BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0020.p (430 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 677 0.0 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 394 e-110 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 385 e-108 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 307 1e-84 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 289 2e-79 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 677 bits (1746), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/426 (78%), Positives = 380/426 (89%), Gaps = 1/426 (0%) Query: 6 SSNTGDAKSVKLHPQTWRAVISSSIGNALEWFDVLIYGAFAVIIAKLFFPTENETVSLLV 65 SSNT + HPQ RA+ISSSIGNALEWFD+LIYGAFAV+IAK FFPT +++VSLL+ Sbjct: 2 SSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLL 61 Query: 66 TFATFGVSFFMRPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYASIGLWAP 125 TFATFGVSFFMRPLGAVVLGAYSDR GRKAAL LSI+LMT+GTAMIAFMP+YASIGL AP Sbjct: 62 TFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAP 121 Query: 126 AGIVLARLIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGLSLLMAAVFGAGLNG 185 AGI L ++IQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQG+SLL+AA+FGA LN Sbjct: 122 AGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAALFGAVLNN 181 Query: 186 LLTPEQLYSWGWRLPFLFGLLIGPAGYYIRRHLDESPEFA-AAERTETPLRDTFSSQKMR 244 +LTPEQL SWGWR+PF+FGLLI PAG YIRRHLDE+PEF ++E+T PLRDTF+ QKMR Sbjct: 182 MLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMR 241 Query: 245 LLIGTGSVIMATVSVYLALYMPTYAIKQLGMPAWSSYAATIVSGLIMMVFSPLVGELSDK 304 LLIG GSVIMATVSVYL+LY+PTYA+KQLG+PAWSS+AA V+GLIM + SPLVG LSDK Sbjct: 242 LLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDK 301 Query: 305 YGRTPFMIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPALLADVF 364 GRTPFMI SS ++V TYPMFVFLT++P F+ LLLLQ VIGVLMTMYFA+MPALLAD+F Sbjct: 302 IGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIF 361 Query: 365 PVQTRGTGMSLSYNIAVMLFGGFAGLIITWLIQFTGNKLSVSFFVIFGAALSLIASTAAR 424 PV TRGTGMSL+YNIAV +FGGFAGLIITWLI FTG+KLSVS+FVIFGA LSLIAS +AR Sbjct: 362 PVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSAR 421 Query: 425 YVLRVR 430 VLR+R Sbjct: 422 LVLRLR 427 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 394 bits (1011), Expect = e-110, Method: Compositional matrix adjust. Identities = 202/410 (49%), Positives = 282/410 (68%), Gaps = 3/410 (0%) Query: 18 HPQTWRAVISSSIGNALEWFDVLIYGAFAVIIAKLFFPTENETVSLLVTFATFGVSFFMR 77 + Q RA++++ IGN LEWFD +Y FAVIIAKLFFPT N+ SLL+ ATFGV FFMR Sbjct: 16 NQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMR 75 Query: 78 PLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYASIGLWAPAGIVLARLIQGF 137 P+G ++LG Y+DRVGRKAALS++I+LM +GT MI PTY SIGL+AP IV+ARL+QGF Sbjct: 76 PVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGF 135 Query: 138 SAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGLSLLMAAVFGAGLNGLLTPEQLYSWG 196 SAGGE GS+TAFL E+AP R R ++SSW AS G+++L+ A G + L L SWG Sbjct: 136 SAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWG 195 Query: 197 WRLPFLFGLLIGPAGYYIRRHLDESPEF-AAAERTETPLRDTFSSQKMRLLIGTGSVIMA 255 WRLPFL G++IGP GYYIR HL+E+P F ++T++PL++ + VI+ Sbjct: 196 WRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMVILW 255 Query: 256 TVSVYLAL-YMPTYAIKQLGMPAWSSYAATIVSGLIMMVFSPLVGELSDKYGRTPFMIGS 314 TV Y+ L YMPTY++K L +P + + A +V GL +MV +P+VG L+D+ GR P + GS Sbjct: 256 TVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLLSGS 315 Query: 315 SLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPALLADVFPVQTRGTGMS 374 + ++ YPMF ++ SAPS LL+ Q+V G L+ Y + A +++FP + TG+S Sbjct: 316 AALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLS 375 Query: 375 LSYNIAVMLFGGFAGLIITWLIQFTGNKLSVSFFVIFGAALSLIASTAAR 424 ++YN+AV +FGGFA ITWLI TG+ ++ +F+V+ AA+S + + R Sbjct: 376 VAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVR 425 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 385 bits (988), Expect = e-108, Method: Compositional matrix adjust. Identities = 194/416 (46%), Positives = 288/416 (69%), Gaps = 8/416 (1%) Query: 17 LHPQ--TWRAVISSSIGNALEWFDVLIYGAFAVIIAKLFFPTENETVSLLVTFATFGVSF 74 + PQ R VI++ +GNALEW+D ++YG F+ IIA+LFFP +NE SLLV ATFG+ F Sbjct: 1 MQPQLNKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGF 60 Query: 75 FMRPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYASIGLWAPAGIVLARLI 134 FMRP+G V+LG Y+DR GRKAA+ + IVLMT+ A+IAF PTYA+IG+ AP IV+AR++ Sbjct: 61 FMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARML 120 Query: 135 QGFSAGGEFGSSTAFLVEHAP-HRRGFFSSWQVASQGLSLLMAAVFGAGLNGLLTPEQLY 193 QGF+ GGE+ SSTAFLVE AP H+RG + SWQ+ Q L++ A GA + L+PE L Sbjct: 121 QGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALD 180 Query: 194 SWGWRLPFLFGLLIGPAGYYIRRHLDESPEF--AAAERTETPLR--DTFSSQKMRLLIGT 249 SWGWRLPF+ GLLIGP G +IRRH+ E+ +F +A + P++ D + +L+ Sbjct: 181 SWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSM 240 Query: 250 GSVIMATVSVYLALY-MPTYAIKQLGMPAWSSYAATIVSGLIMMVFSPLVGELSDKYGRT 308 G VI TV+ Y+ L MPT+A KQLG+P ++ +++ ++ P G LSD+ GR Sbjct: 241 GQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRR 300 Query: 309 PFMIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPALLADVFPVQT 368 ++ SSLAF++ YP+FV++++APS LL++Q+++ +++ F MP L++ FP + Sbjct: 301 TVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRV 360 Query: 369 RGTGMSLSYNIAVMLFGGFAGLIITWLIQFTGNKLSVSFFVIFGAALSLIASTAAR 424 R TG++++YN+A MLFGGFA I+TWL + +G+ ++ +++V+F A + + A+ A R Sbjct: 361 RSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMR 416 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 307 bits (786), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 174/393 (44%), Positives = 254/393 (64%), Gaps = 10/393 (2%) Query: 18 HPQTWR-AVISSSIGNALEWFDVLIYGAFAVIIAKLFFPTENETVSLLVTFATFGVSFFM 76 P+T R AVI++++GN LE +D +I+G FAV I+K FFP+ +E +L+ TF TF F + Sbjct: 9 DPKTQRRAVIAATLGNVLEIYDFIIFGIFAVAISKTFFPSGDEYAALMFTFMTFAAGFVV 68 Query: 77 RPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYASIGLWAPAGIVLARLIQG 136 RP+GA+VLG Y+D+VGRK ALS +++LM IGT + A P+Y SIG+ A +V RL+QG Sbjct: 69 RPIGALVLGQYADKVGRKKALSFTLILMAIGTVIPALCPSYDSIGIAATVILVFGRLLQG 128 Query: 137 FSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGLSLLMAAVFGAGLNGLLTPEQLYSW 195 FSAGGE GS+ A LVE+A R R F S+Q +QG +L+ + G + + E++ Sbjct: 129 FSAGGEIGSNVAMLVENASSRNRALFCSFQQMAQGGGVLLGGIVGYVIAVVYGQERMLQD 188 Query: 196 GWRLPFLFGLLIGPAGYYIRRHLDESPEFAAAERTETPLRDTF----SSQKMRLLIGTGS 251 WRL F GL+IGP G+YIRR L E+PEF +++ E+ D+F + R+LIG Sbjct: 189 YWRLAFFVGLVIGPVGWYIRRVLPETPEFERSKKKES--VDSFLEGVKANFGRILIGIAI 246 Query: 252 VIMATVSVYLALYMPTYAIKQLGMPAWSSYAATIVSGLIMMVFSPLVGELSDKYG-RTPF 310 +I TV+ ++ Y TYA+++L MP S+Y I SGL ++ PLVG L+D+ G P Sbjct: 247 MIFWTVATQVSNYFTTYAVRELHMPVASTYLGQISSGLTLLAICPLVGMLADRIGPYKPM 306 Query: 311 MIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPALLADVFPVQTRG 370 G++LA V+ YP+F L + P+ LLL+QI I V + +Y A P +++D+FP + R Sbjct: 307 AFGAALAGVL-AYPLFKMLVATPTLPTLLLVQIAISVTLGLYAACAPLVMSDIFPTRYRA 365 Query: 371 TGMSLSYNIAVMLFGGFAGLIITWLIQFTGNKL 403 TG+ SY + VM+FGG +I+T LIQ +G+KL Sbjct: 366 TGIGTSYALGVMIFGGLTPMIVTTLIQISGDKL 398 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 289 bits (740), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 167/419 (39%), Positives = 253/419 (60%), Gaps = 9/419 (2%) Query: 6 SSNTGDAKSVKLHPQTWRAVISSSIGNALEWFDVLIYGAFAVIIAKLFFPTENETVSLLV 65 ++ T +AK KL V+ + GN LE FD +YG +A I+K FFP++NE VSL++ Sbjct: 2 TTTTTNAKHSKLS-----TVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLIL 56 Query: 66 TFATFGVSFFMRPLGAVVLGAYSDRVGRKAALSLSIVLMTIGTAMIAFMPTYASIGLWAP 125 TF TFG F MRPLGA++LG+Y DRVGR+ L +++ +M IGT +IAF+P YA+IG+ AP Sbjct: 57 TFGTFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAP 116 Query: 126 AGIVLARLIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGLSLLMAAVFGAGLN 184 ++ RL+QGFSAG E G + +L E A P +GF+ SWQ ASQ +++ +A+ G ++ Sbjct: 117 ILVLTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVS 176 Query: 185 GLLTPEQLYSWGWRLPFLFGLLIGPAGYYIRRHLDESPEFAAAERTET--PLRDTFSSQK 242 L+P Q+ WGWR+PF G +I PA + IRR L E+ EF A +R + + + + Sbjct: 177 QSLSPVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNW 236 Query: 243 MRLLIGTGSVIMATVSVYL-ALYMPTYAIKQLGMPAWSSYAATIVSGLIMMVFSPLVGEL 301 ++ G V+M TVS YL +Y PT+ L + A S T GL + P++G L Sbjct: 237 KIVVAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGAL 296 Query: 302 SDKYGRTPFMIGSSLAFVVFTYPMFVFLTSAPSFVNLLLLQIVIGVLMTMYFASMPALLA 361 SD+ GR P ++ +L ++ YP+ +L S SF NLL+ ++ + L Y + L Sbjct: 297 SDRVGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALT 356 Query: 362 DVFPVQTRGTGMSLSYNIAVMLFGGFAGLIITWLIQFTGNKLSVSFFVIFGAALSLIAS 420 +V P R G SL+Y++A +FGGF L+ TWLI+ +G+K + +++ F A L+A+ Sbjct: 357 EVMPAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLAT 415 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,351,168 Number of extensions: 59721 Number of successful extensions: 371 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 330 Number of HSP's successfully gapped: 16 Length of query: 430 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 342 Effective length of database: 1,203,190 Effective search space: 411490980 Effective search space used: 411490980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)