BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0048.p (142 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 189 9e-50 gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 77 4e-16 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 189 bits (479), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 88/117 (75%), Positives = 106/117 (90%) Query: 1 MAALTAMGALSIDMYLPSFSFIAKDLGVASNTIQLTLSTFLIGLALGQMFYGPISDRFGR 60 +A LTAMGAL+IDMYLPSF+ IA+DLGV +N +QLTL+TFLIG+A+GQMFYGP+SDRFGR Sbjct: 20 LAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYGPLSDRFGR 79 Query: 61 KPPLYFGICLYVVSSIGCTFAPNLETLLVLRFFQGLGGSSGMVIPRAVVRDKMGADG 117 KPPL+FGI LY V S+ C FA N+ETL+VLR FQGLGGS+GMVIPRA+VRD+MGA+G Sbjct: 80 KPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEG 136 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 77.4 bits (189), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 40/113 (35%), Positives = 68/113 (60%), Gaps = 6/113 (5%) Query: 3 ALTAMGALSIDMYLPSFSFIAKDLGVASNTIQLTLSTFLIGLALGQMFYGPISDRFGRKP 62 A + G L+ID+Y+P+ +A D ++ IQ +++ +LI A GQ+ +GP++D +GRK Sbjct: 28 AFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLADAYGRKR 87 Query: 63 PLYFGICLYVVSSIGCTF---APNLETLLVLRFFQGLGGSSGMVIPRAVVRDK 112 L G+ L+ S+GC APNLET + R QG G ++ ++ +A++ D Sbjct: 88 ILALGLGLF---SLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDS 137 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.143 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 424,614 Number of extensions: 16451 Number of successful extensions: 118 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 5 Length of query: 142 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 66 Effective length of database: 1,260,010 Effective search space: 83160660 Effective search space used: 83160660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 126 (53.1 bits)