BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0016.p (658 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 1098 0.0 gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 468 e-133 gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 71 2e-13 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 1098 bits (2840), Expect = 0.0, Method: Compositional matrix adjust. Identities = 526/626 (84%), Positives = 578/626 (92%), Gaps = 6/626 (0%) Query: 1 MNTAHRKPLPGTKLDYFDARGAVEAIKPGAWNTLPYTSRVHAENLVRRCDPAILNDYLKQ 60 MNT +RK LPGT+LDYFDAR AVEAI+PGAW+TLPYTSRV AENLVRRCDPA L D L+Q Sbjct: 1 MNTQYRKQLPGTRLDYFDARAAVEAIRPGAWDTLPYTSRVLAENLVRRCDPATLTDSLRQ 60 Query: 61 IIECKRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIADQGGDPAKVNPVVPVQLIVDH 120 +IE KR+LDFPWFPARVVCHDILGQTALVDLAGLRDAIAD GGDPAKVNPVVPVQLIVDH Sbjct: 61 LIERKRELDFPWFPARVVCHDILGQTALVDLAGLRDAIADMGGDPAKVNPVVPVQLIVDH 120 Query: 121 SLAVECGGFDPQAFEKNRAIEDRRNEDRFHFINWTKRAFKNINVIQPGNGIMHQINLEKM 180 SLAVECGG DPQAF KNRAIEDRRNEDRFHFI+WTK AF+N++VI PGNGIMHQINLEKM Sbjct: 121 SLAVECGGDDPQAFVKNRAIEDRRNEDRFHFIDWTKLAFENVDVIPPGNGIMHQINLEKM 180 Query: 181 SPVIHSDNGLAYPDTCVGTDSHTPHVDALGVIAVGVGGLEAENVMLGRASWMRLPEIIGV 240 SPVIH+ +G+A+PDT VGTDSHTPHVDALGVIA+GVGGLEAENVMLGRASWMRLP+IIGV Sbjct: 181 SPVIHAKDGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGV 240 Query: 241 ELSGKPLPGITATDVVLSLTEFLRKEKVVGAYLEFRGEGASALTLGDRATISNMAPEYGA 300 EL+G+ PGITATD+VLSLTEFLRK+KVVGAYLEF G+GA++LTLGDRATISNMAPEYGA Sbjct: 241 ELTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTLGDRATISNMAPEYGA 300 Query: 301 TAAMFFIDQQTIDYLKLTGREDEQVKLVETYAKQAGLWADSLATAQYERTLGFDLSTVVR 360 TAAMF IDQQTIDYLKLTGREDEQVKLVETYAK+AGLW+D+LA +YER L FDLSTVVR Sbjct: 301 TAAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLAKVEYERVLRFDLSTVVR 360 Query: 361 TLAGPSNPHRRLPVSALAERGIAVDLDKAQAQEAEGLMPDGAVIIAAITSCTNTSNPRNV 420 TLAGPSNPH+RLPVS LA +GIA + + G MPDGAVIIAAITSCTNTSNPRNV Sbjct: 361 TLAGPSNPHKRLPVSELAAQGIA-----GKVENEPGKMPDGAVIIAAITSCTNTSNPRNV 415 Query: 421 IAAGLLARNANKLGLTRKPWVKSSLAPGSKTVELYLDEAGLSTELQKLGFGIVAFACTTC 480 IAAGLLARNAN+LGL RKPWVKSSLAPGSK VELYL+EAGL+ +L+KLGFGIVAFACTTC Sbjct: 416 IAAGLLARNANRLGLARKPWVKSSLAPGSKAVELYLEEAGLTADLEKLGFGIVAFACTTC 475 Query: 481 NGMSGALDPVIQKEIIERDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTI 540 NGMSGALDP IQ+EII+RDLY+TAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTI Sbjct: 476 NGMSGALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTI 535 Query: 541 RFDIEKDVLAIV-DGKEIRLKDIWPSDEEIDAVVKSSVKPEQFRNVYTPMFAVQVDKGEA 599 RFDIE+DVL + +G+EIRLKDIWPSDEEIDAVV ++VKP+QFRNVYTPMFA +VD+ ++ Sbjct: 536 RFDIEQDVLGVTAEGREIRLKDIWPSDEEIDAVVTAAVKPQQFRNVYTPMFAARVDRSQS 595 Query: 600 AAPLYDWRPMSTYIRRPPYWEGALAG 625 +PLYDWRP STYIRRPPYWEGALAG Sbjct: 596 VSPLYDWRPQSTYIRRPPYWEGALAG 621 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 468 bits (1203), Expect = e-133, Method: Compositional matrix adjust. Identities = 273/649 (42%), Positives = 394/649 (60%), Gaps = 33/649 (5%) Query: 1 MNTAHRKPLPGTKLDYFDARGAVEAIKPGAWNTLPYTSRVHAENLVRRCD-PAILNDYLK 59 +NT + G+K F + A++ + LP + R+ E+++R CD + +++K Sbjct: 6 LNTLKEFKISGSKKGKFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVTEEHVK 65 Query: 60 QII----ECKRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIADQGGDPAKVNPVVPVQ 115 Q+ + R + P+ ARVV D G L DLA +R+ + G +P + P+VPV Sbjct: 66 QLANWGAKAARVDEIPFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEPLVPVD 125 Query: 116 LIVDHSLAVECGGF-DPQAFEKNRAIEDRRNEDRFHFINWTKRAFKNINVIQPGNGIMHQ 174 L+VDHS+ ++ F + +A + N +E RN +R+ F+ W +AF V+ PG GI+HQ Sbjct: 126 LVVDHSVQIDH--FREKKALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGIVHQ 183 Query: 175 INLEKMSPVIHSDNGLAYPDTCVGTDSHTPHVDALGVIAVGVGGLEAENVMLGRASWMRL 234 +NLE ++ +H+ G+ YPDT VGTDSHT ++ +GV+ GVGG+EAE MLG+ + Sbjct: 184 VNLEYLARGVHNKTGVYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLT 243 Query: 235 PEIIGVELSGKPLPGITATDVVLSLTEFLRKEKVVGAYLEFRGEGASALTLGDRATISNM 294 P+++GV L+G G+TATD+VL++TE LR+ KVVG ++EF GEG +L+L DRATI+NM Sbjct: 244 PDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATIANM 303 Query: 295 APEYGATAAMFFIDQQTIDYLKLTGREDEQVKLVETYAKQAGLWADSLA-TAQYERTLGF 353 APEYGAT F +D TI+Y K TGR ++ E Y K L+ A Y + + Sbjct: 304 APEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGVPKAGQIDYTQEVSL 363 Query: 354 DLSTVVRTLAGPSNPHRRLPVSAL------------AERGI---AVDLDKAQAQEAEGL- 397 DL TV +LAGP P R+ + + AE G A DL A + A+G+ Sbjct: 364 DLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLG-ASYKNADGVD 422 Query: 398 MPDGAVIIAAITSCTNTSNPRNVIAAGLLARNANKLGLTRKPWVKSSLAPGSKTVELYLD 457 + +G V+IAAITSCTNTSNP ++AAGLLA+ A + GL P +K+SLAPGS+ V YL+ Sbjct: 423 LHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKYLE 482 Query: 458 EAGLSTELQKLGFGIVAFACTTCNGMSGALDPVIQKEIIERDLYATAVLSGNRNFDGRIH 517 AGL L+KLGFG+ A+ CTTC G +G L P + + I++ D+ A AVLSGNRNF+ RIH Sbjct: 483 AAGLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEARIH 542 Query: 518 PYAKQAFLASPPLVVAYAIAGTIRFDIEKD-VLAIVDGKEIRLKDIWPSDEEIDAVVKSS 576 P + FLASPPLVVAYAIAG + D+ + V GK+I L DIWP+ +EI+ ++K + Sbjct: 543 PNIRANFLASPPLVVAYAIAGNVTRDLMTEPVGKGKGGKDIYLGDIWPTSQEIEKLLKFA 602 Query: 577 VKPEQFRNVY-----TPMFAVQVDKGEAAAPLYDWRPMSTYIRRPPYWE 620 + + F+ Y P + KG A +Y+W P STYI PP++E Sbjct: 603 MNAKTFKENYADVKGAPGKLWEAIKGVAKGEVYNW-PSSTYIAEPPFFE 650 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 71.2 bits (173), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 102/362 (28%), Positives = 147/362 (40%), Gaps = 64/362 (17%) Query: 183 VIHSDNGLAYPD-TCVGTDSHTPHVDALGVIAVGVGGLEAENVMLGRASWMRLPEIIGVE 241 VI + G P T V DSHT A +A G+G E E+V+ + R + + V+ Sbjct: 110 VIGPEQGATLPGMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQ 169 Query: 242 LSGKPLPGITATDVVLSLTEFLRKEKVVGAYLEFRGEGASALTLGDRATISNMAPEYGAT 301 + G G+TA D+VL++ + G +EF G +L++ R T+ NMA E GA Sbjct: 170 VDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGAR 229 Query: 302 AAMFFIDQQTIDYLKLTGREDEQVKLVETYAKQAGLWADSLATAQYERTL----GFDLST 357 A M D T++YLK GR ++ W +++ Y R+L G Sbjct: 230 AGMVAFDDTTLEYLK--GR---------PFSPSGPHWERAVS---YWRSLHSDPGAKFDM 275 Query: 358 VVRTLAGPSNPHRRLPVSALAERGIAVD-----------------LDKAQAQEAEGLMP- 399 VV A P S E +AVD ++KA A A L P Sbjct: 276 VVTLNAAEIKPQVTWGTS--PEMVVAVDARVPDPDKEKDPTKRDGMEKALAYMA--LKPN 331 Query: 400 ----DGAVIIAAITSCTNTSNPRNVIAAGLLARNANKLGLTRKPWVKSSL-APGSKTVEL 454 D + I SCTN S ++ A + R G R VK ++ PGS V+ Sbjct: 332 TPIEDIRIDKVFIGSCTN-SRIEDLREAAKVVR-----GKFRASNVKLAMVVPGSGLVKE 385 Query: 455 YLDEAGLSTELQKLGFGIVAFACTTCNGMSG-ALDPVIQKEIIERDLYATAVLSGNRNFD 513 + GL + GF C+ C M+ L+P ER + NRNF+ Sbjct: 386 QAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPG------ER-----CASTSNRNFE 434 Query: 514 GR 515 GR Sbjct: 435 GR 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,021,432 Number of extensions: 82511 Number of successful extensions: 239 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 231 Number of HSP's successfully gapped: 3 Length of query: 658 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 567 Effective length of database: 1,188,985 Effective search space: 674154495 Effective search space used: 674154495 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)