BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0140.p (295 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 445 e-126 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 159 2e-40 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 77 1e-15 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 445 bits (1145), Expect = e-126, Method: Compositional matrix adjust. Identities = 218/239 (91%), Positives = 233/239 (97%) Query: 57 NKPVNHHMGVSAFADHAVVSRRSCVKIEADIDPVEAALFGCAVLTGVGAAVNTAEVKAGT 116 +PV+HH+GVSAFADHAVVSRRSCVKIEADIDPV+AALFGCAVLTGVGAAVNTAEVKAGT Sbjct: 136 TQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAEVKAGT 195 Query: 117 TAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALAKSLGATATVNARDPDAVEKVR 176 TAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALA+SLGATATVNARDPDAVEKVR Sbjct: 196 TAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVEKVR 255 Query: 177 ALTKGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPDQKWALQQVSLVAEERTVK 236 ALT+GGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAP Q WALQQVSLVAEERTVK Sbjct: 256 ALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEERTVK 315 Query: 237 GSYIGSCVPLRDIPRYIGLYKSGRLPIDKLMGQRLALSDINSGFDRLASGEGLRDLIVF 295 GSYIGSCVP+RDIPRYIGL+++G+LP+D+LMG+RLAL+DIN GFDRL GEGLRDLIVF Sbjct: 316 GSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFDRLHGGEGLRDLIVF 374 Score = 96.3 bits (238), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 49/74 (66%), Positives = 57/74 (77%), Gaps = 3/74 (4%) Query: 1 MKIQAAVLRETTTVEPFASSRPLRIEELDLDAPGPGEVLVKMRAAGLCHSDLSVIXN--- 57 MKIQAAVLRE + PFA S+PL+IEELDLD PGPGEVLV+M+AAGLCHSDLSVI Sbjct: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 Query: 58 KPVNHHMGVSAFAD 71 +PV +G A A+ Sbjct: 61 RPVPMALGHEASAE 74 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 159 bits (403), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 88/240 (36%), Positives = 134/240 (55%), Gaps = 3/240 (1%) Query: 58 KPVNHHMGVSAFADHAVVSRRSCVKIEADIDPVEAALFGCAVLTGVGAAVNTAEVKAGTT 117 KP+ H+MG S F+++ VV + KI +D + GC V TGVGA + TA+V+AG Sbjct: 130 KPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAVLFTAKVEAGAN 189 Query: 118 AAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALAKSLGATATVNARD-PDAVEKVR 176 V GLGG+GL + A GA ++I VDL+ ++ A+A+ G T +N +D P+ V+ + Sbjct: 190 VVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKDVPNVVDAII 249 Query: 177 ALTKGGVDYAFEMAGSVQAMEAAYRMTRRG-GMTVTAGLPAPDQKWALQQVSLVAEERTV 235 +T GG DY+FE G+ M + +G G ++ G+ A Q+ + + LV R Sbjct: 250 GMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPFQLVT-GRVW 308 Query: 236 KGSYIGSCVPLRDIPRYIGLYKSGRLPIDKLMGQRLALSDINSGFDRLASGEGLRDLIVF 295 KGS G D+P+ + Y G+L ID L+ +L L IN GFD + SGE +R ++ F Sbjct: 309 KGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSGESIRSVVEF 368 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 69/220 (31%), Positives = 111/220 (50%), Gaps = 11/220 (5%) Query: 68 AFADHAVVSRRSCVKIEADIDPVEAALFGCAVLTGVGAAVNTAEVKAGTTAAVLGLGGVG 127 +FA++ + + + ++D AA CA +T V + EV+ G + G+GG+G Sbjct: 123 SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVT-VYKGLKETEVRPGEWVVISGIGGLG 181 Query: 128 LCAMLGALASGAREVIAVDLHDSKLALAKSLGATATVNARDPDAVEKVRALTKGGVDYAF 187 A+ A A G V+A D+H+ KLALAK LGA TV+ R+ +AV +V+ + GG A Sbjct: 182 HMAVQYAKAMGMH-VVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRII-GGAHGAL 239 Query: 188 EMAGSVQAMEAAYRMTRRGGMTVTAGLPAPDQKWALQQVSLVAEERTVKGSYIGSCVPLR 247 A S +AME A+ R G GLP D +L + V + TV+GS +G+ + Sbjct: 240 VTAVSPKAMEQAFGFLRARGTMALVGLPPGDI--SLPVFNTVLKRITVRGSIVGT---RQ 294 Query: 248 DIPRYIGLYKSGRLPIDKLMGQRLALSDINSGFDRLASGE 287 D+ + G++ + L +IN+ F R+ G+ Sbjct: 295 DLEESLVFAAEGKVAAHFTWDK---LDNINAIFARMEEGK 331 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 819,167 Number of extensions: 32132 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 6 Length of query: 295 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 210 Effective length of database: 1,217,395 Effective search space: 255652950 Effective search space used: 255652950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)