BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0023.p (379 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312426|ref|YP_003776518.1| nitrogen regulation (sensor his... 670 0.0 gi|300309416|ref|YP_003773508.1| two component sensor histidine ... 85 1e-17 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 73 4e-14 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 73 4e-14 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 70 3e-13 >gi|300312426|ref|YP_003776518.1| nitrogen regulation (sensor histidine kinase) transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 366 Score = 670 bits (1728), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/360 (91%), Positives = 342/360 (95%) Query: 20 TERMKTTLPSLDGLDLLASAVIILDANGGIVYANAAAENLLESSFKVLSQQKLSELFLNG 79 T MKT PSL+GLDLLASAVIILDA GGIVYANAAAENLLESSFKVLSQQKLSELFLNG Sbjct: 7 TTAMKTPFPSLEGLDLLASAVIILDAEGGIVYANAAAENLLESSFKVLSQQKLSELFLNG 66 Query: 80 KELATLFYQAVEHQFADKRLDLVLERIGREPLQVHTIVTALDNADTPVLLELRENVQQLK 139 KEL+ LFYQA+EHQFADKRLDLVLER REPLQVHTIVT LDN D PVLLELRENVQQLK Sbjct: 67 KELSALFYQAIEHQFADKRLDLVLERANREPLQVHTIVTPLDNPDMPVLLELRENVQQLK 126 Query: 140 LDREERLLDQSQANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKELREYTQVIIK 199 LDREER+ DQSQANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKELREYTQVIIK Sbjct: 127 LDREERIFDQSQANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKELREYTQVIIK 186 Query: 200 EADRLQTLVDRLLAPHRRPHIVGDVNIHEVCERVRSLILAEFPNGLTIKRDYDLSIPEFR 259 EADRLQTLVDRLLAPHR PHIVGDVNIHEVCERVRSL++AEFP GLTIKRDYDLSIPEFR Sbjct: 187 EADRLQTLVDRLLAPHRHPHIVGDVNIHEVCERVRSLVMAEFPQGLTIKRDYDLSIPEFR 246 Query: 260 GDKEQLIQALLNIVHNAAQALAERIRTGDGELILQTRVMRQVTLAKVRYRLALDLHIIDN 319 GDKEQLIQA+LNIVHNAAQALA+RIR GDGELIL+TRV RQVTL+KVRYRLALDLHIIDN Sbjct: 247 GDKEQLIQAVLNIVHNAAQALADRIRAGDGELILRTRVSRQVTLSKVRYRLALDLHIIDN 306 Query: 320 GPGISPDIQDRIFYPLVSGREGGSGLGLTLAQTFVQQHMGVIECESRPGCTDFRILIPLP 379 GPGI PDIQDRIFYPLVSGREGGSGLGLTLAQTFV QHMGVIEC+SRPGCTDFRILIPLP Sbjct: 307 GPGIPPDIQDRIFYPLVSGREGGSGLGLTLAQTFVHQHMGVIECDSRPGCTDFRILIPLP 366 >gi|300309416|ref|YP_003773508.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 760 Score = 84.7 bits (208), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 71/237 (29%), Positives = 123/237 (51%), Gaps = 16/237 (6%) Query: 149 QSQANKELIRNLAHEIKNPLGGIRGAAQLLELELPERHLKE----LREYTQVIIKEADRL 204 +S A E+ R LAHEIKNPL I+ +A+ L+ L ++ + L + T I+ + + Sbjct: 522 RSIAWGEVARRLAHEIKNPLTPIQLSAERLQHRLSDKLMPADAAILDKGTTTIVNQVTAM 581 Query: 205 QTLVDRLLAPHRRPHIVG-DVNIHEVCERVRSLILAEFPNGL-TIKRDYDLSIPEFRGDK 262 + +VD R P ++++ + E + +L LA +G I S+ + GD Sbjct: 582 KRMVDDFRDYARTPPAKPVALDLNALIEEILNLYLAG--DGRDAIHARLAPSMAKVMGDA 639 Query: 263 EQLIQALLNIVHNAAQALAERIRTGDGELILQTRVMRQ---VTLAKVRYRLALDLHIIDN 319 QL Q + N++ NA A+A+ +GE+ + V+ + + A + + A+ L I+DN Sbjct: 640 TQLRQVIHNLLQNAQDAVAD-----EGEVAPRIDVLTEQIDIAGADGQRQPAVRLSIMDN 694 Query: 320 GPGISPDIQDRIFYPLVSGREGGSGLGLTLAQTFVQQHMGVIECESRPGCTDFRILI 376 GPG S I R F P V+ + G+GLGL + + V++H G I+ ++R +I+I Sbjct: 695 GPGFSAKILARAFEPYVTSKSKGTGLGLAMVKKIVEEHGGRIDIQNRTDGRGAKIVI 751 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 72.8 bits (177), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 75/247 (30%), Positives = 112/247 (45%), Gaps = 26/247 (10%) Query: 145 RLLDQSQANKELIRNLAHEIKNPLGGIRGAAQLLELE--LPERHLKELREYTQVIIKEAD 202 R + + +A +L +AH+ N L I G QLL+ + L ER R+ ++ I D Sbjct: 718 RQMQKMEAVGQLTGGVAHDFNNVLQIIGGNLQLLQSDPRLDERS----RQRVRIAIGAVD 773 Query: 203 RLQTLVDRLLAPHRR-PHIVGDVNIHEVCERVRSLILAEFPNGLTIKRDYDLSIPEFRGD 261 R L +LLA RR P +N+ V + + L+ + I + D Sbjct: 774 RGAKLSSQLLAFARRQPLQPRSINLERVVQNMDDLLRRAVGENIDISTRVAHDLWNTMLD 833 Query: 262 KEQLIQALLNIVHNAAQALAERIRTGDGELILQTRVMR--------QVTLAKVRYRLALD 313 Q+ ++N NA A+ G L +Q +R Q LA Y ++ Sbjct: 834 PNQVENLIINTAINARDAMPS-----GGRLTVQIDNVRLEDPVFLSQADLAPGEY---VE 885 Query: 314 LHIIDNGPGISPDIQDRIFYPLVSGR-EG-GSGLGLTLAQTFVQQHMGVIECESRPG-CT 370 L I DNG G+SP+++DR F P + + EG G+GLGL++A FV+Q G I S G T Sbjct: 886 LQISDNGAGMSPEVRDRAFEPFFTTKLEGEGTGLGLSMAYGFVKQSKGHISIHSEQGKGT 945 Query: 371 DFRILIP 377 RI P Sbjct: 946 TIRIYFP 952 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 72.8 bits (177), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 74/258 (28%), Positives = 122/258 (47%), Gaps = 20/258 (7%) Query: 136 QQLKLDRE---ERLLDQS---QANKELIRNLAHEIKNPLGGIRGAAQLLELEL--PERHL 187 QL + R E+ L Q+ +A +L +AH+ N L I G ++ L L + L Sbjct: 153 SQLDISRRRDAEQALGQARKMEALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDL 212 Query: 188 KELREYTQVIIKEADRLQTLVDRLLAPHRRPHIVG-DVNIHEVCERVRSLILAEFPNGLT 246 + I K + + L +LLA R+ + G +N++ + E L+ +T Sbjct: 213 ARVARSADSIRKASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDIT 272 Query: 247 IKRDYDLSIPEFRGDKEQLIQALLNIVHNAAQALAER----IRTGDGELILQTRVMRQVT 302 ++ + + R D Q+ ALLN++ NA A+ R IRT + +L R + Q+ Sbjct: 273 VQTELARGLWNCRLDPTQMEVALLNVLINARDAMNGRGRVRIRTANEDLADPDRAV-QLG 331 Query: 303 LAKVRYRLALDLHIIDNGPGISPDIQDRIFYPLVSGR-EG-GSGLGLTLAQTFVQQHMGV 360 L + Y + + + D+GPGI+PDI R+ P + + EG G+GLGL++ FV+Q G Sbjct: 332 LKEGLY---VSIAVTDDGPGIAPDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGS 388 Query: 361 IECESRP-GCTDFRILIP 377 + S G T I P Sbjct: 389 VRITSGAEGGTTVTIYFP 406 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 69.7 bits (169), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 75/290 (25%), Positives = 130/290 (44%), Gaps = 58/290 (20%) Query: 130 ELRENVQQLKLDREERLLDQSQAN-KELIRNLAHEIKNPLGGIRG--------AAQLLEL 180 E R+ ++QLK+ + + + + A+ +L +AHEI NP+G + A LLE Sbjct: 163 EQRKLIEQLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEG 222 Query: 181 ----------ELPERHLKE-LREY-------------TQVIIKEADRLQTLVDRLLAPHR 216 LP +E LR + T+++ + + LQ V ++ + Sbjct: 223 IQLHQCLQGGALPAAMAQEQLRSFEARADLAFLSEDVTELLAESNEGLQR-VRNIVQSLK 281 Query: 217 RPHIVGDVN-----IHEVCERVRSLILAEFPNGLTIKRDYDLSIPEFRGDKEQLIQALLN 271 +G+ N +H + ++ E ++I + Y +P + +L Q +N Sbjct: 282 DFSRIGESNWQMTDLHRGLDSTLNIANNELKYKVSIVKQYG-ELPLVKCLASELNQVFMN 340 Query: 272 IVHNAAQALAERIRTGDGELILQTRVMRQVTLAKVRYRLALDLHIIDNGPGISPDIQDRI 331 ++ NAAQA++ER VT+ R + + I D G GI+P+ +RI Sbjct: 341 LLVNAAQAISER---------------GTVTICTARAGDKVSVSISDTGHGIAPENLNRI 385 Query: 332 FYPLVSGRE--GGSGLGLTLAQTFVQQHMGVIECESRPGC-TDFRILIPL 378 F P + + G+GLGL+L+ V++H G I S PGC T F I +P+ Sbjct: 386 FEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELPI 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,082,478 Number of extensions: 45076 Number of successful extensions: 258 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 20 Length of query: 379 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 292 Effective length of database: 1,207,925 Effective search space: 352714100 Effective search space used: 352714100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)