BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0017.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbasp... 763 0.0 gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillu... 177 1e-45 gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillu... 89 4e-19 >gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbaspirillum seropedicae SmR1] Length = 384 Score = 763 bits (1970), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/386 (95%), Positives = 373/386 (96%), Gaps = 2/386 (0%) Query: 1 MSEPQAPATGFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAH 60 MSE QA GFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAH Sbjct: 1 MSEQQA--AGFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAH 58 Query: 61 LLVHGKLPTAAELKAYKAKLKALRGLPANVKAALEWLPAASHPMDVMRTGVSALGCVLPE 120 LLVHGKLPTAAEL+AYKAKLK+LRGLPANVKAALEWLPAASHPMDVMRTGVSALGCVLPE Sbjct: 59 LLVHGKLPTAAELRAYKAKLKSLRGLPANVKAALEWLPAASHPMDVMRTGVSALGCVLPE 118 Query: 121 KDDHNTPGARDIADRLMASLGSMLLYWYHYSQNGKRIEVETDDESIGGHFLHLLHGEKPS 180 KDDHNTPGARDIADRLMASLGSMLLYWYHYS NG RIEVETDD+SIG HFLHLLHGEKPS Sbjct: 119 KDDHNTPGARDIADRLMASLGSMLLYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGEKPS 178 Query: 181 ELWEKAMHTSLILYAEHEFNASTFASRVIAGTGSDMYSAITGGIGALRGPKHGGANEVAF 240 E WEKAMHTSLILYAEHEFNASTF RVIAGTGSDMYSAITG IGALRGPKHGGANEVAF Sbjct: 179 EAWEKAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGANEVAF 238 Query: 241 EIQKRYDNPDEAEADIKRRVENKEVVIGFGHPVYTISDPRNKVIKEVARKLSKDAGSVKM 300 EIQKRYDNPDEAEADI+RRVENKEVVIGFGHPVYTISDPRNKVIKEVARKLSK+AGS KM Sbjct: 239 EIQKRYDNPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKEVARKLSKEAGSTKM 298 Query: 301 FDIAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRI 360 FDIAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRI Sbjct: 299 FDIAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRI 358 Query: 361 DNKIIRPSANYVGPEDLKFVPIGKRK 386 DNKIIRPSANYVGPEDLKFVPI KRK Sbjct: 359 DNKIIRPSANYVGPEDLKFVPISKRK 384 >gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 177 bits (449), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 117/377 (31%), Positives = 196/377 (51%), Gaps = 18/377 (4%) Query: 26 NTALCTVGKTGNDLHYRGYDILDVADSCEFEEIAHLLVHGKLPTAAELKAYKAKLKALRG 85 N+++ + +L YRGY I +A +C+F E +LL+HG+LP A + K + + Sbjct: 81 NSSITYIDGDKGELLYRGYPIEQLAVNCDFLESCYLLLHGELPNAEQKKVFVNTVTQHTM 140 Query: 86 LPANVKAALEWLPAASHPMDVMRTGVSALGCVLPEKDDHNTPGARDI-ADRLMASLGSML 144 + + +HPM V+ V AL + D + R++ A R++A L +++ Sbjct: 141 VHEQMNFFFRGFRRDAHPMAVLTGSVGALSAFYHDSLDISNANHREVSAIRMIAKLPTLV 200 Query: 145 LYWYHYSQNGKRIEVETDDESIGGHFLHLLHGE-----KPSELWEKAMHTSLILYAEHEF 199 Y YS G+ +D S +F+ ++ + +E+ +A+ LIL+A+HE Sbjct: 201 AMAYKYSI-GQPFVYPRNDLSYTANFMRMMFANPCEEYQVNEVLVRALDRILILHADHEQ 259 Query: 200 NASTFASRVIAGTGSDMYSAITGGIGALRGPKHGGANEVAFEIQKRYDNPDEAEADIKR- 258 NAST R+ +G++ ++ I GI L GP HGGANE A + ++ + D+ IK+ Sbjct: 260 NASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSVDKIPEFIKQV 319 Query: 259 RVENKEV-VIGFGHPVYTISDPRNKVIKEVARKLSKDAG--SVKMFDIAERLETVMWD-- 313 + +N V ++GFGH VY DPR K+++E ++ + G +F +A LE + + Sbjct: 320 KDKNSGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAMELEKIALEDE 379 Query: 314 ---IKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRID--NKIIRPS 368 +K++PN+D++S + +G+PT+MFT +F +ART GW A E D KI RP Sbjct: 380 YFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEMISDPEQKIGRPR 439 Query: 369 ANYVGPEDLKFVPIGKR 385 +VG PI KR Sbjct: 440 QLFVGSPRRDVPPIDKR 456 >gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 53/191 (27%), Positives = 93/191 (48%), Gaps = 14/191 (7%) Query: 187 MHTSLILYAEHEFNASTFASRVIAGTGSDMYSAITGGIGALRGPKHGGANEVAFEIQKRY 246 + +L+L A+HE NAS+F SR +A T + + + + G+ L G +HG ++ + Sbjct: 213 IRMALVLCADHELNASSFTSRCVASTDASLRAVVIAGLAGLSGGRHGATTG---RVEALW 269 Query: 247 DNPDEAEAD--IKRRVENKEVVIGFGHPVYTISDPRNKVIKEVARKLSKDAGSVKMFDIA 304 D + D ++ R+ + + GFGH +Y DPR + +++ L + + M D Sbjct: 270 DELGTRDVDRKMRERLSRGDDLPGFGHTLYPDGDPRASYL--LSQILPRHSPWAAMIDAG 327 Query: 305 ERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRIDNKI 364 L P++D+ + +P LF + R+ GW AH +EQR + + Sbjct: 328 ITL-------TGQKPSVDFALVALRRHLRLPVGAAFGLFALGRSIGWIAHGLEQREQHHL 380 Query: 365 IRPSANYVGPE 375 IRP A Y+GP+ Sbjct: 381 IRPRAVYIGPQ 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,253,162 Number of extensions: 51251 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 3 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)