BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0153.p (500 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 634 0.0 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 272 4e-74 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 163 2e-41 gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 148 1e-36 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 126 5e-30 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 634 bits (1634), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/422 (73%), Positives = 361/422 (85%), Gaps = 8/422 (1%) Query: 64 PVGVAVARISDVNIYLTGLGGVTPTATATVRSRVDGQLMKLHFKEGQLVKEGDLLAELDA 123 PVGVA AR+ DVN++L GLG VTPTATATVR+RVDGQLMKLH+KEGQ+VK GDLLAE+D Sbjct: 33 PVGVAKARLQDVNVFLNGLGAVTPTATATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDP 92 Query: 124 RPYQVAVTQAEGQLARDQAQLGAARLDLQRYRTLLSQDSIASQQVDTQAALVKQLEGTVK 183 R Q A+TQAEGQLARD+A L +ARLDL+RY+TLL+QDSIASQQVDTQ ALVKQ EGTVK Sbjct: 93 RSLQAALTQAEGQLARDRALLASARLDLKRYQTLLAQDSIASQQVDTQVALVKQYEGTVK 152 Query: 184 SDQGNLASARLQLTYSRVTAPVSGRLGLRQVDIGNIVHSSDTNGIVIITQVQPITAVFSI 243 +D+GN+ASARLQL+++RVTAP+SGRLGLRQ D+GN V +SDTNG+VIITQ+QPITA+FSI Sbjct: 153 ADEGNVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDTNGLVIITQLQPITAIFSI 212 Query: 244 PEDNIPAVMKQLQAGKKLPTDAWDRDQKNKLASGMLLTIDNQVDATTGTIKLKAQFPNTD 303 PEDNIP V++QLQ+G+KLP AWDR+QKNKLA G+LLTIDN VDATTGT+KLKAQFPNTD Sbjct: 213 PEDNIPKVLQQLQSGRKLPAQAWDREQKNKLADGVLLTIDNVVDATTGTVKLKAQFPNTD 272 Query: 304 YALFPNQFVNIRMLLDTRKDAVTIPTAALQRGSKGLFVYVVKSDNTVTVRNVKTGPAQGD 363 YALFP+QFVNIR+ L+T + A IPTAA+QRGSKGLFVYVVK D++VTVR VKTGP Q D Sbjct: 273 YALFPSQFVNIRLQLNTEQGATVIPTAAIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDD 332 Query: 364 ITAIEDGVASGETVVVDGIDRLREGAKVEPVVRGGADDPANKLTEKAGPRRHKRDGAAG- 422 +T I DGV++GETVV+DGIDRLREGAKVE V RGGADDPANKLT + RRH + G Sbjct: 333 LTVITDGVSAGETVVIDGIDRLREGAKVEAVARGGADDPANKLTTENPERRHGKRGQGNP 392 Query: 423 -------EAQSGQGGQDMTPEERQKRWAELNKRIDAGEFGEEIKKLPEDQRRQRMMEMRR 475 M+PEERQKRWAELNKRIDAGEFGEEIKKLPEDQRRQ+MME+RR Sbjct: 393 GAQAGAGGDAGQGAQGGMSPEERQKRWAELNKRIDAGEFGEEIKKLPEDQRRQKMMELRR 452 Query: 476 QQ 477 Q+ Sbjct: 453 QR 454 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 272 bits (696), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 158/376 (42%), Positives = 241/376 (64%), Gaps = 30/376 (7%) Query: 22 WHH-KRAAAAEQAQNGGGQWQGGGGKGKRGGPPGGGFGGMGLSPVGVAVARISDVNI--Y 78 W H +RA+AA+Q Q PP PV V +A + + ++ Y Sbjct: 29 WTHLQRASAADQPQ--------------AKAPP----------PVSVTLASVQERDLPQY 64 Query: 79 LTGLGGVTPTATATVRSRVDGQLMKLHFKEGQLVKEGDLLAELDARPYQVAVTQAEGQLA 138 L G+G VT A+ V++R+DGQL K+ F+EG+ VK+G LLA++DAR Q + QAE Q A Sbjct: 65 LAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAEAQRA 124 Query: 139 RDQAQLGAARLDLQRYRTLLSQDSIASQQVDTQAALVKQLEGTVKSDQGNLASARLQLTY 198 +D A L ARLDLQRY TL SQD+ QQ+DTQ ALV QLE VK+D+ +A A++QL+Y Sbjct: 125 KDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKVQLSY 184 Query: 199 SRVTAPVSGRLGLRQVDIGNIVHSSDTNGIVIITQVQPITAVFSIPEDNIPAVMKQLQAG 258 + + AP+SGR+G R VD GNIVH++D NG+V+I Q+ PIT VF++PE+ + + + +A Sbjct: 185 TTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRAQRAH 244 Query: 259 KK-LPTDAWDRDQKNKLASGMLLTIDNQVDATTGTIKLKAQFPNTDYALFPNQFVNIRML 317 ++ L A+ RD + LA+G L+ +DNQ++ T GT++LKA+F N D+ L+P Q+VN R+ Sbjct: 245 QQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNARLQ 304 Query: 318 LDTRKDAVTIPTAALQRGSKGLFVYVVKSDNTVTVRNVKTGPAQGDITAIEDG--VASGE 375 + + +A+T+P AA+QR +G +VY + ++ ++ VK Q I + G V +G+ Sbjct: 305 MRSHGNALTVPAAAIQRNQQGTYVYAIDQEDQARMQPVKVERIQDGIAVLAAGNAVQAGQ 364 Query: 376 TVVVDGIDRLREGAKV 391 VV+DG +L+ G+++ Sbjct: 365 RVVIDGQYKLKPGSRI 380 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 163 bits (413), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 115/352 (32%), Positives = 182/352 (51%), Gaps = 11/352 (3%) Query: 62 LSPVGVAVARISDVNIYLTGL--GGVTPTATATVRSRVDGQLMKLHFKEGQLVKEGDLLA 119 + P V+V I+ + +T G V A VR+RV G + K FKEG VKEG +L Sbjct: 34 MPPSEVSVVTIAPQRVAITNELPGRVEAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLF 93 Query: 120 ELDARPYQVAVTQAEGQLARDQAQLGAARLDLQRYRTLLSQDSIASQQVDTQAALVKQLE 179 ++D Y+ ++ A+ LAR QA L L QRY+ L+ ++++ Q+ D A KQ E Sbjct: 94 KIDPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAE 153 Query: 180 GTVKSDQGNLASARLQLTYSRVTAPVSGRLGLRQVDIGNIVHSSDTNGIVIITQVQPITA 239 V + + + +A L L Y+ VT+P+SGR+G V G +V D + ++ Q+ P+ Sbjct: 154 ADVAAGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDPVYV 213 Query: 240 VFSIPEDNIPAVMKQLQAG--------KKLPTDAWDRDQKNKLASGMLLTIDNQVDATTG 291 + ++ + + + AG K T + Q L G LL D VD TG Sbjct: 214 NVTQSSADLLKLQQAMAAGQLKSVGDNKASVTLELENGQAYPL-PGKLLFSDISVDENTG 272 Query: 292 TIKLKAQFPNTDYALFPNQFVNIRMLLDTRKDAVTIPTAALQRGSKGLFVYVVKSDNTVT 351 ++ L+A+FPN L P +V ++ + A+ +P A+ R + G V VV ++N V Sbjct: 273 SVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRDAGGSTVMVVNAENKVE 332 Query: 352 VRNVKTGPAQGDITAIEDGVASGETVVVDGIDRLREGAKVEPVVRGGADDPA 403 R VKTG A+G+ + DG+ G+ V+V+G+ ++R GA V+PV A PA Sbjct: 333 PRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATVKPVQWNPAGAPA 384 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 148 bits (373), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 101/305 (33%), Positives = 159/305 (52%), Gaps = 2/305 (0%) Query: 93 VRSRVDGQLMKLHFKEGQLVKEGDLLAELDARPYQVAVTQAEGQLARDQAQLGAARLDLQ 152 VR V G L +HFK+G LVK+GD+L +D RPY+ V +AE QLA +A+ G DL Sbjct: 79 VRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEARAGYTASDLA 138 Query: 153 RYRTLLSQDSIASQQVDTQAALVKQLEGTVKSDQGNLASARLQLTYSRVTAPVSGRLGLR 212 R + LLS ++IA + + + ++ V + + L +ARL L Y+R+TAPVSGR+ Sbjct: 139 RGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTRITAPVSGRISRA 198 Query: 213 QVDIGNIVHSSDTNG-IVIITQVQPITAVFSIPEDN-IPAVMKQLQAGKKLPTDAWDRDQ 270 +V +GN+V+ N + + V + A F + E + + V AG +P + ++ Sbjct: 199 EVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVNPARAAGATVPVELGLANE 258 Query: 271 KNKLASGMLLTIDNQVDATTGTIKLKAQFPNTDYALFPNQFVNIRMLLDTRKDAVTIPTA 330 G + ++DN++D +GTI+++A F N + L P + +R+ AV I Sbjct: 259 DGYSRKGRVGSVDNRLDTASGTIRVRAIFDNANSQLVPGLYARVRLGGGEPHQAVLIDEK 318 Query: 331 ALQRGSKGLFVYVVKSDNTVTVRNVKTGPAQGDITAIEDGVASGETVVVDGIDRLREGAK 390 A+ FV V DN R V G + IE G+ GE +VV+G+ R+R G Sbjct: 319 AIGTDQDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQPGERIVVNGLQRIRPGDA 378 Query: 391 VEPVV 395 + P V Sbjct: 379 ITPTV 383 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 126 bits (316), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 99/339 (29%), Positives = 161/339 (47%), Gaps = 11/339 (3%) Query: 65 VGVAVARISDVNIYLTGLGGVTPTATATVRSRVDGQLMKLHFKEGQLVKEGDLLAELDAR 124 +GV V ++ G +P A VR +V+G + F+EG VK G +L +++ Sbjct: 41 LGVVTVHAQPVELHTELPGRTSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPA 100 Query: 125 PYQVAVTQAEGQLARDQAQLGAARLDLQRYRTLLSQDSIASQQVDTQAALVKQLEGTVKS 184 YQ A + LA+ +A + ARL RY+ L++ +++ Q D A + + E V S Sbjct: 101 SYQAAYDSSVAALAKAEASVRTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGS 160 Query: 185 DQGNLASARLQLTYSRVTAPVSGRLGLRQVDIGNIVHSSDTNGIVIITQVQPI------- 237 + N+ ++R+ L Y+R+ AP+SGR+G V G +V ++ T + I Q+ PI Sbjct: 161 ARANVQTSRINLDYARLDAPISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDVTQS 220 Query: 238 -TAVFSIPEDNIPAVMKQLQAGKKLPTDAWDRDQKNKLASGMLLTIDNQVDATTGTIKLK 296 T ++ E +++ G K+ + + G L D V+ TG I L+ Sbjct: 221 STTWLNLKEALANGQLEKSGNGAKV--RLVLENGRTYAQEGTLQFSDVTVNQDTGAITLR 278 Query: 297 AQFPNTDYALFPNQFVNIRMLLDTRKDAVTIPTAALQRGSKGLFV-YVVKSDNTVTVRNV 355 A FPN L P +V + R+ + +P A+ R G V +VV + V R + Sbjct: 279 AIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRAL 338 Query: 356 KTGPAQGDITAIEDGVASGETVVVDGIDRLREGAKVEPV 394 T A GD + G+ G+ +VVDG R GAKV+ V Sbjct: 339 VTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAGAKVKVV 377 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,662 Number of extensions: 50037 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 11 Length of query: 500 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 411 Effective length of database: 1,198,455 Effective search space: 492565005 Effective search space used: 492565005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)