BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0013.0058.p
         (305 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313077|ref|YP_003777169.1| coenzyme F420-dependent N5,N10-...   186   2e-48
gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent N5,N10-...    66   4e-12

>gi|300313077|ref|YP_003777169.1| coenzyme F420-dependent
           N5,N10-methylene tetrahydromethanopterin reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 336

 Score =  186 bits (472), Expect = 2e-48,   Method: Compositional matrix adjust.
 Identities = 118/301 (39%), Positives = 165/301 (54%), Gaps = 12/301 (3%)

Query: 3   ADRLGYYRYWVAEHHGTQSLACASPEVLIGPIAAATSRLRVGSGGVMLPHYSPLKVAESF 62
           A+ LGY+RYW+AEHH    +A A+  ++I  +AA T  +RVGSGG+MLP++SPL +AE F
Sbjct: 32  AEALGYHRYWLAEHHNMPGIASAATALVISHVAANTRSIRVGSGGIMLPNHSPLVIAEQF 91

Query: 63  SMLSGLYPGRIDLGLGRAPGSDSLTASALQRDRRQRPQDDFPDQLNELLAYLWDELPGDH 122
             L  LYPGRIDLGLGRAPGSD  TA AL+R       D FP  + EL  Y  D  P D 
Sbjct: 92  GTLESLYPGRIDLGLGRAPGSDQRTARALRRHMGGDTADTFPQDVQELQDYFDD--PAD- 148

Query: 123 PFARLPPMPGSPELLQPWMLGSSAQSGIWAAELGMPYMFAD-FIQAAGETVAARYRRDFV 181
            +  L  +PG+   +  W+LGSS  S   AAELG+P+ FA  F     ++    YR  F 
Sbjct: 149 -YQTLRAVPGAGLKVPIWLLGSSMFSAQLAAELGLPFAFASHFAPGFMKSAVQIYRERFK 207

Query: 182 PSPHWPEACQGVALQVICAETDEEAKLLATSYGMRLIHMHSGRRLDKIPSVETALRFFEE 241
           PS    +    +AL V  A+T  EA+ L TS   + +++  G      P V+     ++ 
Sbjct: 208 PSATLKKPYVMLALNVFAADTQREAERLFTSLQQQFMNLIRGTPGQLRPPVDDIDALWQ- 266

Query: 242 HDLPSSTLPSGRR---AVIGSPARVREQIEALAQAYGADEVMIVSIIYDHEARKRSYELI 298
              P       R    AV+G    V++ ++A    +  DE++I + I+DH AR  S+E+ 
Sbjct: 267 ---PGEQAHVARSLACAVVGDRQAVQDGLQAFMDEHAPDELIITAQIFDHAARLHSFEIA 323

Query: 299 A 299
           A
Sbjct: 324 A 324


>gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent
           N5,N10-methylene tetrahydromethanopterin reductase
           [Herbaspirillum seropedicae SmR1]
          Length = 333

 Score = 65.9 bits (159), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 34/79 (43%), Positives = 50/79 (63%)

Query: 1   VLADRLGYYRYWVAEHHGTQSLACASPEVLIGPIAAATSRLRVGSGGVMLPHYSPLKVAE 60
           V+A+ LGY+  WVAEHH +     ++  VL+  IAA TSR+R+G+G  +L    PL+VAE
Sbjct: 33  VVAEALGYHDVWVAEHHYSSFAIGSALMVLLAQIAARTSRIRLGTGASLLALNDPLRVAE 92

Query: 61  SFSMLSGLYPGRIDLGLGR 79
             + L  L  GR++ G+ R
Sbjct: 93  DVATLDLLSNGRMEFGVAR 111


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 997,132
Number of extensions: 41654
Number of successful extensions: 140
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 137
Number of HSP's successfully gapped: 2
Length of query: 305
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 220
Effective length of database: 1,217,395
Effective search space: 267826900
Effective search space used: 267826900
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 130 (54.7 bits)