BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0083.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum s... 109 2e-25 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 107 9e-25 gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein... 92 4e-20 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 87 2e-18 gi|300313632|ref|YP_003777724.1| oxidoreductase [Herbaspirillum ... 84 8e-18 >gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 109 bits (273), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 88/262 (33%), Positives = 128/262 (48%), Gaps = 11/262 (4%) Query: 1 MDLALTNKKILITGASQGIGEGLAHAFAQEGCAVYLTARSEEKLQGIAAALRSQYDVAAH 60 M+L L K ++TG S GIG +A A A+EG V + ARS+ KL+ +A+AL Sbjct: 1 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVI 60 Query: 61 VLAADMT----APGAIECLCDFAGDADVLVNNAGAIEGGDLWEIDDARWRGSWE---LKV 113 +AAD++ A+ G D+LVN A G EI A G E +KV Sbjct: 61 AVAADVSNTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLEDINIKV 120 Query: 114 FGYINLTRALYGRMKTRKSGVILNNIGNGGENFDFNYIAGTTGNAALMAFTRAMGGKSLE 173 GY+ +A+ M+ G I+N G G ++G N A+ T+ + + Sbjct: 121 VGYMRFAKAVAPHMRAGGYGRIINIGGLTGRG--SKQLSGMR-NVAICHLTKTLSDQLGP 177 Query: 174 DGIRVVGVNPGPVDTDRIRRVLKKRAGSR-LGDENRYEELLKGYPLGRAAHVREIADMFV 232 DGI V ++PG V+T I + +K A + L E + K P+ R V EIAD+ + Sbjct: 178 DGITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVVL 237 Query: 233 FLASERSGYTSGNIITIDGGIT 254 FLAS R+ +G I +DGGIT Sbjct: 238 FLASPRAAAITGESIAVDGGIT 259 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 107 bits (267), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 81/258 (31%), Positives = 127/258 (49%), Gaps = 24/258 (9%) Query: 5 LTNKKILITGASQGIGEGLAHAFAQEGCAVYLTARSE---EKLQGIAAALRSQYDVAAHV 61 L K +LIT A+QGIG FA+EG V T S+ ++L GIA V H+ Sbjct: 8 LAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELAGIAG-------VETHL 60 Query: 62 LAADMTAPGAIECLCDFAGDADVLVNNAGAIEGGDLWEIDDARWRGSWELKVFGYINLTR 121 L D+T AI+ L G DVL N AG + G++ E DD W S+ L + R Sbjct: 61 L--DVTDDAAIKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIR 118 Query: 122 ALYGRMKTRKSGVILNNIGNGGENFD--FNYIAGTTGNAALMAFTRAMGGKSLEDGIRVV 179 A+ M +K+G I+ NI + + N A AA++ T+++ + GIR Sbjct: 119 AVLPGMLAKKAGSIV-NIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCN 177 Query: 180 GVNPGPVDTDRIRRVLKKRAGSRLGDENRYEELLKG-----YPLGRAAHVREIADMFVFL 234 + PG +++ L +R ++ + + EE ++ P+GR E+A + ++L Sbjct: 178 AICPGTIESPS----LNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYL 233 Query: 235 ASERSGYTSGNIITIDGG 252 AS+ S +T+G+I IDGG Sbjct: 234 ASDESNFTTGSIHMIDGG 251 >gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 92.0 bits (227), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 71/258 (27%), Positives = 115/258 (44%), Gaps = 8/258 (3%) Query: 1 MDLALTNKKILITGASQGIGEGLAHAFAQEGCAVYLTARSEEKLQGIAAALRSQYDVAAH 60 MDL L K L+ +S+G+G AH+ A+EG V + AR E L+ A +R++ Sbjct: 1 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA 60 Query: 61 VL---AADMTAPGAIECLCDFAGDADVLVNNAGAIEGGDLWEIDDARWRGSWELKVFGYI 117 ++ + D+T P D+LV NAG + GD + W + + + I Sbjct: 61 IITTVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSREDWIAAVDANMLTPI 120 Query: 118 NLTRALYGRMKTRKSGVILNNIGNGGENFDFNYIAGTTG-NAALMAFTRAMGGKSLEDGI 176 L +A M R+ G I+ NI + + + + G + L F + K++ + Sbjct: 121 ELIKATVDGMIARRFGRIV-NITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNV 179 Query: 177 RVVGVNPGPVDTDRIRRVLKKRA--GSRLGDENRYEELLKGYPLGRAAHVREIADMFVFL 234 + + PGP +TDR+R A R DE E K P GR E D +L Sbjct: 180 TINNLLPGPFETDRLRATASGAAQESGRSVDEI-LAERRKLNPAGRFGDPAEFGDACAYL 238 Query: 235 ASERSGYTSGNIITIDGG 252 S ++G+ +G + +DGG Sbjct: 239 CSAQAGFITGQNLLMDGG 256 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 83/259 (32%), Positives = 118/259 (45%), Gaps = 26/259 (10%) Query: 5 LTNKKILITGASQGIGEGLAHAFAQEGCAVYLTARSEEKLQGIA---AALRSQYDVAAHV 61 L + L+TGAS+GIG +A A++G V TA SE I AAL + A + Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVL 65 Query: 62 LAADMTAPGAI--ECLCDFAGDADVLVNNAGAIEGGDLWEIDDARWRGSWELKVFGYINL 119 D A A+ + DF G +LVNNAG + + D W + L Sbjct: 66 NVNDAEASVALVEQVQKDF-GSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRL 124 Query: 120 TRA-LYGRMKTRKSGVILN---NIGNGGENFDFNYIAGTTGNAALM-AFTRAMGGKSLED 174 +RA L G MK R +G I+N +G G NY A G A + A R +G ++ Sbjct: 125 SRAVLRGMMKAR-TGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRN--- 180 Query: 175 GIRVVGVNPGPVDTDRIRRVLKKRAGSRLGDENRYEELLKGYPLGRAAHVREIADMFVFL 234 I V V PG +DTD R + +E + +L+ PLGR EIA FL Sbjct: 181 -ITVNCVAPGFIDTDMTRAL----------NEQQSAAILQQIPLGRLGAAEEIAYAVAFL 229 Query: 235 ASERSGYTSGNIITIDGGI 253 AS ++ Y +G + ++GG+ Sbjct: 230 ASPQAAYITGTTLNVNGGM 248 >gi|300313632|ref|YP_003777724.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 84.3 bits (207), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 78/263 (29%), Positives = 122/263 (46%), Gaps = 21/263 (7%) Query: 1 MDLALTNKKILITGASQGIGEGLAHAFAQEGCAVYLTARSEEKLQGIAAALRSQYDVAAH 60 DL L + ++TG +QG+G + AQ G V +ARS LQ + VAA Sbjct: 3 FDLHLNGLRAVVTGGTQGLGAAVVKTLAQAGARVATSARSL-PLQSVEGVTY----VAAD 57 Query: 61 VLAADMTAPGAIECLCDFAGDADVLVNNAGA--IEGGDLWEIDDARWRGSWELKVFGYIN 118 + A+ T + L D+ G D+L+N G GG I D W + L + + Sbjct: 58 LSTAEGTRHFSQTVLKDWGG-VDILINALGGSKTPGGGFAAISDEHWFAEFNLNLMPAVR 116 Query: 119 LTRALYGRMKTRKSGVILNNIGNGGENFDF--NYIAGTTGNAALMAFTRAMGGKSLEDGI 176 + RAL M + SGVI++ + + + A AAL +++++ + G+ Sbjct: 117 MDRALLPNMLAQGSGVIIH-VSSIQRVLPLPESTTAYAAAKAALTTYSKSLAREVTSKGV 175 Query: 177 RVVGVNPGPVDTDRIRRVLKKRAGSRLGDENRYE-------ELLKGYPLGRAAHVREIAD 229 RV+ V PG ++T+ +R + G + YE + L G P+GR A +E+AD Sbjct: 176 RVLSVAPGWIETEA-SVAFTQRTAAHAGTD--YEGGKKIIMDWLGGIPVGRPAKPQEVAD 232 Query: 230 MFVFLASERSGYTSGNIITIDGG 252 + FLAS RS +G IDGG Sbjct: 233 LIAFLASPRSASITGTEYRIDGG 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 880,667 Number of extensions: 39774 Number of successful extensions: 281 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 40 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)