BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0110.p (771 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase... 1147 0.0 gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 727 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 124 2e-29 gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 110 3e-25 gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family ... 63 9e-11 >gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 790 Score = 1147 bits (2966), Expect = 0.0, Method: Compositional matrix adjust. Identities = 540/771 (70%), Positives = 636/771 (82%), Gaps = 3/771 (0%) Query: 1 MQKEDDPRKAHFKKYQNAAGGWGSVRALSSILGRERVLVKGTKVLLQQNKPDGFACVSCS 60 M+ +D P A F +Y NAAGGWGSV+AL SIL +E V+ GT++L+ QNKP+GFACVSCS Sbjct: 18 MKTKDIPEDATFDEYNNAAGGWGSVKALGSILQQEHVIASGTRILMHQNKPEGFACVSCS 77 Query: 61 WAKPADPHAFEFCENGAKATAWEITSKRADASLFERHTVRELEGWSDMELEAAGRLTTPL 120 WAKPADPH FEFCENGAKATAWEIT KRA A+ F +HTV E+E W+D+ELE++GR+T P+ Sbjct: 78 WAKPADPHLFEFCENGAKATAWEITRKRATAAFFAQHTVTEMEAWNDLELESSGRVTEPM 137 Query: 121 RYEASSDKYVPVAWDDAFDAIAADLRAMEPDGVVFYSSGRASLEASYLYQLMARMYGNNH 180 +Y+A+SD+Y+PV+W +AFD IAA LRAM+P VVFY+SGRASLE SYLYQLMARMYG N+ Sbjct: 138 KYDAASDRYLPVSWSEAFDDIAAQLRAMDPKKVVFYASGRASLETSYLYQLMARMYGTNN 197 Query: 181 LPDSSNMCHESTSVALPKSIGVPVGTLTLEDFQHTDCILFFGHNTGTNAPRMLHQLEEVR 240 LPDSSNMCHESTSVALP++IGVPVGT+TL+DF TDCILFFGHNTGTNAPRMLH LEEVR Sbjct: 198 LPDSSNMCHESTSVALPQTIGVPVGTVTLDDFGQTDCILFFGHNTGTNAPRMLHPLEEVR 257 Query: 241 KRGVPIVTFNPIRERGLEKFVNPQSPLQMATPAHTDVSTQYVRVKVGGDIAALTGMMKWI 300 KRGVP++TFNP+RERGLE+FVNPQSP +M TPAHTD+STQY+++K+GGD AA GM K I Sbjct: 258 KRGVPVITFNPLRERGLERFVNPQSPKEMVTPAHTDISTQYLQIKIGGDSAAAIGMAKRI 317 Query: 301 LETDDEKRMNGGERIVDVNFIAEHSQGFEELAAAIRKVNWTDIEKASGVSREELIQAART 360 LE DD+ R G R++D+ FI EH+ GFE+ A A+R W ++E+ +GVSR EL AA T Sbjct: 318 LERDDKARQEGLPRLLDLAFIEEHTSGFEQFADAVRAAPWDELERHAGVSRAELELAADT 377 Query: 361 YVNAKAVMLMYGMGVTQHREGVQTIHMLTNLLLLRGNIGKPGAGICPIRGHSNVQGQRTV 420 Y A MLMYGMGVTQHRE V+TIHMLTNLLL+RGNIGKPGAGICPIRGHSNVQGQRTV Sbjct: 378 YAGAGRAMLMYGMGVTQHREAVRTIHMLTNLLLMRGNIGKPGAGICPIRGHSNVQGQRTV 437 Query: 421 GITEKPELVPMDKLKEQYGFEPPSKKGWNTVEACEAVLAKNVSAFFMLGGNFVRAVPDHR 480 GITEKPELVP DKL+ Y FEPP +KG NTVEACE + +SAFFMLGGNFVRA+PDH Sbjct: 438 GITEKPELVPNDKLRALYHFEPPMEKGLNTVEACEKIRDGELSAFFMLGGNFVRAIPDHG 497 Query: 481 RIEPAWREIPLTVQVLTKLNRSALIHGKKSYLLPCLGRIEIDRQASGPQAVSMEDSTGCM 540 +E AWR++PLTVQV+T NRS +IHG+ SY+LPCLGRIEIDRQ G QAVS+EDSTGCM Sbjct: 498 VMEAAWRKLPLTVQVVTHFNRSCVIHGQTSYVLPCLGRIEIDRQRGGEQAVSVEDSTGCM 557 Query: 541 HGSRGQAEPASPELLSEVAIIGQLAKRLLPFNPKVTWQTWMDDYSTIRDAIEITYPKIFK 600 HGSRG+AEPASP LLSE AI+ +LAKRLLPFNP+V W W+ DY+ IRDAIE TYP IF+ Sbjct: 558 HGSRGRAEPASPHLLSEPAIVAELAKRLLPFNPQVDWDGWVGDYARIRDAIEATYPDIFR 617 Query: 601 DFNRRMWNPGGFARPMGARTRQWETPNGKANFFPPTCLDADPDMPPPSGDVLRLMTTRGD 660 DFN RMWNPGGFARP+ AR RQW+TPNG+ANF P D DPDMP + +VLRLMTTRGD Sbjct: 618 DFNARMWNPGGFARPLAARERQWKTPNGRANFMTPDGFDPDPDMPGDAPEVLRLMTTRGD 677 Query: 661 SQFNTTVYGLSDRFRGVHGTRKVLLMHRADIQQLQLSEGDIVKAISVVPPGEDHIDRSVG 720 SQFNTTVY L DRFRGV GTR+VLLM+ D+QQ L+ GD V A +V D ++R V Sbjct: 678 SQFNTTVYSLDDRFRGVWGTRRVLLMNLRDLQQHGLAAGDFVCAETV---SADGVERRVP 734 Query: 721 GLRIQAFDIPRGCVLGYYPELNPLIPLSHYAKESKVPAVKSVPIRLLRDDR 771 LR++ FDIP GCV+GY+PELN LIPL H+A+ SKVPA KSVPIRL+ + R Sbjct: 735 HLRVEPFDIPVGCVMGYFPELNRLIPLFHHARGSKVPAAKSVPIRLIPELR 785 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 727 bits (1876), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/773 (47%), Positives = 490/773 (63%), Gaps = 18/773 (2%) Query: 7 PRKAHFKKYQNAAGGWGSVRALSSILGRERVLV-KGTKVLLQQNKPDGFACVSCSWAKPA 65 P K ++Y AGGWG+++ ++ L +E V +G + LL QN+PDGF C C+W Sbjct: 2 PSKPRIEQYNQPAGGWGALKYVALNLVKEHVADGRGLRTLLSQNQPDGFDCPGCAWPDRE 61 Query: 66 DPHAFEFCENGAKATAWEITSKRADASLFERHTVRELEGWSDMELEAAGRLTTPLRYEAS 125 FEFCENG KA A E T KR F +HTV EL SD ELE GRLT P+ Y+A+ Sbjct: 62 HTSTFEFCENGVKAVAAEATKKRVTPEFFAQHTVTELMQQSDFELEEHGRLTHPMVYDAA 121 Query: 126 SDKYVPVAWDDAFDAIAADLRAM-EPDGVVFYSSGRASLEASYLYQLMARMYGNNHLPDS 184 +DKY + W DAF +A L A+ +PD FY SGRAS EA++L+QL R YG N+ PD Sbjct: 122 TDKYKKIEWADAFALMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPDC 181 Query: 185 SNMCHESTSVALPKSIGVPVGTLTLEDFQHTDCILFFGHNTGTNAPRMLHQLEEVRKRGV 244 SNMCHE TSV LP ++G+ GT+ LEDF H D +L FG N TN PRM+ +L KRG Sbjct: 182 SNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRGA 241 Query: 245 PIVTFNPIRERGLEKFVNPQSPLQMATPAHTDVSTQYVRVKVGGDIAALTGMMKWILETD 304 IV NP++ERGLE+F +PQS ++M T T +S+ ++ +GGD+A + G++K +E D Sbjct: 242 TIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIELD 301 Query: 305 DEKRMNGGERIVDVNFIAEHSQGFEELAAAIRKVNWTDIEKASGVSREELIQAARTYVNA 364 D R G ER++DV FI EH+ GFEE AA R +W DI SGVSRE++ + A+ YV Sbjct: 302 DAARAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDIVAESGVSREDIEKLAQVYVKG 361 Query: 365 KAVMLMYGMGVTQHREGVQTIHMLTNLLLLRGNIGKPGAGICPIRGHSNVQGQRTVGITE 424 KAV+ +GMG+TQH+ V TI +L+N+++LRGNIG+ GAG+CP+RGHSNVQG RTVGI E Sbjct: 362 KAVIATWGMGLTQHKHAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGIDE 421 Query: 425 KPELVPMDKLKEQYGFEPPSKKGWNTVEACEAVLAKNVSAFFMLGGNFVRAVPDHRRIEP 484 KP +D+L++ +GF+ P G +TV + A+L F LGGNF A PD R Sbjct: 422 KPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTFE 481 Query: 485 AWREIPLTVQVLTKLNRSALIHGKKSYLLPCLGRIEIDRQASGPQAVSMEDSTGCMHGSR 544 A R LTV + TKLNRS L+HGK + +LP +GR EID Q SGPQ V++EDS +H S Sbjct: 482 AMRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDMQKSGPQGVTVEDSMSMVHVSY 541 Query: 545 GQAEPASPELLSEVAIIGQLAKRLLPF---NPKVTWQTWMDDYSTIRDAIEITYPKIFKD 601 G +PAS L+SE AI+ LA+ + PK+ W + +DYS IRDAIE Y FK Sbjct: 542 GINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEQVY-DAFKG 600 Query: 602 FNRRMWNPGGFARPMGARTRQWETPNGKANFFPPTCLDADPDMPPPSG------DVLRLM 655 +N R+ NPGGF + +R R W+T +GKANF D D P +L LM Sbjct: 601 YNERIANPGGFHLGVASRDRVWKTASGKANFI---VHDIPKDTPIHRARAIHGEKLLTLM 657 Query: 656 TTRGDSQFNTTVYGLSDRFRGVHGTRKVLLMHRADIQQLQLSEGDIVKAISVVPPGEDHI 715 TTR Q+NTT+YG+ DR+RGV G R+V+ ++ AD+Q L L +G+ V SV +D + Sbjct: 658 TTRSHDQYNTTIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGEWVDITSV---WDDGV 714 Query: 716 DRSVGGLRIQAFDIPRGCVLGYYPELNPLIPLSHYAKESKVPAVKSVPIRLLR 768 +R G + ++IPRGC+ YYPE NPL+PL +A +++ P KS+P+ L R Sbjct: 715 ERRAEGFLLVEYEIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLSR 767 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 124 bits (312), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 98/408 (24%), Positives = 185/408 (45%), Gaps = 45/408 (11%) Query: 115 RLTTPLRYEASSDKYVPVAWDDAFDAIAADLRAMEP----DGVVFYSSGRASLEASYLYQ 170 R+ P+ ++ +D + V+W++A A++ R ++ D + +S R + E +YL Q Sbjct: 298 RILNPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQ 357 Query: 171 LMARM-YGNNHLPDSSNMCHESTSVALPKSIGVPVGTLTLEDFQHTDCILFFGHNTGTNA 229 + R +GNN++ + +CH T L +++G GT T E +D IL G N Sbjct: 358 KLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGH 417 Query: 230 PRMLHQLEEVRKRGVPIVTFNPIRERGLEKFVNPQSPLQMATPAHTDVSTQYVRVKVGGD 289 P Q++ ++G ++ +P R E +P +Q + +++++ G + Sbjct: 418 PVFASQIKRRVRQGAKLIVIDP---RTTEMVKSPH--IQ---------ADYHLKLRPGTN 463 Query: 290 IAALTGMMKWILETDDEKRMNGGERIVDVNFIAEHS--QGFEELAA-AIRKVNWTDIEKA 346 A +T + IL E + D +I E Q +++ +R+ N + A Sbjct: 464 TAIITALAHVILS----------EGLQDEAYIQERCDLQSYQDWKEFVLREDNSPEAMAA 513 Query: 347 -SGVSREELIQAARTYVNAKAVMLMYGMGVTQHREGVQTIHMLTNLLLLRGNIGKPGAGI 405 +GV E + AAR Y + YG+GVT+H +G T+ + NL + GN+G+ G G+ Sbjct: 514 ITGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGREGVGV 573 Query: 406 CPIRGHSNVQGQRTVGITEKPELVP----------MDKLKEQYGFEPPSKKGWNTVEACE 455 P+RG +NVQG +G P +P ++ ++ +G + G + Sbjct: 574 NPLRGQNNVQGSCDMG--SFPHELPGYRHISDSTVRNQFEQAWGVTLNPEPGLRIPNMFD 631 Query: 456 AVLAKNVSAFFMLGGNFVRAVPDHRRIEPAWREIPLTVQVLTKLNRSA 503 A L + + G + V++ P+ + + A + + V LN +A Sbjct: 632 AALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECIVVQDLFLNETA 679 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 110 bits (276), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 101/430 (23%), Positives = 181/430 (42%), Gaps = 55/430 (12%) Query: 114 GRLTTPLRYEASSDKYVPVAWDDAFDAIAADLRAM----EPDGVVFYSSGRASLEASYLY 169 GRL P +A + +AWD A D +A+ A+ PD V Y SG+ E Y+ Sbjct: 68 GRLLHPQLRDAQG-QLQQLAWDQALDHVASKFSAIIAEHGPDAVALYVSGQLLTEDYYIA 126 Query: 170 -QLMARMYGNNHLPDSSNMCHESTSVALPKSIGVPVGTLTLEDFQHTDCILFFGHNTGTN 228 +LM G+ ++ +S +C S ++ G + + ED + D ++ G N Sbjct: 127 NKLMKGYIGSANIDTNSRLCMSSAVAGHKRAFGEDLVPVCYEDLELADLVVLVGSNAAWC 186 Query: 229 APRMLHQLEEVRKR--GVPIVTFNPIRERGLEKFVNPQSPLQMATPAHTDVSTQYVRVKV 286 P + ++ +++ + +V +P R A +++ ++ +K Sbjct: 187 HPILFQRIARLKETRPALRLVVIDPRRT------------------ATCELADLHLPIKP 228 Query: 287 GGDIAALTGMMKWILETDDEKRMNGGERIVDVNFIAEHSQGFEELAAAIRKVNWTD---I 343 G D+ G+M ++ + D FI H++GF+E A A+ + D + Sbjct: 229 GTDVWLFNGLMSYLAH----------RGLRDNAFIEAHTKGFDE-ALAVADADCADPATV 277 Query: 344 EKASGVSREELIQAARTYVNAKAVMLMYGMGVTQHREGVQTIHMLTNLLLLRGNIGKPGA 403 + + ++L+ + N + V+ + MGV Q G ++ + N L G IGKPG Sbjct: 278 ARLCKIDLKDLLAFYEAFANTEKVVTGFSMGVNQSSAGTDKVNSIINCHLATGRIGKPGM 337 Query: 404 GICPIRGHSNVQGQRTVGITEKPELVPMD-------KLKEQYGFEP--PSKKGWNTVEAC 454 G I G N G R VG MD ++ + + P K G V+ Sbjct: 338 GPFSITGQPNAMGGREVGGLANMLAAHMDLDQPLHRQIVQDFWQSPRIADKPGLKAVDMF 397 Query: 455 EAVLAKNVSAFFMLGGNFVRAVPDHRRIEPAWREIPLTVQ--VLTKLNRSALIHGKKSYL 512 A+ + A +++ N + ++PD ++ A ++ L V V+T+ + +AL H L Sbjct: 398 RAIEEGRIKAVWIMATNPMVSLPDADLVQRALQKCELVVSSDVITQTDTNALAH----VL 453 Query: 513 LPCLGRIEID 522 LP L E D Sbjct: 454 LPALAWGEKD 463 >gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 62.8 bits (151), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 71/309 (22%), Positives = 120/309 (38%), Gaps = 56/309 (18%) Query: 115 RLTTPLRY--EASSDKYVPVAWDDAFDAIAADLRAM---EPDGVVFYSSGRA-------S 162 RL P+R + K+ ++WD+A IA L + +P ++ YS S Sbjct: 61 RLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALRDPQAILPYSYAGTMGQVQGES 120 Query: 163 LEASYLYQLMARMYGNNHLPDSSNMCHESTSVALPKSIGVPVGTLTLEDFQHTDCILFFG 222 + + ++L G + L +C + +IG VG LE H IL +G Sbjct: 121 MAGRFFHRL-----GASQL--ERTICASAGGAGYRYTIGARVGA-DLEQVDHAKLILIWG 172 Query: 223 HNTGTNAPRMLHQLEEVRKRGVPIVTFNPIRERGLEKFVNPQSPLQMATPAHTDVSTQYV 282 N + +++ ++ G ++ +P R EK Q++ Sbjct: 173 GNPIASNLHFWTRVQTAKRNGARLIAIDPYRSLTAEK------------------CHQHI 214 Query: 283 RVKVGGDIAALTGMMKWILETDDEKRMNGGERIVDVNFIAEHSQGFEELAAAIRKVNWTD 342 + G D A GMM ++ D ++D ++IA H+ GFE+L R WT Sbjct: 215 ALLPGTDAALALGMMHVLISED----------LLDHDYIARHTLGFEQLRE--RVAEWTP 262 Query: 343 IEKA--SGVSREELIQAARTY----VNAKAVMLMYGMGVTQHREGVQTIHMLTNLLLLRG 396 A G+S EE++ AR Y + + V + G+ + R G + L L G Sbjct: 263 ARTAEVCGISAEEVLNLAREYGRVALAGEGVFIRANYGLQRVRGGGMAARNVACLPALVG 322 Query: 397 NIGKPGAGI 405 P G+ Sbjct: 323 AWRHPAGGV 331 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,517,070 Number of extensions: 108153 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 5 Length of query: 771 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 678 Effective length of database: 1,179,515 Effective search space: 799711170 Effective search space used: 799711170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)