BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0124.p (372 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 627 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 173 2e-44 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 161 9e-41 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 149 4e-37 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 104 1e-23 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 627 bits (1618), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/358 (87%), Positives = 340/358 (94%), Gaps = 1/358 (0%) Query: 1 MSLQSSSPDAYQDLRDAVRDLCSTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAE 60 MS ++S D++ DLRDAVRDLC TFPPEYWRK+DEARGYPE+FVDALTKAGWLAALIP E Sbjct: 1 MSYPTAS-DSHADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQE 59 Query: 61 YGGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSAEQKQLYLPKIASGELR 120 YGGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGS EQK+ YLP+IASGELR Sbjct: 60 YGGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELR 119 Query: 121 LQSMGVTEPTTGTDTTKIKTTAVRKGDRYVVNGQKVWISRIQHSDLMILLARTTPLDQVK 180 LQSM VTEPTTGTDTT+IKT AV+KGDRYV+NGQKVWISR+QHSDLMILLARTTPL+QV Sbjct: 120 LQSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVS 179 Query: 181 KKSEGMSIFLVDLKHAIANGMSVRPILNMVNHETNELFFDNLEIPVENLIGEEGKGFKYI 240 +KSEGMSIFLVDLK AI +GM VRPILNMVNHETNELFFDNLEIP ENLIGEEG+GF+YI Sbjct: 180 RKSEGMSIFLVDLKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYI 239 Query: 241 LDGLNAERTLIAAECIGDGYWFIEKAAQYAKERVVFDRPIGMNQGVQFPIADSYIELEAA 300 LDGLNAERTLIAAECIGDGYWFI+KA QYA+ERVVFDRPIGMNQGVQFP+A++YIE+EAA Sbjct: 240 LDGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAA 299 Query: 301 NLMRFKACELFDARQPCGAQANMAKFLAAKASWEAANVCLQTHGGFGFACEYDVERKF 358 NLMR++AC LFDA+Q CGAQANMAK+LAAKA+WEAANVCLQTHGGFGFACEYDVERKF Sbjct: 300 NLMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKF 357 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 173 bits (438), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 120/364 (32%), Positives = 190/364 (52%), Gaps = 28/364 (7%) Query: 7 SPDAYQDLRDAVRDLCSTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGL 66 SP+ ++ +RDA+R + D +P E + L + G + +P ++GG+G+ Sbjct: 4 SPE-HEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGM 62 Query: 67 GLTEASVIMEEINRTGG---------NSGACHGQMYNMGTLLRHGSAEQKQLYLPKIASG 117 + +EEI G NS AC G ++GS QK+ +L +A G Sbjct: 63 DYMSLVLALEEIAAGDGATSTIVSVQNSLAC-------GITEKYGSPTQKEQWLKPLARG 115 Query: 118 ELRLQSMGVTEPTTGTDTTKIKTTAVRKGDRYVVNGQKVWISRIQHSDLMILLARTTPLD 177 E+ L +TEP TG+D I+T A R GD +++NG K +I+ +H+ + I+ A T D Sbjct: 116 EM-LGCFCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVT---D 171 Query: 178 QVKKKSEGMSIFLVDLKHAIANGMSV-RPILNMVNH--ETNELFFDNLEIPVENLIGEEG 234 + K +G+S FLV G V R M H +T ++ DN +P L+G+EG Sbjct: 172 RSAGK-KGISCFLVPCD---TPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEG 227 Query: 235 KGFKYILDGLNAERTLIAAECIGDGYWFIEKAAQYAKERVVFDRPIGMNQGVQFPIADSY 294 +G++ L L A R IAA+ +G E A YA++R F PI +Q V F +AD Sbjct: 228 EGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMN 287 Query: 295 IELEAANLMRFKACELFDARQPCGAQANMAKFLAAKASWEAANVCLQTHGGFGFACEYDV 354 L+AA LM ++A +L D +PC +A+MAK A++A+ + A+ +Q HGG G+ ++ V Sbjct: 288 TLLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPV 347 Query: 355 ERKF 358 ER + Sbjct: 348 ERIY 351 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 161 bits (407), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 109/355 (30%), Positives = 183/355 (51%), Gaps = 21/355 (5%) Query: 14 LRDAVRDLCSTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGLGLTEASV 73 LR+AV + ++D + +P + L G L + EYGG+GLG + Sbjct: 18 LREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAHII 77 Query: 74 IMEEINRTGGNSGACHGQMYNM--GTLLRHGSAEQKQLYLPKIASGELRLQSMGVTEPTT 131 MEEI+R + G +G N+ + R+G+ EQK+ YLP++ SG+ + ++ ++EP Sbjct: 78 AMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDF-IGALAMSEPNA 136 Query: 132 GTDTTKIKTTAVRKGDRYVVNGQKVWISRIQHSDLMILLARTTPLDQVKKKSEGMSIFLV 191 G+D +K A +KGDRYV+NG K+WI+ +D++++ A+T ++ + GM+ FLV Sbjct: 137 GSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKT----DLEAGARGMTAFLV 192 Query: 192 DLKHAIANGMSVRPILN---MVNHETNELFFDNLEIPVENLIGEEGKGFKYILDGLNAER 248 + + G SV L+ M T EL F + E+P EN++G G+G ++ GL+ ER Sbjct: 193 EKGY---KGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFER 249 Query: 249 TLIAAECIGDGYWFIEKAAQYAKERVVFDRPIGMNQGVQFPIADSYIELEAANLMRF--- 305 ++++ +G ++ Y +R F + IG Q +Q +AD Y + A + Sbjct: 250 SVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVG 309 Query: 306 KACELFDARQPCGA----QANMAKFLAAKASWEAANVCLQTHGGFGFACEYDVER 356 +AC+ D+ A A + A KA+W A +Q+ GG G+ EY V R Sbjct: 310 QACDRADSADKVRALRKDAAGAILYSAEKATWMAGE-AIQSLGGNGYINEYPVGR 363 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 149 bits (375), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 106/348 (30%), Positives = 171/348 (49%), Gaps = 10/348 (2%) Query: 14 LRDAVRDLCSTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGLGLTEASV 73 + + RD + + K DE +P + + + G+ P GG GL +A+V Sbjct: 12 FQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATV 71 Query: 74 IMEEINRTGGNSGACHGQMYNMGTLL--RHGSAEQKQLYLPKIASGELRLQSMGVTEPTT 131 + EE+ R S A + ++NM + + G K + ++A+G ++ S +TEP + Sbjct: 72 VFEELARAC-TSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGR-KIGSYCLTEPGS 129 Query: 132 GTDTTKIKTTAVRKGDRYVVNGQKVWISRIQHSDLMILLARTTPLDQVKKKSEGMSIFLV 191 G+D +KTTA + YV+NG K +IS +D+++++ART + + G+S +V Sbjct: 130 GSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-----GEGARGVSAIVV 184 Query: 192 DLKHAIANGMSVRPILNMVNHETNELFFDNLEIPVENLIGEEGKGFKYILDGLNAERTLI 251 + + + T + FDN+++P ++L+G+EG+GF + + GL+ R I Sbjct: 185 PGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRINI 244 Query: 252 AAECIGDGYWFIEKAAQYAKERVVFDRPIGMNQGVQFPIADSYIELEAANLMRFKACELF 311 A +G ++ A Y KER F RPI Q +QF +AD EL AA M A Sbjct: 245 ATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAATKL 304 Query: 312 DARQP-CGAQANMAKFLAAKASWEAANVCLQTHGGFGFACEYDVERKF 358 DA P MAK LA + N LQ HGG+G+ EY +ER F Sbjct: 305 DAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYF 352 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 80/313 (25%), Positives = 144/313 (46%), Gaps = 24/313 (7%) Query: 16 DAVRDLCSTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGLGLTEASVIM 75 D +R + T P K DE F A+ AG++ AL+P +GG+ L + ++ Sbjct: 24 DVLRKVGPTITP--LVKPDERFYATRPFYQAMADAGFIKALLPESFGGTALSTLDFALAA 81 Query: 76 EEINRTGGN-SGACHGQMYNMGTLLRHGSAEQKQLYLPKIASGELRLQSMGVTEPTTGTD 134 EE+ N A G + ++ HG+ EQKQ +LP RL ++ TE T G + Sbjct: 82 EELTAVDVNVPSALLGTGLGLHAVVHHGTPEQKQRFLPDFIEDGSRLAALAFTEVTGGAN 141 Query: 135 ------TTKIKTTAVRKGDRYVVNGQKVWISRIQHSD-----LMILLARTTPLDQVKKKS 183 + ++T A +GD +V+NG K + + D L ++ RT P Sbjct: 142 YDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGKGAHLYSVICRTDP---ALPPQ 198 Query: 184 EGMSIFLVDLKHAIANGMSVRPILNMVNHETN---ELFFDNLEIPVENLIGEEGKGFKYI 240 + +++ +V A G+ + +L+ + H + F+N+ +P N++G+ G G + Sbjct: 199 QSLAVIMVP---GNAPGIEIVGLLDTMGHRATISPRVHFNNVRVPASNIVGKPGDGISML 255 Query: 241 LDGLNAERTLIAAECIGDGYWFIEKAAQYAK-ERVVFDRPIGMNQGVQFPIADSYIELEA 299 + LI + C+G + A ++A+ +R P+ +Q V F +AD + +EA Sbjct: 256 EAAFSWTAALIGSACVGVMRTAFDIALRFAQSDRRSGTIPVIEHQNVGFMLADIKMRIEA 315 Query: 300 ANLMRFKACELFD 312 A + +K+C D Sbjct: 316 ARYLTWKSCHQLD 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,122,482 Number of extensions: 45020 Number of successful extensions: 160 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 11 Length of query: 372 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 285 Effective length of database: 1,207,925 Effective search space: 344258625 Effective search space used: 344258625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)