BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0080.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 84 2e-17 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 58 2e-09 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 56 4e-09 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 98/398 (24%), Positives = 173/398 (43%), Gaps = 42/398 (10%) Query: 1 MFTKDSIAKVLAGSTVAALLTCGVSAAHADEIIKLGLSIPLSGAAANWGKGSEWLCNAAA 60 MF + S ++A S L + A +++K+G S PL+G A+ GK ++ A Sbjct: 1 MFKRVSGLNIIAASLA---LIPAFAMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAI 57 Query: 61 KEVADAGGVKVGGKVYNYKCVAYDNKYNAAEGTKVAQTLLNRDGIRFIGGSLGTAPVKAL 120 + + +A + VGGK + +A D++ + G VAQ L ++ G++ I G + Sbjct: 58 ERL-NAQPITVGGKKIKFDVIAEDDQADPKSGVAVAQKLADQ-GVKAIVGPYNSGVTIPA 115 Query: 121 QSLSEREGALHFTTAWGSSIKGPKFPLTFTIMNTVNEISFPLTRFV-KETNPSIKTVSLL 179 + G + T A I F F + + +++ + + KE K V+++ Sbjct: 116 SRVYNDAGIVVATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELK--FKRVAVI 173 Query: 180 NPNDATGQETEALARKAWTAAGVQVISSDWYDRGTTEFQPIASKMAAAKADVIDLASTPP 239 + A GQ K A G+ V+S+D+ + T+F I + + K D + L P Sbjct: 174 DDRTAYGQGLAQEFIKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAP 233 Query: 240 ADSGVVFKELK-------VLGWKGVQAVQVGT-GAEGI-----IATGGTAVDGTYMGAAM 286 G + +++K ++G G+ + ++G G + I GGT +D G Sbjct: 234 -QGGPIKRQMKQLGVDVPLMGGDGICSPEMGRLGGDAIGESVYCTQGGTMLDKAKEG--- 289 Query: 287 VFDSALTTPKQKKLNDAAKAAIGESLNSVQISFYDSVMALKAAMEASQSVDPKLVAAALP 346 + QKK N A+ + +SFYD +M + AM+ + SVDPK AL Sbjct: 290 ---KVFSDEYQKKYNRPAE--------TYAVSFYDGMMLIAQAMKQANSVDPKQFGPALA 338 Query: 347 TVKFESFYGVSSGFGGKDVYGTPQQILIPVIVTQIKNG 384 + S+ GV+ + + Q PV V + K+G Sbjct: 339 KI---SYKGVAGQYDFDANHDLKQS---PVTVYRFKDG 370 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 57.8 bits (138), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 81/334 (24%), Positives = 134/334 (40%), Gaps = 26/334 (7%) Query: 31 EIIKLGLSIPLSGAAANWGKGSEWLCNAAAKEVADAGGVKVGGKVYNYKCVAYDNKYNAA 90 E I G +P+SG A GK ++ A ++ KV GK +Y V D + A Sbjct: 29 ESIAFGCPVPMSGPFAANGKFADLGMKLALQKYG-----KVLGKSTSY--VNLDTEGKPA 81 Query: 91 EGTKVAQTLLNRDGIRFIGGSLGTAPVKALQSLSEREGALHFTTAWGSSIKGPK-----F 145 + Q + + G RF G + + A+ +++ GA+ TTA I G F Sbjct: 82 TAVRKMQESIEK-GTRFFAGGILSGEALAMGKEADKAGAVFITTAGADEITGSDCNRATF 140 Query: 146 PLTFTIMNTVNEISFPLTRFVKETNPSIKTVSLLNPNDATGQETEALARKAWTAAGVQVI 205 + + + PL E P+ K + P G+ + A+ + G++ + Sbjct: 141 RWSVPTFGAIEQTVRPLL----EKMPNAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHV 196 Query: 206 SSDWYDRGTTEFQPIASKMAAAKADVIDLASTPPADSGVVFKELKVLGWKGVQAVQV--G 263 + ++ EF + AAK DV+ L + S + ++ G K + + Sbjct: 197 GNSYHSLTEKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTL-RQAVSFGMKNNCTILMAWA 255 Query: 264 TGAEGIIATGGTAVDGTYMGAAM--VFDSALTTPKQKKLNDAAKAAIGESLNSVQISFYD 321 +G E G DG Y GA D+ K +ND K SL S+ Sbjct: 256 SGLEQFETLGADICDGVYFGAQYWHAIDAPFNKEFVKLVNDNLKIDPNYSLAG---SYIC 312 Query: 322 SVMALKAAMEASQSVDPKLVAAALPTVKFESFYG 355 + + L A ++A+ S DPK V AA+ +K+E G Sbjct: 313 TKIILDAIIKAN-STDPKAVIAAMEGMKYEGLTG 345 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 78/333 (23%), Positives = 145/333 (43%), Gaps = 19/333 (5%) Query: 28 HADEIIKLGLSIPLSGAAANWGKGSEWLCNAAAKEVADAGGVKVGGKVYNYKCVAYDNKY 87 HA E +K+ ++ P++GA A +G + A +++ AGG G K ++ V D+ Sbjct: 25 HA-ETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAGGAN-GNK---FEAVMMDDAC 79 Query: 88 NAAEGTKVAQTLLNRDGIRFIGGSLGTAPVKALQSLSEREGALHFTTAWG----SSIKGP 143 + VA ++++ GI+++ G + + + E EG + T + + K Sbjct: 80 EPKQAVAVANKIVSQ-GIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPH 138 Query: 144 KFPLTFTIMNTVNEISFPLTRFVKET-NPSIKTVSLLNPNDATGQETEALARKAWTAAGV 202 KF F + ++ R+V E P K V++L+ + GQ + + A AA V Sbjct: 139 KF--IFRTIGRDDQQGPAAARYVIEKLKP--KKVAVLHDKQSYGQGVASSVKAALDAAKV 194 Query: 203 QVISSDWYDRGTTEFQPIASKMAAAKADVIDLASTPPADSGVVFKELKVLGWKGVQAVQV 262 V+ + + G +++ I +K+ + D + P + G++ ++ + G K V Sbjct: 195 PVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHP-EMGLIMRQAREQGIKAVFMGPE 253 Query: 263 GTGAEGIIATGGTAVDGTYMGAAMVFDSALTTPKQKKLNDAAKAAIGESLNSVQISFYDS 322 G G + I A G A +G + F P L A AA + Q+ Y Sbjct: 254 GVGNKDITAIAGPASEGMLVTLPADF---AADPANAALVKAFAAAKRDPNGPFQMPAYSG 310 Query: 323 VMALKAAMEASQSVDPKLVAAALPTVKFESFYG 355 V + A+ ++S DP+ VAA + F++ G Sbjct: 311 VKIIADAIAGAKSTDPEKVAAYIHANSFKTPIG 343 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.129 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,063,153 Number of extensions: 38128 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 6 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)