BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0109.p (229 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311586|ref|YP_003775678.1| ATP-dependent DNA ligase [Herba... 201 4e-53 >gi|300311586|ref|YP_003775678.1| ATP-dependent DNA ligase [Herbaspirillum seropedicae SmR1] Length = 856 Score = 201 bits (511), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 104/228 (45%), Positives = 143/228 (62%), Gaps = 11/228 (4%) Query: 5 RALPQAID----PD---HPPYLEVAEPLGLLSAAQMNVLEFHTWNGTRTSPTKPDRFIFD 57 + +P+AID P+ H Y+ V + GL++ QM VLE H W T + PDR +FD Sbjct: 624 QGMPEAIDTIEVPEGEGHAQYMMVNDVAGLVATVQMGVLELHAWGATGAALNHPDRVVFD 683 Query: 58 LDPGSNVSWAAVQEAATLVRGFLQELGLQSLLKTSGGKGLHILVPLRRQHGWDTVKDFSH 117 LDP +V WA V E A LVRG L+E+GL LKTSGGKGLH++VP+R +H W VKD++H Sbjct: 684 LDPADDVPWATVVEGAQLVRGLLKEIGLDCFLKTSGGKGLHVMVPIRPEHDWAVVKDWAH 743 Query: 118 AIATHLAQTLPERFSSKSGSRNRVGRIFLDYIRNGFGATTACAWSLRARPGMGVSVPVSW 177 A HLA+ LP+RF +K R G+IF+DY+RN GATT A+S+RAR G VSVP++W Sbjct: 744 RFAAHLARHLPDRFIAKMSKAARNGKIFIDYLRNAPGATTVVAYSVRARDGAPVSVPITW 803 Query: 178 DELERLQ--SGSQWNISNIHTRLD--RGNAPWSNNSFINQSLTSAMRA 221 +ELE+ + + +S + R+ + PW++ Q LT AM A Sbjct: 804 EELEQGKGIKADHFRVSTMPARIRSLEQHDPWAHFGQAPQRLTKAMLA 851 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 813,583 Number of extensions: 34579 Number of successful extensions: 78 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 1 Length of query: 229 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 147 Effective length of database: 1,231,600 Effective search space: 181045200 Effective search space used: 181045200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)