BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0045.p (364 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312439|ref|YP_003776531.1| molecular chaperone protein [He... 333 2e-92 gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 fami... 227 1e-60 gi|300313992|ref|YP_003778084.1| extracellular solute-binding, f... 150 2e-37 gi|300309950|ref|YP_003774042.1| molecular chaperone protein [He... 110 2e-25 >gi|300312439|ref|YP_003776531.1| molecular chaperone protein [Herbaspirillum seropedicae SmR1] Length = 621 Score = 333 bits (853), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 167/198 (84%), Positives = 183/198 (92%) Query: 167 AQEFTTFKDGQTALAVHVVQGERELVTDCRSLARFELRGIPPMAAGAARIRVTYQVDADG 226 AQEFTTFKDGQTA+A+HVVQGERELV DCRSLARFELRGIPPMAAGAARIRVTYQVDADG Sbjct: 424 AQEFTTFKDGQTAMAIHVVQGERELVADCRSLARFELRGIPPMAAGAARIRVTYQVDADG 483 Query: 227 LLSVSAREMTSGVEAAITVKPSYGLADDDVARMLQDSFQSADEDMKRRALREEQVEAERI 286 LL+VSAREMTSGVEA+ITVKPSYGL DD++ARMLQ+SF SADEDM+RRALREEQVEAERI Sbjct: 484 LLAVSAREMTSGVEASITVKPSYGLGDDEIARMLQESFSSADEDMQRRALREEQVEAERI 543 Query: 287 ILATQSALDADSALLTEQERIDIAALIEIVREKAQSDDHLAIKASVEALAHGTEEFAARR 346 +LAT+SAL AD+ALL+E ER I A +E V AQ DHLAIKA+V+ALAHGTEEFAARR Sbjct: 544 VLATRSALQADAALLSEAERQAIEAQVEAVSAAAQGTDHLAIKAAVDALAHGTEEFAARR 603 Query: 347 MDRSVHAALAGKKLDEVS 364 MDRSVHAALAGKKLDEV+ Sbjct: 604 MDRSVHAALAGKKLDEVA 621 >gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 family [Herbaspirillum seropedicae SmR1] Length = 642 Score = 227 bits (578), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 124/205 (60%), Positives = 151/205 (73%), Gaps = 9/205 (4%) Query: 167 AQEFTTFKDGQTALAVHVVQGERELVTDCRSLARFELRGIPPMAAGAARIRVTYQVDADG 226 AQEFTTFKDGQTA++VHV+QGERELV DCRSLAR ELRGIPPM AGAARIRVT+Q+DADG Sbjct: 435 AQEFTTFKDGQTAMSVHVLQGERELVADCRSLARLELRGIPPMVAGAARIRVTFQIDADG 494 Query: 227 LLSVSAREMTSGVEAAITVKPSYGLADDDVARMLQDSFQSADEDMKRRALREEQVEAERI 286 LLSVSARE ++GVEAA+TVKPSYGL D ++ARML ++ A +DMK R LRE QV A ++ Sbjct: 495 LLSVSAREQSTGVEAAVTVKPSYGLDDANIARMLSEAVGHATDDMKMRMLREAQVNARQV 554 Query: 287 ILATQSALDADSALL---TEQERID-----IAALIEIVREKAQSDDHLAIKASVEALAHG 338 AT +AL D L TEQ I + LIE RE A D+ A+ + +AL Sbjct: 555 ADATDAALKRDGHSLLSATEQAHIRQHLYALTELIETARE-ANDDNIAALGRACDALNQA 613 Query: 339 TEEFAARRMDRSVHAALAGKKLDEV 363 T+EFAARRM+ SV AL+G++LD + Sbjct: 614 TQEFAARRMNASVSLALSGQRLDAI 638 >gi|300313992|ref|YP_003778084.1| extracellular solute-binding, family 1 protein [Herbaspirillum seropedicae SmR1] Length = 473 Score = 150 bits (378), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 70/155 (45%), Positives = 102/155 (65%), Gaps = 4/155 (2%) Query: 1 LANGPFSFGFANSVTTRHPHDWKQRIADGSLDLRNGDAVDIFGRMKLIADKGYLQRGYMN 60 L NGPFS+GFAN V + P DW+Q IA+G+L L N +F R++ + + +Q + Sbjct: 237 LGNGPFSYGFANLVASSTP-DWRQHIANGTLALDNAQGWAVFSRLRTLMTRHLVQADCLR 295 Query: 61 TSYDEGIQLFADGKAAMAFQGTWSSGALMHGKDFSTGVFVPPWNARGKTVVPVVGSETGF 120 YD ++ FA+G+AAM FQG+W++G LM G+ GVFVPPWN + + V+GSETGF Sbjct: 296 AGYDATLRQFAEGRAAMTFQGSWAAGVLMQGRGQPVGVFVPPWNDKDQPRQAVIGSETGF 355 Query: 121 AVC---DTRNRKAALQFLEFIYGAGFAIQQNKRQN 152 A+ R+R+AA +FL++++GAG + Q KRQN Sbjct: 356 AIAQRISARHRQAAARFLDYLFGAGLPVWQGKRQN 390 >gi|300309950|ref|YP_003774042.1| molecular chaperone protein [Herbaspirillum seropedicae SmR1] Length = 646 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 60/179 (33%), Positives = 104/179 (58%) Query: 167 AQEFTTFKDGQTALAVHVVQGERELVTDCRSLARFELRGIPPMAAGAARIRVTYQVDADG 226 +Q F+T D Q A+ + V QGERE+ ++L F L GIPP G +I VT+ +DA+G Sbjct: 424 SQVFSTADDNQPAVTIKVYQGEREMAVGNKALGEFNLEGIPPAPRGTPQIEVTFDIDANG 483 Query: 227 LLSVSAREMTSGVEAAITVKPSYGLADDDVARMLQDSFQSADEDMKRRALREEQVEAERI 286 +L V A++ +G E IT+K + GL ++++ +M++D+ +A+ED + + L E + + + + Sbjct: 484 ILHVGAKDKATGKENKITIKANSGLNEEEIEKMVRDAEANAEEDKRLKELAETRNQGDAL 543 Query: 287 ILATQSALDADSALLTEQERIDIAALIEIVREKAQSDDHLAIKASVEALAHGTEEFAAR 345 + +T+ AL L E+ I A I+ + E + DD AI A EAL+ ++ + Sbjct: 544 VHSTKKALGEYGDKLEAGEKDVIEAAIKNLEEALKGDDKAAIDAKTEALSTAAQKLGEK 602 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,088,012 Number of extensions: 43487 Number of successful extensions: 137 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 4 Length of query: 364 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 278 Effective length of database: 1,212,660 Effective search space: 337119480 Effective search space used: 337119480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)