BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0086.p (136 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311112|ref|YP_003775204.1| thioesterase [Herbaspirillum se... 170 4e-44 gi|300313596|ref|YP_003777688.1| 4-hydroxybenzoyl-CoA thioestera... 55 2e-09 >gi|300311112|ref|YP_003775204.1| thioesterase [Herbaspirillum seropedicae SmR1] Length = 141 Score = 170 bits (431), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 82/130 (63%), Positives = 96/130 (73%) Query: 7 IHTSRMDVRWGDMDAFNHVNNTVYFRYMEQTRIEWLETIGVGLAADGLGPVIVNAHCSFL 66 ++ SR +RWGDMDAF HVNNTVYFR+MEQ RIEWLE + A GPVIVNAHC+F Sbjct: 12 LYLSRQTIRWGDMDAFGHVNNTVYFRFMEQCRIEWLEEVFGPTIAGEEGPVIVNAHCNFR 71 Query: 67 RQLKYPADIEIRMYAQPPGRSSVETTYEIRRVDQPEIIYAEGGAKIVWVNFKQEKSVPLP 126 RQ+KYPA I + M A GR+SVETTY IR +IIYA+G AKIVWV+FK+EKS LP Sbjct: 72 RQMKYPARICVEMSAGHLGRTSVETTYIIRDAADEQIIYADGAAKIVWVDFKKEKSTALP 131 Query: 127 ESIRQTLTGL 136 E +RQ LT L Sbjct: 132 EQLRQLLTSL 141 >gi|300313596|ref|YP_003777688.1| 4-hydroxybenzoyl-CoA thioesterase [Herbaspirillum seropedicae SmR1] Length = 150 Score = 55.1 bits (131), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 37/133 (27%), Positives = 61/133 (45%), Gaps = 4/133 (3%) Query: 6 LIHTSRMDVRWGDMDAFNHVNNTVYFRYMEQTRIEWLETIGVGLAADG--LGPVIVNAHC 63 +H + RW D D + HVNN VY+ + + +L G G +G +++ C Sbjct: 14 FLHFHAIPTRWMDNDVYGHVNNVVYYSWFDTAVNHYLVQQGALDIEHGEVIG-LVIETQC 72 Query: 64 SFLRQLKYPADIEIRMYAQPPGRSSVETTYEIRRVDQPEIIYAEGGAKIVWVNFKQEKSV 123 ++ + +P IE+ + GRSSV I + + E A G V+V+ + V Sbjct: 73 NYFASVAFPDLIEVGVRVAHLGRSSVRYELGIFKAGE-ESAAARGHFVHVYVDRASRRPV 131 Query: 124 PLPESIRQTLTGL 136 LPE++R L L Sbjct: 132 ALPEALRAALMPL 144 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 443,394 Number of extensions: 17320 Number of successful extensions: 44 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 2 Length of query: 136 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 60 Effective length of database: 1,260,010 Effective search space: 75600600 Effective search space used: 75600600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)