BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0138.p (254 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 144 8e-36 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 129 3e-31 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 121 7e-29 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 91 1e-19 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 80 2e-16 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 144 bits (362), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 69/125 (55%), Positives = 86/125 (68%), Gaps = 6/125 (4%) Query: 4 DVTSATDVKAAVKHCVDRFGVPDIIVNNAGTTHRNQPMLDVDEATFDRMYAINVKSIYHM 63 DV+ D+ + ++ FG DI+VNNAGTTHRN+PML+V+E FDR+YA+NVKSI+ Sbjct: 62 DVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFLS 121 Query: 64 TRAVLPLMRERNAQQKDRGGVMLNIGSVAGIRPRPGLTWYNSSKGAVNMLSKSMAVELAP 123 + +P R+ GG +NI S AGIRPRPGLTWYN SKGAV SKSMA EL P Sbjct: 122 AKTFVPYFRQVG------GGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGP 175 Query: 124 DRIRV 128 D+IRV Sbjct: 176 DKIRV 180 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 129 bits (323), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 62/124 (50%), Positives = 88/124 (70%) Query: 128 VAEETGAQAVQMAHDGLTIDKILTPAAFENAMRVLLAIGGSTNGIVHLTAIAGRMGLEID 187 +A G + VQM + L + KILT AFENA+RV AIGGSTN ++HL AIAGR+G+++D Sbjct: 235 LARNAGRRIVQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLD 294 Query: 188 LDALDRMGRETPVLLDLKPSGKHYMEDFHHAGGMATLLRELKPLLRLEALTVTGRTLGEE 247 L D +G++ P LLDL+PSGK MEDF++AGG+ ++R+L+ ++ ALT G+TL E Sbjct: 295 LKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESVIDKTALTANGKTLWEN 354 Query: 248 IERA 251 + A Sbjct: 355 CQDA 358 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 121 bits (303), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 61/130 (46%), Positives = 92/130 (70%), Gaps = 3/130 (2%) Query: 125 RIRVAEETGAQAVQMAHDGLTIDKILTPAAFENAMRVLLAIGGSTNGIVHLTAIAGRMGL 184 R +A +G + V M +GLT+ KILT AFENA+R AIGGSTN ++HL AIAGR+G+ Sbjct: 234 RYVLAHLSGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGV 293 Query: 185 EIDLDALDRMGRETPVLLDLKPSGKHYMEDFHHAGGMATLLRELKP---LLRLEALTVTG 241 +++L+ R+GR TP ++DL+PSG++ ME+F++AGG+ +LR L L +ALTV G Sbjct: 294 DLELEDWTRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNG 353 Query: 242 RTLGEEIERA 251 +T+ + ++ A Sbjct: 354 QTMWDNVKDA 363 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 90.5 bits (223), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 51/128 (39%), Positives = 81/128 (63%), Gaps = 2/128 (1%) Query: 124 DRIRVAEETGAQAVQMAHDGLTIDKILTPAAFENAMRVLLAIGGSTNGIVHLTAIAGRMG 183 +R ++A ETG + V MA++ L ILT AF +A+ V AIGGSTN H+ A+A G Sbjct: 235 ERGQMAYETGRRIVGMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTNAQPHIMAMARHAG 294 Query: 184 LEIDLDALDRMGRETPVLLDLKPSGKHYMEDFHHAGGMATLLRELKPL--LRLEALTVTG 241 +E+ + + G + P+LL+++P+GK+ E FH AGG+ ++ EL+ LR E +T TG Sbjct: 295 VELQSEDWMKYGYDVPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATG 354 Query: 242 RTLGEEIE 249 +T+ E ++ Sbjct: 355 KTMAENLQ 362 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 48/127 (37%), Positives = 71/127 (55%), Gaps = 7/127 (5%) Query: 3 CDVTSATDVKAAVKHCVDRFGVPDIIVNNAGTTHRNQPMLDVDEATFDRMYAINVKSIYH 62 DV + + AAV V + G D+++NNAG + P+ V + FD + IN +S+ Sbjct: 63 ADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVA 122 Query: 63 MTRAVLPLMRERNAQQKDRGGVMLNIGSVAGIR-PRPGLTWYNSSKGAVNMLSKSMAVEL 121 TRAV+P+MR + GG ++N+ SVA PG Y +SKG V+ L++ MA EL Sbjct: 123 FTRAVIPVMRAQG------GGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKEL 176 Query: 122 APDRIRV 128 D+IRV Sbjct: 177 LADKIRV 183 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 721,455 Number of extensions: 28345 Number of successful extensions: 187 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 22 Length of query: 254 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 171 Effective length of database: 1,226,865 Effective search space: 209793915 Effective search space used: 209793915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)