BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0112.p (596 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ... 152 9e-38 gi|300310625|ref|YP_003774717.1| hemolysin activation translocat... 123 5e-29 gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ... 56 1e-08 >gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 584 Score = 152 bits (383), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 151/549 (27%), Positives = 240/549 (43%), Gaps = 77/549 (14%) Query: 85 ADVEQLP-ESGPTFLIHQIDIAG------NTVLSESELNQIIQPFLNKSLGGARINLLLR 137 A E +P + P F +H++ + G + +L E N + LG IN ++ Sbjct: 76 AAAEAVPAQESPCFAVHRVVLDGPGSARFDWLLDLPEANPG-----GRCLGVQGINAVMS 130 Query: 138 RFTEAFVKNGLITTRAYLGEQNLKSGVLVITFVPGKVESIQLNGQALSPRKDGATPDSGG 197 A V G +TTR Q++ SG L +T VPGK+ +I+ D A P + Sbjct: 131 ALQNAIVARGYVTTRILAQPQDIASGELRLTIVPGKIRAIRF--------ADNADPRATQ 182 Query: 198 WLTELGVRMAVPTGPGDLLNLTDLEQGVEQINRMRRNKAEMQILPG----TTPGGSIIGL 253 W A+P PGDLLNL D+EQG+E + R+ +A++QI P PG S + + Sbjct: 183 W-------NALPASPGDLLNLRDIEQGLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVI 235 Query: 254 NNKVGDRLWFNAGIDNYGSQQTGLNRTRIGVEADNLLGFQEILSFNYS--------GSRE 305 + + G + +D+ G++ TG + V DN L ++ ++S G+R Sbjct: 236 SYQQGIPIRLTLSVDDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHDLGGGDPGARG 295 Query: 306 TNALVASASVPFGYNTFSYTASLSEYQTL---IGDTALLYGNTVANTWAWNRVIARSQTA 362 + + V S+P GY ++T S + Y I T L G + N +R++ R Sbjct: 296 SQSQVVHYSLPLGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLSRLVYRDAVR 355 Query: 363 KTAVDTSLSLRRADRDINNLTLDPQKLTVLRVGINTLQRFLVRQQPAALTLEAGISKGLS 422 KT + +R + +++ + Q+ V + R + + L L +G Sbjct: 356 KTTLSFKGFVRSSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGE--GTLDLALAYKRGTG 413 Query: 423 AFGANKDDAQIQQNEAHSQFTK------VDFNGSITAALP---NVASMALTLKSQVNGQW 473 AF DA EA + T D N + ALP +M TL ++ QW Sbjct: 414 AF-----DAMAAPEEAFGEGTSRFRLMTADLNLNAPFALPMPWGKQAMRYTLGARA--QW 466 Query: 474 SRQSLFGSEQLFVGGMSSVRGFR-ENGISGDRGAYARNEVIWANAPSLSDVRFEPYIFFD 532 +R L ++ +GG SVRGF E+ + +RG Y RNE+ A +L + E Y D Sbjct: 467 NRTPLTPQDKFSLGGRYSVRGFDGESNLLAERGWYVRNEL----AAALGASKQEIYAGLD 522 Query: 533 MG-----LTELISEQRTRKLSGAGVGVRMQLQLEKQGWSSEITLGRPIQQPDYLEKRSVL 587 G TEL+ R+L+GA +G+R L E+ G PI QPD + + Sbjct: 523 YGEVGGPSTELLV---GRRLAGAVLGLRGALGRTNY----EVFAGVPISQPDRFKTSPLT 575 Query: 588 LLATLNWSY 596 +L WS+ Sbjct: 576 AGMSLMWSF 584 >gi|300310625|ref|YP_003774717.1| hemolysin activation translocator protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 123 bits (308), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 110/416 (26%), Positives = 184/416 (44%), Gaps = 44/416 (10%) Query: 117 QIIQPFL----NKSLGGARINLLLRRFTEAFVKNGLITTRAYLGEQNLKSGVLVITFVPG 172 IQ +L LG I +LRR T ++ G TTR +G+Q+L +G L IT VPG Sbjct: 112 HFIQTYLAGYAGSCLGQQGIETILRRVTGQILERGYSTTRVGIGQQDLSTGQLSITLVPG 171 Query: 173 KVESIQLNGQALSPRKDGATPDSGGWLTELGVRMAVPTGPGDLLNLTDLEQGVEQINRMR 232 + +I+ A++ R A PT G LLNL DLEQG+EQ+ R+ Sbjct: 172 IIHAIRFKDPAITA----------------SYRSAFPTQAGALLNLRDLEQGLEQMKRVA 215 Query: 233 RNKAEMQILPGTTPGGSIIGLNNKVGDRLWFNAGIDNYGSQQTGLNRTRIGVEADNLLGF 292 +MQILP PG S + L K +D+ G++ TG + I + D+LLG Sbjct: 216 SQDVDMQILPTDVPGQSDVVLEVKRIKPWKLTLSLDDSGAKSTGKYQAGINLAIDDLLGI 275 Query: 293 QEILSFNYSG-------SRETNALVASASVPFGYNTFSYTASLSEYQTLIGDTALLYGNT 345 ++ + +S +R T S S P GY TF + S S Y I A L+ Sbjct: 276 NDLFNAGFSSDADRMGQARGTTGNNFSYSAPAGYWTFGVSGSTSRYHQRI---AGLFQTF 332 Query: 346 VANTWAWN------RVIARSQTAKTAVDTSLSLRRADRDINNLTLDPQKLTVLRVGINTL 399 V++ + N ++ R K ++ + R + I++ ++ Q+ + + Sbjct: 333 VSSGHSENLEVRVQKLFQRDAQQKNSLQLRIGKRWSRSYIDDTEIEVQRRNTTFAELGWM 392 Query: 400 QRFLVRQQPAALTLEAGISK--GLSAFGANKDDAQIQQNEAHSQFTKVDFNGSITAALPN 457 R + L+A ++ G+S F D + +++ + ++ A Sbjct: 393 HRHYF----GDVQLDATLANRWGVSWFNGAADAPDRLSDGPTYRYSMQTIDATVVAPF-K 447 Query: 458 VASMALTLKSQVNGQWSRQSLFGSEQLFVGGMSSVRGFR-ENGISGDRGAYARNEV 512 + +LT + GQ SR L+ ++Q +G +VRGF E ++ + G + RN++ Sbjct: 448 LGGQSLTYVGTLRGQHSRSRLYLTDQFAIGSRYTVRGFDGELTLASESGVFLRNDL 503 >gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 569 Score = 55.8 bits (133), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 110/499 (22%), Positives = 190/499 (38%), Gaps = 56/499 (11%) Query: 97 FLIHQIDIAGNTVLSESELNQIIQPFLNKSLGGARINLLLRRFTEAFVKNGLITTRAYLG 156 F I + +AGNT+L + ++ + PF + I L + G + ++ Sbjct: 53 FEIRRFVVAGNTLLPQDQVQAAVAPFSGQGRVYGDIQQALEALENLYRSAGYTAVQVHVP 112 Query: 157 EQNLKSGVLVITFVPGKVESIQLNGQALSPRKDGATPDSGGWLTELGVRMAVPT-GPGDL 215 EQ L SG + I V V +Q++ ++ +R ++P G Sbjct: 113 EQELSSGEVRIDVVETVVGKVQVS--------------ENRHFSQANIRASIPALKEGVP 158 Query: 216 LNLTDLEQGVEQINRMRRNKAEMQILPGTTPGGSIIGLNNKVGDRLWFNAGIDNYGSQQT 275 NL + + V+ N + + + + + K D L +DN GS T Sbjct: 159 PNLRRISESVQLANDNPAKQVNVTLSAAQSGDQIDAKIEVKDDDPLRVMLNLDNTGSADT 218 Query: 276 GLNRTRIGVEADNLLGFQEILSFNYSGSRETNALVASASVPFGYN----------TFSY- 324 G RT + ++ NL + + Y+ S ++ + GY F Y Sbjct: 219 GRWRTGVAIQHSNLFNRDHVATVAYTTSPDSPSGSKVDLYSLGYRLPLYELGDSLDFIYG 278 Query: 325 TASLSEYQTLIGDTAL-LYGNTVANTWAWNRVIARSQTAKTAVDTSLSLRRADRD--INN 381 +S+S QTL ++ L + G WN AR+ + + ++ D IN Sbjct: 279 KSSVSSGQTLALNSTLNITGRGEVYALRWNHFFARNGEWSSKLVLGADYKKVDSSCAING 338 Query: 382 LTLDPQKL-TVLRVGINTLQ-RFLVRQQPAALTLEA--GISKGLSAFGANKDDAQIQQNE 437 L+ L T L L + ++Q ++A G+++ ++ A ++DA +Q + Sbjct: 339 GLLNGATLNTCLPYSTLPLSLTYSAQRQGVGEVIDANIGVARNMATGQARRNDA-LQVTD 397 Query: 438 AHSQFTK----------VDFNGSITAALPNVASMALTLKSQVNGQWSRQSLFGSEQLFVG 487 +S FT V S A N M L N Q S +L EQ + Sbjct: 398 RYSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQMRLA----GNLQLSPHALPAVEQFGLA 453 Query: 488 GMSSVRGFRENGISGDRGAYARNEVIWANAPSLSDVR--FEPYIFFDMG------LTELI 539 G ++VRGF E +S D G +E+ + + S+++ F D G +T Sbjct: 454 GATAVRGFTERAVSADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDAGRGANNNVTANS 513 Query: 540 SEQRTRKLSGAGVGVRMQL 558 Q T L+ G G+R L Sbjct: 514 QVQSTLTLASIGAGLRYAL 532 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,820,133 Number of extensions: 76792 Number of successful extensions: 249 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 237 Number of HSP's successfully gapped: 4 Length of query: 596 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 505 Effective length of database: 1,188,985 Effective search space: 600437425 Effective search space used: 600437425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)