BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0114.p (2215 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin prote... 157 1e-38 gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer... 133 1e-31 >gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin protein [Herbaspirillum seropedicae SmR1] Length = 2658 Score = 157 bits (397), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 214/676 (31%), Positives = 318/676 (47%), Gaps = 128/676 (18%) Query: 114 PVFAAPIADPAAPIRFQPQVTQSTGTGGGVPVVNITAPNAAGISLNQYSQFNVDAIGLIL 173 P + +ADP A +P V T G P+V I P+AAG+S NQYSQFNV G IL Sbjct: 35 PGYPQIVADPNAGAGKRPVVDN---TANGRPLVQIATPSAAGVSHNQYSQFNVGPNGAIL 91 Query: 174 NNSLITGGSTLGGTVSANTALGGRQASQIINQVTTQGSASTIAGTIEVFGRQADVIIANP 233 NN+ + GG V AN L G A I+N+VT+ S S + G EV G++A+VIIANP Sbjct: 92 NNARDVVLTQQGGYVGANPNLQGGAARVILNEVTST-SPSQLRGYTEVAGQRAEVIIANP 150 Query: 234 NGINCTACGFINTPRVVMTTGTPQFLSARGGAVSNFDDAKALSYDVRGGHIQIEGLAGAS 293 NGI+C CGFINT R V+TTG P F GG + ++ V G IQI G+ Sbjct: 151 NGISCDGCGFINTSRGVLTTGVPLF----GG------NGSLEAFRVSRGAIQI----GSG 196 Query: 294 GTPGAGIEGSAGVIDLIGGSIGVNASINAGQQINLISGQQTVSEAVAGQGKVGSDYNVAA 353 G GA + +DLI S+ VN + G ++NL++G V+ G +G +A Sbjct: 197 GLNGANTD----QLDLIARSVQVNGEL-WGNRLNLVTGANRVN-----YGDLGLSV-IAG 245 Query: 354 NGSTPNFGGPNPALQIDATAFGAMNAGQIKVIGTTAGLGVRA-GVLNANNGNVTIDANGD 412 G+ P G +D G M A +I ++GT G+GVR+ G L A G+ T+ + G Sbjct: 246 EGNAPTIG-------VDVARMGGMYAQKIMLVGTENGVGVRSLGTLAAQAGSFTLSSQGQ 298 Query: 413 VNATSVYGSQGASIRANGGVSVGSVTSYGATSVTAGTDVAIGQMTAANNTQI--SGRNVA 470 + + G TS A D+ + +N + +GRN Sbjct: 299 IALS------------------------GKTS--ASDDIVLKSAVGTSNDGVLYAGRNAQ 332 Query: 471 LGNTVV--GGALAINGSGNVTTSQ---TGAIVGDVQVSAGGMFYNSGNWTTGGNLTITAN 525 L V+ G L+ G+ ++T +Q TG + V +GN GNLT+TA+ Sbjct: 333 LQGGVIENAGTLSAQGNLDITAAQLRSTGTLAAGVD--------QAGNVAQAGNLTVTAS 384 Query: 526 TVGNALSSQLAGLASTSVTANNLANYGTLYGDNLRINVSGALDNSFGSMLAKSLLVANVN 585 G+ L+ T N A G +L++ V G +D S S A L A Sbjct: 385 --GDVLA-----------TGVNRA------GADLQL-VGGKIDLSHSSSSAGRKLGAQA- 423 Query: 586 SLASNLGGTIYVGDPNAAANATPSGDLALTVSGAGSSFNNLGGLIAASNDLTVSAANTAL 645 S A LGG A T +GDL L G++ G + +S D ++A Sbjct: 424 SDALTLGG---------ATVETLAGDLEL----GGATITLDGAKLTSSADARINAVAGL- 469 Query: 646 DLSSTTSGQLNAGGALTLNAGVLVNSGAWMASGSRVSLSGVNGVVNAGVIQSSGDLSLSS 705 S G++ + GA+ + A + NS +++ + +GV + +AG I S+G +++ Sbjct: 470 ---SNRGGEITSAGAMNVTAASVDNSAGMISAAT--GGAGVLSLRSAGSI-SNGQGRIAA 523 Query: 706 SAGV------ITNAGALVAGRDLTAS-GTMNNLAGAVLHAERDL--TLSGTGTNAGTIEA 756 A + I G+++A D T S ++NN AG+++ + RDL T SG N G ++A Sbjct: 524 GAALNLAAASIQGQGSVIANGDATLSLQSLNNEAGSLIQSNRDLRITTSGATGNQGALKA 583 Query: 757 LRNVVLQGSNFDNSTG 772 + + L S DN+ G Sbjct: 584 IGALNLTASQIDNAAG 599 >gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 2685 Score = 133 bits (335), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 182/633 (28%), Positives = 279/633 (44%), Gaps = 149/633 (23%) Query: 116 FAAPIADPAAPIRFQPQVTQSTGTGGGVPVVNITAPNAAGISLNQYSQFNVDAIGLILNN 175 +A IADP AP QP V T G+P VNI P+AAG+S N YSQF+V+ G ILNN Sbjct: 71 YAQIIADPNAPRAQQPVVVN---TANGIPQVNIQTPSAAGVSRNTYSQFDVNRAGAILNN 127 Query: 176 SLITGGSTLGGTVSANTALGGRQASQIINQVTTQGSASTIAGTIEVFGRQADVIIANPNG 235 S + LGG V N L A I+N+V + + S + G +EV G +A VIIANP+G Sbjct: 128 SRSDVQTQLGGWVQRNPNLANGTARVILNEVNSS-NPSMLRGFVEVAGDRAQVIIANPSG 186 Query: 236 INCTACGFINTPRVVMTTGTPQFLSARGGAVSNFDDAKALSYDVRGGHIQIEGLAGASGT 295 + C CGFIN R +TTGTP ++ Y VRGG + I+ Sbjct: 187 VTCDGCGFINASRSTLTTGTPI-----------INNGSLDGYLVRGGVVSIK-------- 227 Query: 296 PGAGIEGS-AGVIDLIGGSIGVNASINAGQQINLISGQQTVSEAVAGQGKVGSDYN---- 350 G G+ S + DLI + +NA I + + + G +V +D Sbjct: 228 -GNGLNDSQSSFTDLIARAAKINAGIWSKEL-----------KVTVGTNQVNADNTTVTA 275 Query: 351 VAANGSTPNFGGPNPALQIDATAFGAMNAGQIKVIGTTAGLGVR---------------- 394 V+A+ + P FG ID G M A +I ++GT +G+GVR Sbjct: 276 VSADPAKPQFG-------IDVAQLGGMYANKIVLVGTESGVGVRNAGAIGAAAGELTLTA 328 Query: 395 AGVLNANNGNVTIDANGDVNATSVYGS-----------QGASIRANGGVSVGSVTSYGAT 443 AG + N+G++ A+ V AT G ++A+GG++ + Sbjct: 329 AGRIE-NSGSLAATASLGVRATGQGGDIVNSGSVASSAAAVKLQADGGIN-------NSN 380 Query: 444 SVTAGTDVAIGQMTAANNTQISGRNVALGNTVVGGALAINGSGNVTTSQTGAIVGDVQVS 503 +++A + V + + N+ IS A GN V A+N SG V + GD+ +S Sbjct: 381 TISAQSGVTLTAASLQNSGAIS---AATGNVEVDTHGAVNNSGKVQAA------GDISIS 431 Query: 504 -------AGGMFYNSGNWTTGGNLTITANTVGNALSSQLAGLASTSVTANNLANYGTLYG 556 A N+GN G L +TA+ + L+N G++ Sbjct: 432 ATQPQGNAAADISNTGNIEAAGQLGLTADGM--------------------LSNDGSITA 471 Query: 557 DNLRINVSGALDNSFGSMLAKSLLVANVNSLASNLGGTIYVGDPNAA-ANATPSGDLALT 615 ++R V G +N GS+ + SN T+ G A + DL LT Sbjct: 472 QSIRAGVQGLRNN--GSIGTR-----------SNAAFTLQQGLVQGANGRLSAVSDLNLT 518 Query: 616 VSGAGSSFNNLGGLIAASNDLTVSAAN---------TALDLSSTTSGQLNAGGALTLNAG 666 + G ++N G + A N LT+SAAN A D++ T +L+ G ++ N Sbjct: 519 MQG---DYDNAGRVQA--NTLTLSAANIGNRSGAALQAADITLTALNRLDNAGEISANRQ 573 Query: 667 VLVNSGAWMASGSRVSLSGVNGVVNAGVIQSSG 699 + + + + + S ++ G + VVNA ++++G Sbjct: 574 LNITADSILNS---ATILGKDLVVNARTLENTG 603 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.128 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 6,002,434 Number of extensions: 246061 Number of successful extensions: 971 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 847 Number of HSP's successfully gapped: 20 Length of query: 2215 Length of database: 1,619,870 Length adjustment: 101 Effective length of query: 2114 Effective length of database: 1,141,635 Effective search space: 2413416390 Effective search space used: 2413416390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 138 (57.8 bits)