BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0174.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 453 e-129 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 176 2e-45 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 134 7e-33 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 66 3e-12 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 453 bits (1165), Expect = e-129, Method: Compositional matrix adjust. Identities = 232/312 (74%), Positives = 263/312 (84%), Gaps = 9/312 (2%) Query: 1 MFI---AARFAVGVQGISVAERAYQKAVAYAKDRVQSRDLAGSAGAVTIIHQPDVRRMLM 57 MFI AARFAVG+QG+SV+ERAYQKAVAYA+DR+QSRDLAGS G V IIHQPDVRRMLM Sbjct: 289 MFIMMNAARFAVGIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLM 348 Query: 58 SMRSQVEASRALAYVAAAASDAGHHHADEATRKTNQAVYEYLVPIVKGWSTELSIDVTST 117 SMR+Q+EA+RAL+YVAAA SDA H DEATRK+NQA YEYLVPIVKGW TE+SIDVTST Sbjct: 349 SMRAQIEAARALSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTST 408 Query: 118 GVQVHGGMGFIEETGAAQYYRDARILAIYEGTTAIQANDLVGRKTSRDGGAVAKGIIGQI 177 GVQVHGGMGFIEETGAAQYYRDARIL IYEGTTAIQANDLVGRKT+RDGGAVAKGI+ Q+ Sbjct: 409 GVQVHGGMGFIEETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQV 468 Query: 178 HKTEAALATAAAGEYSDDLQAIRKRLVDGTLALDDVVNYVVANIKTDIKGVFSGSVPYLK 237 KT + L A LQ I RL + AL+ VV+YVVAN+K+DIK VF+GSVPYLK Sbjct: 469 RKTVSELQGDA------QLQPIAARLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLK 522 Query: 238 LAGVVLGGWQMARAALVSVDKLKAGDGDAQFYKAKIATARFYADHVLTQASSLRAAVVEG 297 +AGVVLGGWQMARAA VS KLKAG+GD +FY+AK+ATARFYAD +L QA + R A+V+G Sbjct: 523 MAGVVLGGWQMARAAAVSAAKLKAGEGDGKFYEAKLATARFYADQILPQALAYRTAIVDG 582 Query: 298 SAGVLALTEEQF 309 A V+AL EEQF Sbjct: 583 GAAVMALAEEQF 594 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 176 bits (447), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 122/322 (37%), Positives = 173/322 (53%), Gaps = 28/322 (8%) Query: 1 MFI---AARFAVGVQGISVAERAYQKAVAYAKDRVQSRDLAG----SAGAVTIIHQPDVR 53 MF+ AAR VG+QG+ + E AYQ A+ YAKDR+Q R L+G A II PDVR Sbjct: 290 MFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVR 349 Query: 54 RMLMSMRSQVEASRALAYVAAAASDAGHHHADEATRKTNQAVYEYLVPIVKGWSTELSID 113 RML++ ++ E RA A D H DE RK + + L P+VK + T+ + Sbjct: 350 RMLLTAKAFAEGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWM 409 Query: 114 VTSTGVQVHGGMGFIEETGAAQYYRDARILAIYEGTTAIQANDLVGRKTSRDGGAVAKGI 173 TS +QV+GG G+I E G QY RDARI IYEGT IQ+ DL+GRK D GA K Sbjct: 410 ATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKF 469 Query: 174 IGQI------HKTEAALATAAAGEYSDDLQAIRKRLVDGTLALDDVVNYVVANIKTDIKG 227 QI H T+ +++ E+ L I ++ T+ ++ AN D G Sbjct: 470 GAQIQAFVEEHGTDESMS-----EFITPLADIGDKVTKLTM---EIGMKSFAN--QDEAG 519 Query: 228 VFSGSVPYLKLAGVVLGGWQMARAALVSVDKLKAGDGDAQFYKAKIATARFYADHVLTQA 287 + +VPYL++ G ++ + AR A ++++K +GD FY AK+ATARFY +L + Sbjct: 520 --AAAVPYLRVVGHLVFSYLFARMAKIALEKQDSGD---SFYAAKLATARFYFARLLPET 574 Query: 288 SSLRAAVVEGSAGVLALTEEQF 309 + L G+A ++AL E F Sbjct: 575 AMLIRQARSGAANLMALDAELF 596 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 134 bits (338), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 72/177 (40%), Positives = 100/177 (56%), Gaps = 1/177 (0%) Query: 1 MFIAARFAVGVQGISVAERAYQKAVAYAKDRVQSRDLAGSAGAVTIIHQPDVRRMLMSMR 60 M AAR VG QG+ +AE AYQ+A AYA++R QSR GS+G I+H P V+++LM R Sbjct: 287 MMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRA-PGSSGNDLIVHHPAVQKLLMEQR 345 Query: 61 SQVEASRALAYVAAAASDAGHHHADEATRKTNQAVYEYLVPIVKGWSTELSIDVTSTGVQ 120 ++E +R L A D H + A R + E++ P++K + T+ S +Q Sbjct: 346 CRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQ 405 Query: 121 VHGGMGFIEETGAAQYYRDARILAIYEGTTAIQANDLVGRKTSRDGGAVAKGIIGQI 177 V GG G++ ETG Q+ RDARI IYEGT IQA D + RK D G +G + Sbjct: 406 VFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLV 462 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 46/162 (28%), Positives = 76/162 (46%), Gaps = 22/162 (13%) Query: 4 AARFAVGVQGISVAERAYQKAVAYAKDRVQSRDLAGSAGAVTIIHQPDVRRMLMSMRSQV 63 A R + Q + +A A++ AV+YA+ R + V II V L M + + Sbjct: 239 AGRIGIAAQSVGMARAAFEAAVSYARQR--------ESFGVPIIEHQAVNFRLADMNTLL 290 Query: 64 EASRALAYVAAAASDAGHHHADEATRKTNQAVYEYLVPIVKGWSTELSIDVTSTGVQVHG 123 +A+R + + AA D G EA+ + K +++E + + S +Q+HG Sbjct: 291 DAARLMVWRAAQLKDQGRPCLKEAS-------------MAKMFASEAAEKIASDAIQIHG 337 Query: 124 GMGFIEETGAAQYYRDARILAIYEGTTAIQANDLVGRKTSRD 165 G+G+ + + YRD RI IYEG IQ ++GR + D Sbjct: 338 GVGYTSDFPVERIYRDVRICQIYEGANDIQ-RLVIGRAIASD 378 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 817,369 Number of extensions: 31050 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 5 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)