 AFGMLSGKYENGARPAAPWTTG 261 A G+L+GKY+ G +PA G Sbjct: 207 ARGVLTGKYQPGQKPAQDTRAG 228 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 85/252 (33%), Positives = 123/252 (48%), Gaps = 45/252 (17%) Query: 4 RPLGRTNVNVSLITLGTMTWGE---QNTEAEAHAQLDYATERGINLIDVAEMY-PVPPKA 59 R LG VS I LG M + Q +AE+ A +D A E GINL+D A+MY P Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPY---- 73 Query: 60 ETQGLSERYLGTWLKKSGKRDQLLIATKATGPARKPHNP--RHVRGGINNFDRKGLTEAL 117 +E LG +K G+RD+ IATK G R P +P R V G + +A+ Sbjct: 74 ----TNEELLGRAIK--GRRDKFFIATK-FGIRRDPSDPGARGVDGSPAY-----IRQAV 121 Query: 118 NGSLERLQLDHVDLYQLHWPDRSVNSFGQLNYSHIADEETVAIEETLSILSEFVKAGKIR 177 GSL+RL ++ +DLY Y H D T IE T+ +L++ V+AGKIR Sbjct: 122 EGSLKRLGVETIDLY----------------YQHRIDPAT-PIEVTMGVLADLVQAGKIR 164 Query: 178 HIGLSNETSWGVAQFLRAAEKFDLERIVTIQNPYNLLNRTFEINLAEFAHREQVGLLAYS 237 +IGLS ++ + + R + + +Q+ Y+L R E + +G +AYS Sbjct: 165 YIGLSEASAATLERAHR------VHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYS 218 Query: 238 PLAFGMLSGKYE 249 PL G L+G + Sbjct: 219 PLGRGFLTGAIQ 230 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 82/251 (32%), Positives = 121/251 (48%), Gaps = 42/251 (16%) Query: 1 MQYRPLGRTNVNVSLITLGTM----TWGEQNTEAEAHAQLDYATERGINLIDVAEMYPVP 56 M+ R GRT VS I G +WG+ + + +A A L A + G++ ID A++Y Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQ-DAKAALHAALDAGVSFIDTADVY--- 56 Query: 57 PKAETQGLSERYLGTWLKKSGKRDQLLIATKATGPARKPHNPRHVRGGINNFDRKGLTEA 116 G SE+ + LK+ G ++ +ATKA G PH V G N DR L+ Sbjct: 57 ----GDGRSEQLIAQVLKERGG-ERPFVATKA-GRRLSPH----VASGYN--DRAQLSAF 104 Query: 117 LNGSLERLQLDHVDLYQLHWPDRSVNSFGQLNYSHIADEETVAIEETLSILSEFVKAGKI 176 ++ SL+ L +D +DL QLH P V + Q + H L V AGKI Sbjct: 105 IDRSLKNLGVDCLDLVQLHCPPTEVY-YQQEVFGH---------------LDALVAAGKI 148 Query: 177 RHIGLSNETSWGVAQFLRAAEKFDLERIVTIQNPYNLLNRTFEINLAEFAHREQVGLLAY 236 RH G+S E V + L+A E +++ + I N + R + L E A R + ++A Sbjct: 149 RHYGVSVEK---VEEALKAIEYPNVKSVQIIFNIFR--QRPISLFLQE-AQRRDIAVIAR 202 Query: 237 SPLAFGMLSGK 247 PLA G+L+GK Sbjct: 203 VPLASGLLTGK 213 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 80.9 bits (198), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 78/261 (29%), Positives = 117/261 (44%), Gaps = 46/261 (17%) Query: 1 MQYRPLGRTNVNVSLITLGT-MTWGEQNTEAEAHAQLDYATERGINLIDVAEMYPVPPKA 59 M YR GR+ + + I+LG +G + A L A +RG+ D+A Y P Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPP--- 70 Query: 60 ETQGLSERYLGTWLKKS--GKRDQLLIATKAT-----GPARKPHNPRHVRGGINNFDRKG 112 G +E G W+ K RD+L+I++KA GP P R K Sbjct: 71 --AGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGAR-----------KH 117 Query: 113 LTEALNGSLERLQLDHVDLYQLHWPDRSVNSFGQLNYSHIADEETVAIEETLSILSEFVK 172 L + SL+R+ LD+VD++ YSH D +T +EET+ L+ + Sbjct: 118 LIASCEQSLKRMGLDYVDIF----------------YSHRVDADT-PLEETMGALAHLHR 160 Query: 173 AGKIRHIGLSNETSWGVAQFLRAAEKFDLERI-VTIQNP-YNLLNRTFEINLAEFAHREQ 230 GK ++G+S S+ +AA E + + I P Y++LNR E L Sbjct: 161 QGKALYVGIS---SYSPELTRKAAAILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELG 217 Query: 231 VGLLAYSPLAFGMLSGKYENG 251 VG + +SPLA G+L+ KY G Sbjct: 218 VGCIGFSPLAQGLLTSKYLGG 238 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 874,378 Number of extensions: 35181 Number of successful extensions: 102 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 94 Number of HSP's successfully gapped: 6 Length of query: 292 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 208 Effective length of database: 1,222,130 Effective search space: 254203040 Effective search space used: 254203040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)