BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0152.p (415 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 651 0.0 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 345 5e-96 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 342 2e-95 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 123 2e-29 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 123 3e-29 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 651 bits (1679), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/356 (91%), Positives = 338/356 (94%) Query: 1 MNPSRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMT 60 MNPSR FILRPVATSLLMLAILLVGIVAY+ LPLSALPEVDYPTIQVVTLYPGASPDVMT Sbjct: 1 MNPSRQFILRPVATSLLMLAILLVGIVAYRLLPLSALPEVDYPTIQVVTLYPGASPDVMT 60 Query: 61 SSVTAPLERQFGQMPGLNQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLP 120 SSVTAPLERQFGQMPGL QMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAG NLLP Sbjct: 61 SSVTAPLERQFGQMPGLKQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGSNLLP 120 Query: 121 SDLPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSGG 180 SDLP PP+YSKVNPADTPI+TLAVTS T+PLP+VQDLIDTR+AQKISQLSGVGLVSLSGG Sbjct: 121 SDLPMPPVYSKVNPADTPIITLAVTSNTMPLPKVQDLIDTRIAQKISQLSGVGLVSLSGG 180 Query: 181 QRPAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDGPARASTIDANDQLKSADE 240 QRPAVRMQLNPRA+AALG+NLDD+RTAIGNANVNQAKGSFDGP RASTIDANDQL+SADE Sbjct: 181 QRPAVRMQLNPRAVAALGLNLDDVRTAIGNANVNQAKGSFDGPTRASTIDANDQLRSADE 240 Query: 241 YRNLIIGYKNGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPGANVIAVVDSI 300 YRNLII YKNGAPIRVSDVADVVD AENVRLGAWAN AVIVNIQRQPGANVIAVVDSI Sbjct: 241 YRNLIIAYKNGAPIRVSDVADVVDGAENVRLGAWANTQPAVIVNIQRQPGANVIAVVDSI 300 Query: 301 KKILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMVIXELLLSI 356 KKILP LQASLPGAIDVHILTDRTTTIRASVDDVKFELLLS+ LVVMVI L ++ Sbjct: 301 KKILPQLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSVALVVMVIFVFLRNV 356 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 345 bits (884), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 180/368 (48%), Positives = 256/368 (69%), Gaps = 12/368 (3%) Query: 1 MNPSRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMT 60 M+ S FI RP+ T+LL +AILLVG+ + LP++ LP+VD+PTIQV PG SP+ M Sbjct: 1 MSVSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMA 60 Query: 61 SSVTAPLERQFGQMPGLNQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLP 120 S+V PLERQF + GL+QM+S S+ G++ ITLQF+L+ ++D A +VQAAINA LP Sbjct: 61 SNVAQPLERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLP 120 Query: 121 SDLPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSGG 180 ++LP PP + K+NPAD+PI+ ++V S LPL V D D LAQ+ISQ+SGVGLV+++G Sbjct: 121 ANLPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQ 180 Query: 181 QRPAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDGPARASTIDANDQLKSADE 240 Q+PAVR+Q++P +A LG++L+DIR I VNQ KG+ DG +A T+ NDQL A++ Sbjct: 181 QKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQ 240 Query: 241 YRNLIIGYKNGAPIRVSDVADVVDAAENVRLGAWA------------NDSAAVIVNIQRQ 288 + ++++ ++NGAPIRV D+ VD EN ++ AWA + ++++ I +Q Sbjct: 241 WNDMVLAWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGRSIVLAITKQ 300 Query: 289 PGANVIAVVDSIKKILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMV 348 PGANVI V IK +P L+A++P AI V+ L DRT TIRASV+DV+F L+L+I LVVMV Sbjct: 301 PGANVIETVGRIKAAMPRLRAAIPAAIHVNTLIDRTQTIRASVEDVEFTLVLTIALVVMV 360 Query: 349 IXELLLSI 356 I L ++ Sbjct: 361 IFVFLRNV 368 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 342 bits (878), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 175/353 (49%), Positives = 246/353 (69%) Query: 1 MNPSRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMT 60 MN S FI RP+AT+LL + I L GIVA++ LP+S LP+VDYPTI + PGASP+ M Sbjct: 1 MNISLPFIQRPIATTLLTIGIALAGIVAFRLLPVSPLPQVDYPTISISASMPGASPETMA 60 Query: 61 SSVTAPLERQFGQMPGLNQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLP 120 ++V PLER G + G+ +M+STSS ++ ITLQF+L +D A ++VQAA+NA NLLP Sbjct: 61 ATVATPLERAMGAIAGVTEMTSTSSQNSTRITLQFDLSRDIDGAARDVQAALNAARNLLP 120 Query: 121 SDLPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSGG 180 + +P+ P Y KVNPAD PIL LA+TS T+ ++ D D+ +AQK+SQ+ GVG VS+ G Sbjct: 121 TGMPSNPTYRKVNPADAPILILAMTSDTMTQGQMYDAADSIVAQKLSQVRGVGQVSVGGS 180 Query: 181 QRPAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDGPARASTIDANDQLKSADE 240 +PAVR++LNP A+ G+ D+RTA+ N N+ KG + R I ANDQ K+A E Sbjct: 181 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKGLLEDQDRNWQIYANDQAKTAAE 240 Query: 241 YRNLIIGYKNGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPGANVIAVVDSI 300 Y LI+ Y+NG P+RV DVA+V D+ +VR +N +V++ I RQPGAN+I VD++ Sbjct: 241 YAPLIVSYRNGTPVRVQDVAEVRDSVADVRNAGSSNGKPSVLIIINRQPGANIIDTVDNV 300 Query: 301 KKILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMVIXELL 353 +++LP LQAS+P AID+ ++ DRT TIRAS+ + +F L+LS+ LV+MV+ L Sbjct: 301 RELLPQLQASIPSAIDMKVVLDRTPTIRASLKETEFTLILSVALVIMVVFLFL 353 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 108/361 (29%), Positives = 185/361 (51%), Gaps = 14/361 (3%) Query: 4 SRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMTSSV 63 +R FI RP+ +L + ++L GIV+ LP++ P + P I + YPGAS + +V Sbjct: 2 ARFFIDRPIFAWVLAIIVMLAGIVSVLTLPIAQYPAIAPPAIAITANYPGASAKTLEDTV 61 Query: 64 TAPLERQFGQMPGLNQMSSTS-SGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLPSD 122 T +E++ + L+ M+STS S G+ ITL F + D A+ +VQ ++ LLPS+ Sbjct: 62 TQIIEQKMKGLDRLSYMASTSESSGSVTITLTFENGTNPDTAQVQVQNKLSLATPLLPSE 121 Query: 123 LPAPPIYSKVNPADTPILT-LAVTSR--TLPLPRVQDLIDTRLAQKISQLSGVGLVSLSG 179 + I V + T L LA TS ++ + D + + IS++ GVG +L G Sbjct: 122 VQQQGI--TVTKSATNFLNVLAFTSEDGSMSGSDLSDYVAANVQDAISRVEGVGDTTLFG 179 Query: 180 GQRPAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDG-PARA-----STIDAND 233 Q A+R+ L+P + + G+ D++ AI N + G G PA +TI A Sbjct: 180 SQY-AMRIWLDPNKLNSYGLTPLDVKNAIQAQNAQVSSGQLGGLPAAENQQINATITAQT 238 Query: 234 QLKSADEYRNLII-GYKNGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPGAN 292 +L++AD+++N+++ GA +++ DVA + +E+ N A + I+ GAN Sbjct: 239 RLRTADQFQNIVLRTLTGGAQVKLRDVARIELGSESYTSVGRYNGKPAAGLAIKLATGAN 298 Query: 293 VIAVVDSIKKILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMVIXEL 352 + V +I K + L+ P + V D T +R S+++V L+ ++VLV +V+ Sbjct: 299 ALDTVKAIDKRVAELEKFFPTGMKVQKPYDTTPFVRISIEEVVRTLIEAVVLVFLVMYLF 358 Query: 353 L 353 L Sbjct: 359 L 359 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 123 bits (308), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 108/368 (29%), Positives = 191/368 (51%), Gaps = 20/368 (5%) Query: 4 SRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMTSSV 63 +R FI RP+ ++ + I+L G+++ + L L P++ P + + Y GAS + SV Sbjct: 2 ARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDSV 61 Query: 64 TAPLERQFGQMPGLNQM-SSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLPSD 122 T +E+Q + L M +S+SS G++ ++L F + D+A+ +VQ + LP Sbjct: 62 TQVIEQQMKGLDNLQYMSASSSSAGSATVSLTFTAGTNPDVAQMQVQNKLQQVTARLPQA 121 Query: 123 LPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQ-----DLIDTRLAQKISQLSGVGLVSL 177 + + V A T IL V S T PR+ D I + L +IS++ GVG V+ Sbjct: 122 VQNNGV--TVTKASTDILM--VLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVT- 176 Query: 178 SGGQRPAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDG-PARA-----STIDA 231 + G A+R+ ++P + + DI +A+ N + G PA+ +TI A Sbjct: 177 AFGTNYAMRIWIDPAKLQKYALMPSDITSALQAQNTEVSAGEIGALPAQPGQQINATITA 236 Query: 232 NDQLKSADEYRNLIIGYK-NGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPG 290 +L + DE+RN+I+ +G+ +++SDVA V E+ ++ + N + + IQ G Sbjct: 237 RSKLTTPDEFRNIIVKSSVDGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATG 296 Query: 291 ANVIAVVDSIKKILPTLQASLPGAIDV--HILTDRTTTIRASVDDVKFELLLSIVLVVMV 348 AN +A D++KK L L+ P ++ + +I D T +R S+++V L+ +IVLVV++ Sbjct: 297 ANALATADAVKKKLAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLI 356 Query: 349 IXELLLSI 356 + L +I Sbjct: 357 MYLFLQNI 364 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,120,078 Number of extensions: 41455 Number of successful extensions: 186 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 11 Length of query: 415 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 327 Effective length of database: 1,203,190 Effective search space: 393443130 Effective search space used: 393443130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)