BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0059.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311307|ref|YP_003775399.1| periplasmic polysaccharide expo... 180 1e-46 gi|300312073|ref|YP_003776165.1| outer membrane polysaccharide e... 57 2e-09 >gi|300311307|ref|YP_003775399.1| periplasmic polysaccharide export protein [Herbaspirillum seropedicae SmR1] Length = 264 Score = 180 bits (457), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 95/251 (37%), Positives = 156/251 (62%), Gaps = 7/251 (2%) Query: 49 SSTVTNTRVGSGDVLRVTVFGQPDLSAEVGVNDKGFLTLPLIGGIDVNGLTTSEISAKVA 108 S+ + +G GDV+R+ V+G DL+ E +++ G ++ PLIG + + G++T++ K+A Sbjct: 21 SARAEDIPLGPGDVIRINVYGSQDLTLETRISEAGVISYPLIGEVKIGGMSTAQAERKIA 80 Query: 109 DGLRKGQYLKNPEVSVEVVQLRSQMVSVLGEVSRPGRYPIPGHMSVLELLATAGGLTAQA 168 L++G YL NP+V++ V SQM+SVLG+V +PGRYP+ G +V +++A AGG+ Sbjct: 81 GMLKQGGYLVNPQVNILVTAPASQMISVLGQVYKPGRYPLDGRRNVADVIALAGGVNPDG 140 Query: 169 DQTVTLLRRKNDNNGSSAEIDVRIPILLGETSTTGRTPLDVELNAGDVVNVNKKKLFYIH 228 T+T++R + D + +D+ G+T+ P + A DVV V + + FYI+ Sbjct: 141 GDTITIVRTE-DGKVTRTAVDIYAITHNGDTT---ELP---TVGANDVVYVERYQRFYIY 193 Query: 229 GEVNRPGSYPMEQDMNVMRAISLGGGMTQRASQRRIYINREMPEKGVQEIKAKLTDPILP 288 GEV RPG+Y +E+ V++A++LGGG+T R ++R + + R +QEI K D +LP Sbjct: 194 GEVQRPGAYKLERGTTVLQALTLGGGLTPRGTERGLKVKRRDANGELQEISVKKDDLLLP 253 Query: 289 GDVVYVNESLF 299 D+VYV ES F Sbjct: 254 DDIVYVKESWF 264 >gi|300312073|ref|YP_003776165.1| outer membrane polysaccharide export protein [Herbaspirillum seropedicae SmR1] Length = 206 Score = 56.6 bits (135), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 51/178 (28%), Positives = 89/178 (50%), Gaps = 12/178 (6%) Query: 44 TLALPSSTV-TNTRVGSGDVLRVTVFGQPDLSAEVGVNDKGFLTLPLIGGIDVNGLTTSE 102 T ++P++ V + R+G GD L V V+ P++S V V G + PL I+ +G T E Sbjct: 30 TASVPAAPVNSEYRIGPGDNLNVIVWRNPEVSLVVPVRPDGKFSTPLANDIEASGKTPKE 89 Query: 103 ISAKVADGLRKGQYLKNPEVSVEVV---QLRSQMVSVLGEVSRPGRYPIPGHMSVLELLA 159 + A++A L Y+ +P V+V V + S + V+G+ ++P P MS+L+++ Sbjct: 90 VGAEIAKAL--SAYITDPVVTVVVTGFSGIYSDQIRVIGQAAKPQALPYRKEMSLLDVVI 147 Query: 160 TAGGLTAQADQTVTLLRRKNDNNGSSAEIDVRIPILLGETSTTGRTPLDVELNAGDVV 217 GG T A + R D G +I VR+ L+ + G ++ + GD++ Sbjct: 148 AVGGTTEFAAGNRASVIRLVD--GKQTKIAVRLDDLMKD----GDISANLAMRPGDIL 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.134 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 866,240 Number of extensions: 33723 Number of successful extensions: 120 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 116 Number of HSP's successfully gapped: 2 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)