BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0158.p (292 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 434 e-123 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 221 4e-59 gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 187 7e-49 gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 137 1e-33 gi|300311514|ref|YP_003775606.1| two component sensor histidine ... 115 3e-27 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 434 bits (1116), Expect = e-123, Method: Compositional matrix adjust. Identities = 223/286 (77%), Positives = 240/286 (83%), Gaps = 6/286 (2%) Query: 2 GPS---GSPYILLFLVLIAMLAYVVARMAARPIKHLASAATELGRDIDHPPLAEKGPTEI 58 GP+ G YILLFLVLIAMLA++VARMA RPI+ LA AA+ LG DIDH PLAE GPTEI Sbjct: 175 GPASLFGLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEI 234 Query: 59 RQAAKAFNAMQTRIRRQIQHRTHMLAAITHDLQTPLTRLRLRLEKVEDKELQQKLVDDLA 118 RQAA AFNAMQ RIRRQIQHRTHMLAAITHDLQTPLTR+RLRLEKV D LQQKL+DDL Sbjct: 235 RQAAHAFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVSDPGLQQKLIDDLG 294 Query: 119 VMQDMVREGLDLARSMDSSEAMQRLDIDSLLDSVCADASDAGQQVELQGRTRAFVVAQPN 178 VM MVREGLDLARSMDSSE +Q LDIDSLLDSVCADA+DAGQ V LQG TRAFV+AQP Sbjct: 295 VMHGMVREGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVMAQPG 354 Query: 179 ALRRCLTNLLDNAFKYGQRAEVSIALDDLLPNRVLIRIRDHGPGIPEGQLETVFEPFYRL 238 ALRRCLTNL+DNA KYG+ A VSIAL+ ++ IRIRD GPGIPE +LE VFEPFYRL Sbjct: 355 ALRRCLTNLIDNACKYGKSARVSIALEQ---QKIAIRIRDQGPGIPESELEAVFEPFYRL 411 Query: 239 ETSRSRDTGGTGLGLTIARNIAENHGALLDLRNHAKGGLEVLLSLP 284 E SRSRDTGGTGLGLTIA NIAENH L LRN A+GGLEVLL LP Sbjct: 412 EASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELP 457 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 221 bits (564), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 119/274 (43%), Positives = 175/274 (63%), Gaps = 4/274 (1%) Query: 11 LFLVLIAMLAYVVARMAARPIKHLASAATELGRDIDHPPLAEKGPTEIRQAAKAFNAMQT 70 L + + ++A++ R+A +P++ +A AA +LG +I PLA GP E+ AA+AFNAMQ Sbjct: 209 LRIFAVVLIAWMAVRLAMKPLQVMAEAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQR 268 Query: 71 RIRRQIQHRTHMLAAITHDLQTPLTRLRLRLEKVEDKELQQKLVDDLAVMQDMVREGLDL 130 RI I RT LAA++HDL++P+TR++LR E + D+ ++K DL M ++ L++ Sbjct: 269 RIIDNIAERTRFLAAVSHDLRSPITRMKLRTEMLPDERGKEKFRKDLDEMDALISATLNV 328 Query: 131 ARSMDSSEAMQRLDIDSLLDSVCADASDAGQQVELQGRTRAFVVAQPNALRRCLTNLLDN 190 + +D E Q +D+DSLL+S+ D ++ G +V L G A + P +LRRCL NL++N Sbjct: 329 VQDIDVQERPQLIDLDSLLESLRTDQAEVGGEVTLSGHA-APLQGYPRSLRRCLQNLVEN 387 Query: 191 AFKYGQRAEVSIALDDLLPNRVLIRIRDHGPGIPEGQLETVFEPFYRLETSRSRDTGGTG 250 A +YGQRA V + D ++V IR+ D GPGIPE LE V EPFYRLE SR+ TGG G Sbjct: 388 AVRYGQRARVQLQDD---ADQVCIRVDDDGPGIPEALLERVQEPFYRLERSRNAGTGGFG 444 Query: 251 LGLTIARNIAENHGALLDLRNHAKGGLEVLLSLP 284 LGL+IA + + H ++ L N GGL V + +P Sbjct: 445 LGLSIAATVVQAHRGVMKLVNRPGGGLRVEILIP 478 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 187 bits (476), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 109/267 (40%), Positives = 158/267 (59%), Gaps = 10/267 (3%) Query: 23 VARMAARPIKHLASAATELGRDIDHPPLAEKGPTEIRQAAKAFNAMQTRIRRQIQHRTHM 82 V R RP+ LA AA G+ P L E G E + A+AF+ M+ RI+R ++ R + Sbjct: 215 VVRWTTRPLAALADAAEAFGKGETVPSLPETGSREYVKTARAFSGMRERIKRYLEDRERL 274 Query: 83 LAAITHDLQTPLTRLRLRLEKVEDKELQQKLVDDLAVMQDMVREGLDLARSMDSSEAMQR 142 +I+HDL+TP+TRL+LR E ++D ++ DDL + MV+ L + D E Sbjct: 275 FVSISHDLRTPITRLKLRTELMDDDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRTE 334 Query: 143 LDIDSLLDSVCADASDAGQQVELQGRTRAFVVAQPNALRRCLTNLLDNAFKYGQRAEVSI 202 + +D+L+ + DA AG ++ + V+A+P AL+R + NL DNA YGQ+ E+SI Sbjct: 335 VRLDALVLRMIRDARLAGHEIAFT-PSGINVIAKPLALKRAIGNLFDNALYYGQKVEISI 393 Query: 203 -----ALDDLLPNRVLIRIRDHGPGIPEGQLETVFEPFYRLETSRSRDTGGTGLGLTIAR 257 A DL V I IRDHGPG+PE + T+F+P+ RLE R++++GG GLGL I+R Sbjct: 394 RPSPGAEGDL----VEIEIRDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGMGLGLGISR 449 Query: 258 NIAENHGALLDLRNHAKGGLEVLLSLP 284 NI + HG L LRNH +GGL + LP Sbjct: 450 NIVQAHGGELLLRNHPEGGLMATIILP 476 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 137 bits (345), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 98/294 (33%), Positives = 155/294 (52%), Gaps = 12/294 (4%) Query: 5 GSPYILLFLVLIAMLAYVVARMAARPIKHLASAATELGRDIDHPPLAEKGPTEIRQAAKA 64 G I LFL LI A ++ + +P+ L +AA + + PL E GPTEI +A ++ Sbjct: 164 GWGSIALFLSLIG--AVFISTLINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRS 221 Query: 65 FNAMQTRIRRQIQHRTHMLAAITHDLQTPLTRLRLRLEKVE-DKELQQKLVDDLAVMQDM 123 FN M + R R +LA I+HDL+TPL R++L +E E + + DLA M + Sbjct: 222 FNQMVADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSI 281 Query: 124 VREGLDLARSMDSSEAMQRLDIDSLLDSVCADASDAGQ-QVELQGRTRAFVVAQPNALRR 182 + + LD A+ D+S +++ +D+ +L V +AS +V Q V L R Sbjct: 282 IGQFLDYAKPFDTS-SLETIDLADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTELAR 340 Query: 183 CLTNLLDNAFKYGQRAEVSIALDDLLP----NRVLIRIRDHGPGIPEGQLETVFEPFYRL 238 +NL++NA +YG+ A D+ ++V++ + DHGPG+P + E + PF RL Sbjct: 341 VFSNLVENARRYGKTEGTDCAEIDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPFTRL 400 Query: 239 ETSRSRDTGGTGLGLTIARNIAENHGALLDLRNH--AKGGLEVLLSLPLKQAKR 290 +T+R + G+GLGL I I H L L+ H + GL V ++LPL+ +R Sbjct: 401 DTARGQ-ANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLPLQGHRR 453 >gi|300311514|ref|YP_003775606.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 473 Score = 115 bits (289), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 86/308 (27%), Positives = 162/308 (52%), Gaps = 26/308 (8%) Query: 7 PYILLFLVLIAMLAY--VVARMAARPIKHLASAATELGR---DIDHPPLAEKGPTEIRQA 61 P + + + L+A L + ++A + PI HL +A + D++ P+ E+ Sbjct: 164 PTMPIVVALLASLVFALLLAWYFSHPIGHLKNAFNQAANGNLDLELEPVMSARNDELADL 223 Query: 62 AKAFNAMQTRIRRQIQHRTHMLAAITHDLQTPLTRLR--LRLEKVEDKE----LQQKLVD 115 + F+ M ++R ++ + +L ++H+L++P+ RL+ + L +V+DK+ +++ Sbjct: 224 GRDFDVMTAKLRTLMESQRRLLHDVSHELRSPIARLQVAIGLARVQDKDKLDLTLERIER 283 Query: 116 DLAVMQDMVREGLDLAR-------SMDSSEAMQRLDIDSLLDSVCADASDAGQQVELQGR 168 + M +V E L L+R SM+ A++ + + +++ +A G+ V+ Q Sbjct: 284 ESVRMDKLVGELLTLSRLEANVPGSMEEPIAIEAM-LGDMINDASFEAEAIGKHVDDQLD 342 Query: 169 TRAFVVAQPNALRRCLTNLLDNAFKY---GQRAEVSIALDDLLPNRVL-IRIRDHGPGIP 224 R V P L R + N+L NA K+ G ++ LD++ N++L + I D GPG+P Sbjct: 343 CRVQVKGNPELLYRAIENVLRNAIKHTAPGTTVQIHTQLDEI--NKLLHLSILDRGPGVP 400 Query: 225 EGQLETVFEPFYRLETSRSRDTGGTGLGLTIARNIAENHGALLDLRNHAKGGLEVLLSLP 284 E ++ ++FE F+R ++S ++ G GLGL+IAR I E H + N + GGL V ++LP Sbjct: 401 EEEMNSIFELFFRSSRTKS-NSSGHGLGLSIARRIVEAHQGFIQAANRSNGGLRVDITLP 459 Query: 285 LKQAKRAR 292 + AR Sbjct: 460 FMADEPAR 467 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 812,348 Number of extensions: 31578 Number of successful extensions: 261 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 39 Length of query: 292 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 208 Effective length of database: 1,222,130 Effective search space: 254203040 Effective search space used: 254203040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)