BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0175.p (296 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 573 e-165 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 292 2e-80 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 260 9e-71 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 79 4e-16 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 71 1e-13 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 573 bits (1477), Expect = e-165, Method: Compositional matrix adjust. Identities = 268/294 (91%), Positives = 281/294 (95%) Query: 1 MSYVAPLKDMLFVMNELAGLSEVNALPNCEDATPETVEAILEENAKFCSEVVAPLNHSAD 60 MSY AP+KDMLFVMNELAGL+ VNALP CEDATPET EAILEENAKFCSEVVAPLNHSAD Sbjct: 1 MSYTAPVKDMLFVMNELAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSAD 60 Query: 61 KEPSSWSDGKVTTSKGFKEAFKQFGEAGWQGVQHPVEFGGQGLPKLVATPCIEMLNSANL 120 KEPSSW+DGKVTTS GFKEAF+QFGEAGWQGVQHPVEFGGQGLPKL+ATPCIEMLNSANL Sbjct: 61 KEPSSWADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANL 120 Query: 121 SFALCPLLTDGTIEALMTAGTEEQKNTYLTNFISGKWTGTMNLTEPQAGSDLALVRTRAV 180 SFALCPLLTDGTIEALMTAGT++QK T++ NFISGKWTGTMNLTEPQAGSDLALVRTRAV Sbjct: 121 SFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAV 180 Query: 181 PQDDGTFKLSGTKIFITYGDHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVKPDGS 240 P+ DGT+KL GTKIFITYG+HDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLV DGS Sbjct: 181 PEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGS 240 Query: 241 LGERNDVHCVSIEHKLGIKASPTAVLQFGDHGGAVGMLVGEENRGLEYMFIMMN 294 LG RNDVHCVSIEHKLGIKASPTAVLQFGDHGGA+G LVGEENRGLEYMFIMMN Sbjct: 241 LGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMN 294 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 292 bits (747), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 147/295 (49%), Positives = 198/295 (67%), Gaps = 6/295 (2%) Query: 3 YVAPLKDMLFVMNELAGLSEV-NALPNCEDATPETVEAILEENAKFCSEVVAPLNHSADK 61 Y APL+DM FV++EL + EV LP D E V+ +LEE KF S+V+ PLNHS D+ Sbjct: 4 YNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGDR 63 Query: 62 EPSSWS--DGKVTTSKGFKEAFKQFGEAGWQGVQHPVEFGGQGLPKLVATPCIEMLNSAN 119 E + VTT KGFK+A++Q+ EAGW + EFGGQGLP + EMLNS+N Sbjct: 64 EGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSSN 123 Query: 120 LSFALCPLLTDGTIEALMTAGTEEQKNTYLTNFISGKWTGTMNLTEPQAGSDLALVRTRA 179 ++ + P L+ G E L G+EEQK YL +SG+WTGTM LTE G+DL L+R++A Sbjct: 124 QAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSKA 183 Query: 180 VPQDDGTFKLSGTKIFITYGDHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVKPDG 239 PQ DG++K++G KIFI+ G++D++ NI+HLVLAR P+APEG KGISLF+VPKFL DG Sbjct: 184 EPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNADG 243 Query: 240 SLGERNDVHCVSIEHKLGIKASPTAVLQFGDHGGAVGMLVGEENRGLEYMFIMMN 294 SLG RN + C +IE K+GI + T + GA G L+G+ ++GL+ MF+ MN Sbjct: 244 SLGARNPIFCGAIEEKMGIHGNATCQMNL---DGATGWLIGQPHKGLQAMFVFMN 295 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 260 bits (664), Expect = 9e-71, Method: Compositional matrix adjust. Identities = 139/295 (47%), Positives = 179/295 (60%), Gaps = 9/295 (3%) Query: 1 MSYVAPLKDMLFVMNELAGLSEV-NALPNCEDATPETVEAILEENAKFCSEVVAPLNHSA 59 M Y PL+D FV++E+ +V ALP + + + +LEE +F ++VVAPLN Sbjct: 3 MHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIG 62 Query: 60 DKEPSSWSDGKVTTSKGFKEAFKQFGEAGWQGVQHPVEFGGQGLPKLVATPCIEMLNSAN 119 D+E + +G VTT GF+ A+ QF GW + E GGQGLP ++ EML++AN Sbjct: 63 DREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAAN 122 Query: 120 LSFALCPLLTDGTIEALMTAGTEEQKNTYLTNFISGKWTGTMNLTEPQAGSDLALVRTRA 179 + + P L G L G+ + YL SG+W TM LTE QAGSDL +RTRA Sbjct: 123 HGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTRA 182 Query: 180 VPQDDGTFKLSGTKIFITYGDHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVKPDG 239 VPQDDG+++LSG KIFI+ G+HD+ NIVHLVLAR P+AP G KG+SLF+ P+ L DG Sbjct: 183 VPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVL--DDG 240 Query: 240 SLGERNDVHCVSIEHKLGIKASPTAVLQFGDHGGAVGMLVGEENRGLEYMFIMMN 294 S RN VHC IEHK+GI S T L F A LVGE RGL MF+MMN Sbjct: 241 S---RNGVHCTGIEHKMGIHGSATCSLHFEQ---AQAWLVGEAGRGLAAMFVMMN 289 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 79.3 bits (194), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 72/247 (29%), Positives = 114/247 (46%), Gaps = 30/247 (12%) Query: 26 LPNCEDATPETVEAILEENAKFCSEVVAPLNHSADKEPSSWSDGKVTTSKGFKEAFKQFG 85 LP E + A+ E A F +AP D+ D +K+ G Sbjct: 4 LPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMD-----------LWKKLG 52 Query: 86 EAGWQGVQHPVEFGGQGLPKLVATPCIEMLNSANLSFALC-PLLTDGTIEALMTAGTEEQ 144 + G G+ E+GG GL L +E ++ A+ S L ++ + + G EEQ Sbjct: 53 DLGVLGITVSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQ 112 Query: 145 KNTYLTNFISGKWTGTMNLTEPQAGSDLALVRTRAVPQDDGTFKLSGTKIFITYG-DHDM 203 K YL ISG + G + ++EP AGSD+ ++ RA + D + L+G+K++IT G D D Sbjct: 113 KRKYLPRLISGDFIGALAMSEPNAGSDVVSMKLRADKKGD-RYVLNGSKMWITNGPDAD- 170 Query: 204 AENIVHLVLARTPNAPEGVKGISLFIVPKFLVKPDGSLGERNDVHCVSIEHKLGIKASPT 263 V +V A+T + G +G++ F+V K S+ ++ D KLG++ S T Sbjct: 171 ----VLVVYAKT-DLEAGARGMTAFLVEKGY--KGFSVAQKLD--------KLGMRGSHT 215 Query: 264 AVLQFGD 270 L F D Sbjct: 216 GELVFQD 222 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 66/209 (31%), Positives = 101/209 (48%), Gaps = 21/209 (10%) Query: 85 GEAGWQGVQHPVEFGGQGLPKLVATPCIEMLNSANLSFALCPLLTDGTIEALMTAGTEEQ 144 GE G+ G+ P GG GL +L AT E L A S A + + + + G Sbjct: 46 GELGFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSL 105 Query: 145 KNTYLTNFISGKWTGTMNLTEPQAGSDLALVRTRAVPQDDGTFKLSGTKIFIT-YGDHDM 203 K + +G+ G+ LTEP +GSD A ++T A +DG + L+G+K FI+ G D Sbjct: 106 KAQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTA-RLEDGHYVLNGSKAFISGAGSTD- 163 Query: 204 AENIVHLVLARTPNAPEGVKGISLFIVPKFLVKPDGSLGERNDVHCVSIEHKLGIKASPT 263 V +V+ART EG +G+S +VP P S G++ E K+G + PT Sbjct: 164 ----VLVVMARTGG--EGARGVSAIVVPG--NAPGVSYGKK--------ESKMGWNSQPT 207 Query: 264 AVLQFGDHGGAVGMLVGEENRGLEYMFIM 292 + F + L+G+E +G ++F M Sbjct: 208 RTISFDNVKVPADHLLGQEGQG--FIFAM 234 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 976,719 Number of extensions: 42694 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 85 Number of HSP's successfully gapped: 8 Length of query: 296 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 211 Effective length of database: 1,217,395 Effective search space: 256870345 Effective search space used: 256870345 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)