BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0099.p (156 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 61 5e-11 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 60.8 bits (146), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 41/140 (29%), Positives = 72/140 (51%), Gaps = 9/140 (6%) Query: 1 MEALAEAKAQGLTRLIGVSNFTIAHLQQAIAVVGAQEIATQQIEIHPFLQNRKVVEF--- 57 +E + +QG GVSNF + + + Q+ + +R+ +E+ Sbjct: 125 IEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVL---YNLSRRGIEYDLL 181 Query: 58 --ARSQGIHLTAYMPLAYGKVMADPTIIEIAGAHQANPAQIALAWSLQQ-GFATIPSSTK 114 A+ I + AY P+ G+++ P + +A AH+ PAQIALAW L+Q G IP + Sbjct: 182 PQAQQAHIAVMAYSPIEQGRILKHPALTALAQAHRVTPAQIALAWVLRQPGVVAIPKAAD 241 Query: 115 RANLESNLAAQNIRLSDAEM 134 A++++N A+ +I LS A++ Sbjct: 242 VAHVQANRASLDISLSAADL 261 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.129 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 465,358 Number of extensions: 16898 Number of successful extensions: 61 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 1 Length of query: 156 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 78 Effective length of database: 1,250,540 Effective search space: 97542120 Effective search space used: 97542120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)