BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0132.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314069|ref|YP_003778161.1| amino acid ABC transporter perm... 103 2e-23 gi|300312953|ref|YP_003777045.1| amino acid ABC transporter perm... 85 9e-18 gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC t... 84 1e-17 gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 75 1e-14 gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC t... 71 1e-13 >gi|300314069|ref|YP_003778161.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 326 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 87/250 (34%), Positives = 138/250 (55%), Gaps = 10/250 (4%) Query: 45 SFGIAALGVSILI-RSGQLSFGHAMFSCAAGYAVAFAARAMPELDSAVLILLGTVFGAVM 103 F + A ++LI +G LSFGHA F AGY A + L + + +++GT+ GA++ Sbjct: 31 CFALFACAFNLLIGYTGLLSFGHAAFLGWAGYMCGQALKVW-GLPTELGLIIGTLTGALI 89 Query: 104 GMVIGLFVVRYRAIFFGMLNLALSMVLFSILGKFYSLTGGTDGIR-LARPTFFG-VRMER 161 G+V+GL +R + I+F M+ LAL+ +LF + + TGG DG++ + R G + +E Sbjct: 90 GLVMGLLAIRRQGIYFTMITLALAQMLFFVALQ-APFTGGEDGLQGVPRGKLLGFLNLES 148 Query: 162 DGFETTLLVVAVVLAVALAWGVQRYMMSIAGQALTAVKTNETRLEYVGLSANRALWIGYV 221 D TL V + +AVA + R + S GQ L A+K NE R +G R + +V Sbjct: 149 D---MTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVARYKLLAFV 205 Query: 222 ISAALCGLSGAMFALMQGLVTPEMGYWVRSGEIVFISILGGVGHPVGAFLGAVVFEFVKL 281 +SA L GL+GA L+ G T +W SG +V ++++GG+G +G +GAV+ + L Sbjct: 206 LSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLGTILGPIVGAVI--IIML 263 Query: 282 YSAALFTGAW 291 + GAW Sbjct: 264 ENKLGDIGAW 273 >gi|300312953|ref|YP_003777045.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 317 Score = 84.7 bits (208), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 88/263 (33%), Positives = 143/263 (54%), Gaps = 10/263 (3%) Query: 55 ILIRSGQLSFGHAMFSCAAGYAVAFAARAMPELDSAVLI-LLGTVFGAVMGMVIGLFVVR 113 IL G +SFGHA+F Y A A+ + SA LI LL + A++G+VIG+ +R Sbjct: 48 ILGYGGMVSFGHAVFFGLGAYVTAIAS--FHGVTSAWLIWLLAALVTALVGLVIGVIALR 105 Query: 114 YRAIFFGMLNLALSMVLFSILGKFYSLTGGTDGIRL-ARPTF-FGVRMERDGFETTLLVV 171 RA++F M+ +AL+ + + F GG DG+++ ARP FG+ M D VV Sbjct: 106 TRAVYFIMITMALAQLFYYFFVGF-RYYGGDDGLQVTARPLLGFGLDMSGD--NAFYWVV 162 Query: 172 AVVLAVALAWGVQRYMMSIAGQALTAVKTNETRLEYVGLSANRALWIGYVISAALCGLSG 231 LA + +QR + S G AL A++ NE R + +G A R +VI+A + G++G Sbjct: 163 LAWLAASFIL-LQRLLASRFGLALNAIRQNERRAQAIGYPALRYKLAAFVIAAVIAGIAG 221 Query: 232 AMFALMQGLVTPEMGYWVRSGEIVFISILGGVGHPVGAFLGAVVFEFVKLYSAALFTGAW 291 ++ + +P++ +W +SG ++ + LGGVG+ G LGA F ++ + F W Sbjct: 222 SLIGMSNLFASPKLLHWSQSGILLVMVALGGVGYFYGGLLGAAAFLLLEEVLSTHFE-YW 280 Query: 292 QMVLGFVLIGLVFFVPTGLSGLL 314 Q+ +G +L+ +V P G+S L+ Sbjct: 281 QIYVGLILLLVVMVAPRGVSSLV 303 >gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 408 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 80/334 (23%), Positives = 153/334 (45%), Gaps = 32/334 (9%) Query: 4 KITMSKTTSPKIKIAGVLLVVFALGLPMIWPWLKTP-----MVVATSFGIAALGVSILIR 58 K+ P+ + A V++++ A ++WP+ + M +A + + LG++I++ Sbjct: 68 KLPALPRMQPRQQRAAVMILLMA---ALVWPFFGSRGYVDVMTLALIYVVLGLGLNIVVG 124 Query: 59 -SGQLSFGHAMFSCAAGYAVAFAARAMPELDSAVLILLGTVFGAVMGMVIGLFVVRYRAI 117 +G L G+ F Y A + L + L A+ G ++G V+R R Sbjct: 125 FAGLLDLGYVGFYAVGAYTYALCNQYF-GLTFWECVPLAAAASALFGFLLGFPVLRLRGD 183 Query: 118 FFGMLNLALSMVLFSILGKFYSLTGGTDGIR-LARPTFFGVRMERD-------------- 162 + ++ L ++ +L S+TGG DG+ + +PT FG+ M R+ Sbjct: 184 YLAIVTLGFGEIIRLLLNNMTSITGGPDGVSGIPKPTVFGLVMARNPVTEGGTTFHQLFG 243 Query: 163 -----GFETTLLVVAVVLAVALAWGVQRYMMSIA-GQALTAVKTNETRLEYVGLSANRAL 216 G L +L V + V ++ + G+A A++ +E +G++ + Sbjct: 244 LSYQGGHMVIFLYFLALLMVLFTYFVTSRLLRMPMGRAWEALREDEIACRSLGINPTKVK 303 Query: 217 WIGYVISAALCGLSGAMFALMQGLVTPEMGYWVRSGEIVFISILGGVGHPVGAFLGAVVF 276 + + AA GL+G+ FA QGLVTPE ++ S I+ I +LGG+G +G L A++ Sbjct: 304 LSAFTLGAAFAGLAGSFFAARQGLVTPESFTFIESALILAIVVLGGMGSQLGVILAAILL 363 Query: 277 EFVKLYSAALFTGAWQMVLGFVLIGLVFFVPTGL 310 + + + F ++ G V++ ++ + P GL Sbjct: 364 TVLPELARS-FAEYRMLIFGLVMVLMMMWRPQGL 396 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 66/229 (28%), Positives = 112/229 (48%), Gaps = 23/229 (10%) Query: 60 GQLSFGHAMFSCAAGYAVAFAA-RAMPELDSAVLILLGTVFGAVMGMVIGLFVVRYRAIF 118 G +SFGHA + YA A R + A+L GT + ++ G F VR ++ Sbjct: 372 GMVSFGHAAYFGLGAYAAALVTLRLQWPMPLAMLAGAGTAM--LAALLFGWFCVRLSGVY 429 Query: 119 FGMLNLALSMVLFSILGKFYSLTGGTDGI-------RLARPTFFGVRMERDGFETTLLVV 171 ML LA + ++++ + ++ LTGG++G+ LA P + + LL Sbjct: 430 LAMLTLAFAQIVWAAIYQWDDLTGGSNGLVGLRTAAALASPLAY--------YYLALLCS 481 Query: 172 AVVLAVALAWGVQRYMMSIAGQALTAVKTNETRLEYVGLSANRALWIGYVISAALCGLSG 231 A L V L ++R + + G AL A + R + +G+ W G+ ++ CGL+G Sbjct: 482 A--LGVYL---LRRVIHAPFGLALRAARDASGRAQALGMDVRALQWGGFALAGLFCGLAG 536 Query: 232 AMFALMQGLVTPEMGYWVRSGEIVFISILGGVGHPVGAFLGAVVFEFVK 280 +FA +G ++PE+ RS + + + +LGGV G LGA VF +++ Sbjct: 537 VLFAFSKGSISPEVAGVNRSVDGLVMVLLGGVQSLAGPLLGASVFTWLQ 585 >gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 334 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 79/275 (28%), Positives = 138/275 (50%), Gaps = 6/275 (2%) Query: 46 FGIAALGVSILI-RSGQLSFGHAMFSCAAGYAVAFAARAMPELDSAVLILLGTVFGAVMG 104 F +AALG ++L+ +G +SFG +F Y A +L + L + GA++ Sbjct: 36 FALAALGCNLLLGYTGLMSFGQGIFFGVGSY-TAGVVLLQLKLALLPALALAALGGALVA 94 Query: 105 MVIGLFVVRYRAIFFGMLNLALSMVLFSILGKFYSLTGGTDGI-RLARP--TFFGVRMER 161 +++G F +R R +F ML LA + + + + +TGG +G+ + RP FG+ + Sbjct: 95 LLVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTMKDVTGGDNGLLDIPRPPLALFGLTLLP 154 Query: 162 DGFETTLLVVAVVLAVALAWGVQRYMMSIAGQALTAVKTNETRLEYVGLSANRALWIGYV 221 V +L V + W +QR + S+ G+ L AV+ NE R +G +V Sbjct: 155 TTSSWQYYTVVAILFVLVFWLLQRVVDSVLGKTLLAVRENEARASALGYDVRLLKLAAFV 214 Query: 222 ISAALCGLSGAMFALMQGLVTPEMGYWVRSGEIVFISILGGVGHPVGAFLGAVVFEFVKL 281 IS A+ GL+G + A+M G+ + S I+ ++++GG G+ + LGA + V Sbjct: 215 ISGAVTGLAGGLHAMMTGVAPLSNIEYHTSEAILVMTVIGGTGNLFASVLGASFYVLVGN 274 Query: 282 YSAALFTGAWQMVLGFVLIGLVFFVPTGLSGLLFR 316 + + L+ W M+LG +LI + ++ GL GL + Sbjct: 275 WLSTLWP-RWLMLLGLLLIAVSLYMQKGLFGLAMK 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.143 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 897,944 Number of extensions: 35325 Number of successful extensions: 300 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 285 Number of HSP's successfully gapped: 22 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)