BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0157.p (153 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 159 8e-41 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 159 bits (403), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 75/133 (56%), Positives = 99/133 (74%), Gaps = 6/133 (4%) Query: 8 LREFVGSIGNRVFLILLAGILVATVTTTWLASNERRNTMRELRYQHVAERVEQVVQALDP 67 ++ F+GSIGNR+F +LL GI+VA T WLA NERRNT+RELR+QH+A+RVEQ+VQA+D Sbjct: 1 MKNFLGSIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAVDN 60 Query: 68 LTPQARDVVLHAADNFGFEAGFVNEVGDM------QSPSTGPMADMLKSRLGEDRQIVVQ 121 + P R +VL AA NFGFEA V ++ D SPST P+ +ML++RLGEDR+I VQ Sbjct: 61 IPPAQRPIVLQAASNFGFEARMVEDMNDAIGPDASPSPSTSPLTEMLQARLGEDRKIAVQ 120 Query: 122 RETDCAPRRPRNA 134 RE++C P R +A Sbjct: 121 RESECPPLRTDDA 133 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 395,448 Number of extensions: 14123 Number of successful extensions: 59 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 58 Number of HSP's successfully gapped: 1 Length of query: 153 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 76 Effective length of database: 1,255,275 Effective search space: 95400900 Effective search space used: 95400900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)